Secretion and processing of insulin precursors in yeast.
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Metabolic engineering of Saccharomyces cerevisiaeExpression of a human proprotein processing enzyme: correct cleavage of the von Willebrand factor precursor at a paired basic amino acid siteCell factories for insulin productionProinsulin processing by the subtilisin-related proprotein convertases furin, PC2, and PC3Production of recombinant human proinsulin in the milk of transgenic mice.Use of a synthetic peptide antigen to generate antisera reactive with a proteolytic processing site in native human proinsulin: demonstration of cleavage within clathrin-coated (pro)secretory vesicles.Expression of insulin in yeast: the importance of molecular adaptation for secretion and conversion.Processing of proopiomelanocortin by insulin secretory granule proinsulin processing endopeptidases.YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae.Efficient targeting to storage granules of human proinsulins with altered propeptide domain.Posttranslational processing of the prohormone-cleaving Kex2 protease in the Saccharomyces cerevisiae secretory pathwayIdentification of a cDNA encoding a second putative prohormone convertase related to PC2 in AtT20 cells and islets of LangerhansIsolation and functional expression of a mammalian prohormone processing enzyme, murine prohormone convertase 1.Plant-made pharmaceuticals for the prevention and treatment of autoimmune diseases: where are we?Biotechnology of non-Saccharomyces yeasts--the ascomycetes.Genetic basis of dyslipidemia in disease precipitation of coronary artery disease (CAD) associated type 2 diabetes mellitus (T2DM).Pursuit of a perfect insulin.Construction of a recombinant human insulin expression vector for mammary gland-specific expression in buffalo (Bubalus bubalis) mammary epithelial cell line.Saccharomyces cerevisiae Shuttle vectors.A note on the primary structure and expression of an Erwinia carotovora polygalacturonase-encoding gene (peh1) in Escherichia coli and Saccharomyces cerevisiae.Insulin-From its Discovery to the Industrial Synthesis of Modern Insulin Analogues.Expression and secretion of Bacillus amyloliquefaciens alpha-amylase by using the yeast pheromone alpha-factor promoter and leader sequence in Saccharomyces cerevisiae.The genetics of a putative social trait in natural populations of yeast.Dual-Color Monitoring Overcomes the Limitations of Single Bioluminescent Reporters in Fast-Growing Microbes and Reveals Phase-Dependent Protein Productivity during the Metabolic Rhythms of Saccharomyces cerevisiae.Application of simple fed-batch technique to high-level secretory production of insulin precursor using Pichia pastoris with subsequent purification and conversion to human insulinHuman insulin production from a novel mini-proinsulin which has high receptor-binding activity.Restriction site free cloning (RSFC) plasmid family for seamless, sequence independent cloning in Pichia pastorisSequence requirements for processing of proinsulin in transfected mouse pituitary AtT20 cells.A member of the eukaryotic subtilisin family (PC3) has the enzymic properties of the type 1 proinsulin-converting endopeptidase.Differential rates of conversion of rat proinsulins I and II. Evidence for slow cleavage at the B-chain/C-peptide junction of proinsulin IIPlaying catch-up with Escherichia coli: using yeast to increase success rates in recombinant protein production experiments.Display of human proinsulin on the Bacillus subtilis spore surface for oral administration.The inhibition of proinsulin-processing endopeptidase activities by active-site-directed peptidesEndoproteolytic processing of recombinant proalbumin variants by the yeast Kex2 protease.A single-chain insulin-like growth factor I/insulin hybrid binds with high affinity to the insulin receptorDesign and folding of [GluA4(ObetaThrB30)]insulin ("ester insulin"): a minimal proinsulin surrogate that can be chemically converted into human insulinMulti-capillary column-ion mobility spectrometry of volatile metabolites emitted by Saccharomyces cerevisiae.Biomimetic synthesis of lispro insulin via a chemically synthesized "mini-proinsulin" prepared by oxime-forming ligation.Recombinant expression of an insulin-like peptide 3 (INSL3) precursor and its enzymatic conversion to mature human INSL3.Disruption of the KEX1 gene in Pichia pastoris allows expression of full-length murine and human endostatin.
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Secretion and processing of insulin precursors in yeast.
description
article científic
@ca
article scientifique
@fr
articolo scientifico
@it
artigo científico
@pt
bilimsel makale
@tr
scientific article published on September 1986
@en
vedecký článok
@sk
vetenskaplig artikel
@sv
videnskabelig artikel
@da
vědecký článek
@cs
name
Secretion and processing of insulin precursors in yeast.
@en
Secretion and processing of insulin precursors in yeast.
@nl
type
label
Secretion and processing of insulin precursors in yeast.
@en
Secretion and processing of insulin precursors in yeast.
@nl
prefLabel
Secretion and processing of insulin precursors in yeast.
@en
Secretion and processing of insulin precursors in yeast.
@nl
P2093
P2860
P356
P1476
Secretion and processing of insulin precursors in yeast.
@en
P2093
Forstrom J
P2860
P304
P356
10.1073/PNAS.83.18.6766
P407
P577
1986-09-01T00:00:00Z