DNA binding specificity of the EcoRV restriction endonuclease is increased by Mg2+ binding to a metal ion binding site distinct from the catalytic center of the enzyme.
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Structure and function of type II restriction endonucleasesUsing electrostatic potentials to predict DNA-binding sites on DNA-binding proteins.Divalent cations and the electrostatic potential around DNA: Monte Carlo and Poisson-Boltzmann calculations.Type II restriction endonucleases--a historical perspective and moreEnzymatic cleavage of type II restriction endonucleases on the 2'-O-methyl nucleotide and phosphorothioate substituted DNA.Expression and purification of a single-chain Type IV restriction enzyme Eco94GmrSD and determination of its substrate preference.Introduction of asymmetry in the naturally symmetric restriction endonuclease EcoRV to investigate intersubunit communication in the homodimeric protein.A conserved nuclease domain in the archaeal Holliday junction resolving enzyme Hjc.Crosslinking the EcoRV restriction endonuclease across the DNA-binding site reveals transient intermediates and conformational changes of the enzyme during DNA binding and catalytic turnoverProbing of the spliceosome with site-specifically derivatized 5' splice site RNA oligonucleotidesSequence specific interaction of Mycobacterium smegmatis topoisomerase I with duplex DNA.Site-directed mutagenesis of conserved aspartates, glutamates and arginines in the active site region of Escherichia coli DNA topoisomerase I.Influence of the phosphate backbone on the recognition and hydrolysis of DNA by the EcoRV restriction endonuclease. A study using oligodeoxynucleotide phosphorothioates.Probing the indirect readout of the restriction enzyme EcoRV. Mutational analysis of contacts to the DNA backbone.Asp-59 is not important for the catalytic activity of the restriction endonuclease EcoRI.The effect of Me2+ cofactors at the initial stages of V(D)J recombination.Multiple roles for divalent metal ions in DNA transposition: distinct stages of Tn10 transposition have different Mg2+ requirements.Factors responsible for target site selection in Tn10 transposition: a role for the DDE motif in target DNA capture.From Bioengineering to CRISPR/Cas9 - A Personal Retrospective of 20 Years of Research in Programmable Genome Targeting.Kinetic and stoichiometric analysis for the binding of Escherichia coli ribonuclease HI to RNA-DNA hybrids using surface plasmon resonance.The Mechanism of DNA Cleavage by the Type II. Restriction Enzyme EcoRV: Asp36 Is Not Directly Involved in DNA Cleavage but Serves to Couple Indirect. Readout to Catalysis
P2860
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P2860
DNA binding specificity of the EcoRV restriction endonuclease is increased by Mg2+ binding to a metal ion binding site distinct from the catalytic center of the enzyme.
description
1995 nî lūn-bûn
@nan
1995年の論文
@ja
1995年論文
@yue
1995年論文
@zh-hant
1995年論文
@zh-hk
1995年論文
@zh-mo
1995年論文
@zh-tw
1995年论文
@wuu
1995年论文
@zh
1995年论文
@zh-cn
name
DNA binding specificity of the ...... atalytic center of the enzyme.
@en
type
label
DNA binding specificity of the ...... atalytic center of the enzyme.
@en
prefLabel
DNA binding specificity of the ...... atalytic center of the enzyme.
@en
P2093
P356
P1433
P1476
DNA binding specificity of the ...... catalytic center of the enzyme
@en
P2093
P304
P356
10.1021/BI00018A028
P407
P577
1995-05-01T00:00:00Z