Reliability of real-time reverse-transcription PCR in clinical diagnostics: gold standard or substandard?
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Detection of miRNAs with a nanopore single-molecule counterValidation of kinetics similarity in qPCRA portable, shock-proof, surface-heated droplet PCR system for Escherichia coli detection.RNA pre-amplification enables large-scale RT-qPCR gene-expression studies on limiting sample amountsExternal oligonucleotide standards enable cross laboratory comparison and exchange of real-time quantitative PCR data.Assessing the performance capabilities of LRE-based assays for absolute quantitative real-time PCR.Shape based kinetic outlier detection in real-time PCR.A Java program for LRE-based real-time qPCR that enables large-scale absolute quantification.Differential expression of CHL1 gene during development of major human cancers.Importance of pre-analytical steps for transcriptome and RT-qPCR analyses in the context of the phase II randomised multicentre trial REMAGUS02 of neoadjuvant chemotherapy in breast cancer patientsGrowth and virulence properties of biofilm-forming Salmonella enterica serovar typhimurium under different acidic conditions.Kinin B1 receptor antagonism is equally efficient as angiotensin receptor 1 antagonism in reducing renal fibrosis in experimental obstructive nephropathy, but is not additive.Evaluation and validation of a robust single cell RNA-amplification protocol through transcriptional profiling of enriched lung cancer initiating cells.Custom design of a GeXP multiplexed assay used to assess expression profiles of inflammatory gene targets in normal colon, polyp, and tumor tissue.Multiplexed quantification of nucleic acids with large dynamic range using multivolume digital RT-PCR on a rotational SlipChip tested with HIV and hepatitis C viral loadTheoretical design and analysis of multivolume digital assays with wide dynamic range validated experimentally with microfluidic digital PCRDigital Droplet PCR for the Absolute Quantification of Exon Skipping Induced by Antisense Oligonucleotides in (Pre-)Clinical Development for Duchenne Muscular Dystrophy.Mechanistic evaluation of the pros and cons of digital RT-LAMP for HIV-1 viral load quantification on a microfluidic device and improved efficiency via a two-step digital protocolG-quadruplex-generating polymerase chain reaction for visual colorimetric detection of amplicons.Non-coding RNAs: identification of cancer-associated microRNAs by gene profiling.Quantitative polymerase chain reaction (PCR) for detection of aquatic animal pathogens in a diagnostic laboratory setting.Extended lymphadenectomy in colon cancer is debatable.Application of unique sequence index (USI) barcode to gene expression profiling in gastric adenocarcinoma.Identification of reference genes for qRT-PCR in granulosa cells of healthy women and polycystic ovarian syndrome patients.Transcriptome Profiling in Human Diseases: New Advances and PerspectivesMeasurable impact of RNA quality on gene expression results from quantitative PCR.How good is a PCR efficiency estimate: Recommendations for precise and robust qPCR efficiency assessments.Impact of long-term storage on stability of standard DNA for nucleic acid-based methods.Reproducibility of biomedical research - The importance of editorial vigilanceMolecular diagnostics: the changing culture of medical microbiology.A novel poisson distribution-based approach for testing boundaries of real-time PCR assays for food pathogen quantification.Mechanisms of degradation of DNA standards for calibration function during storage.Detection of small RNA molecules by a combination of branched rolling circle amplification and bioluminescent pyrophosphate assay.Assessment of suitable reference genes for RT-qPCR studies in chronic rhinosinusitis.Colorimetric molecular diagnosis of the HIV gag gene using DNAzyme and a complementary DNA-extended primer.
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Reliability of real-time reverse-transcription PCR in clinical diagnostics: gold standard or substandard?
description
article científic
@ca
article scientifique
@fr
articolo scientifico
@it
artigo científico
@pt
bilimsel makale
@tr
scientific article published on March 2009
@en
vedecký článok
@sk
vetenskaplig artikel
@sv
videnskabelig artikel
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vědecký článek
@cs
name
Reliability of real-time rever ...... gold standard or substandard?
@en
Reliability of real-time rever ...... gold standard or substandard?
@nl
type
label
Reliability of real-time rever ...... gold standard or substandard?
@en
Reliability of real-time rever ...... gold standard or substandard?
@nl
prefLabel
Reliability of real-time rever ...... gold standard or substandard?
@en
Reliability of real-time rever ...... gold standard or substandard?
@nl
P356
P1476
Reliability of real-time rever ...... gold standard or substandard?
@en
P2093
Jamie Murphy
P304
P356
10.1586/14737159.9.2.187
P577
2009-03-01T00:00:00Z