Purification, biochemical characterization and protein-DNA interactions of the I-CreI endonuclease produced in Escherichia coli.
about
Mutations altering the cleavage specificity of a homing endonucleaseThe C-terminal loop of the homing endonuclease I-CreI is essential for site recognition, DNA binding and cleavageCrystal structures of I-SceI complexed to nicked DNA substrates: snapshots of intermediates along the DNA cleavage reaction pathwayMolecular basis of engineered meganuclease targeting of the endogenous human RAG1 locus5'-Cytosine-Phosphoguanine (CpG) Methylation Impacts the Activity of Natural and Engineered MeganucleasesNon-specific protein-DNA interactions control I-CreI target binding and cleavageMycobacterium tuberculosis RecA intein, a LAGLIDADG homing endonuclease, displays Mn(2+) and DNA-dependent ATPase activityThe RecA intein of Mycobacterium tuberculosis promotes cleavage of ectopic DNA sites. Implications for the dispersal of inteins in natural populationsComputer design of obligate heterodimer meganucleases allows efficient cutting of custom DNA sequences.A novel engineered meganuclease induces homologous recombination in yeast and mammalian cellsCrystal Structure of the Homing Endonuclease I-CvuI Provides a New Template for Genome Modification.Crystallization and preliminary X-ray diffraction analysis on the homing endonuclease I-Dmo-I in complex with its target DNA.Characterization of Mycobacterium leprae RecA intein, a LAGLIDADG homing endonuclease, reveals a unique mode of DNA binding, helical distortion, and cleavage compared with a canonical LAGLIDADG homing endonucleaseTargeted mutagenesis of a conserved anther-expressed P450 gene confers male sterility in monocots.Generation and analysis of mesophilic variants of the thermostable archaeal I-DmoI homing endonuclease.Rapid evolution of the DNA-binding site in LAGLIDADG homing endonucleases.Double-strand breaks repair by gene conversion in silkworm holocentric chromosomes.Purification and characterization of the DNA cleavage and recognition site of I-ScaI mitochondrial group I intron encoded endonuclease produced in Escherichia coli.Biochemical characterization of I-CmoeI reveals that this H-N-H homing endonuclease shares functional similarities with H-N-H colicinsConstruction of a recombinant adenovirus for efficient delivery of the I-SceI yeast endonuclease to human cells and its application in the in vivo cleavage of chromosomes to expose new potential telomeresBiochemical and mutagenic analysis of I-CreII reveals distinct but important roles for both the H-N-H and GIY-YIG motifsFunctional characterization of isoschizomeric His-Cys box homing endonucleases from Naegleria.Mutational analysis of active-site residues in the Mycobacterium leprae RecA intein, a LAGLIDADG homing endonuclease: Asp(122) and Asp(193) are crucial to the double-stranded DNA cleavage activity whereas Asp(218) is not.In vitro analysis of the relationship between endonuclease and maturase activities in the bi-functional group I intron-encoded protein, I-AniI.F-CphI represents a new homing endonuclease family using the Endo VII catalytic motif
P2860
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P2860
Purification, biochemical characterization and protein-DNA interactions of the I-CreI endonuclease produced in Escherichia coli.
description
1997 nî lūn-bûn
@nan
1997年の論文
@ja
1997年学术文章
@wuu
1997年学术文章
@zh-cn
1997年学术文章
@zh-hans
1997年学术文章
@zh-my
1997年学术文章
@zh-sg
1997年學術文章
@yue
1997年學術文章
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1997年學術文章
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name
Purification, biochemical char ...... produced in Escherichia coli.
@en
type
label
Purification, biochemical char ...... produced in Escherichia coli.
@en
prefLabel
Purification, biochemical char ...... produced in Escherichia coli.
@en
P2093
P2860
P356
P1476
Purification, biochemical char ...... produced in Escherichia coli.
@en
P2093
P2860
P304
P356
10.1093/NAR/25.19.3767
P407
P577
1997-10-01T00:00:00Z