A simple in vitro Tn7-based transposition system with low target site selectivity for genome and gene analysis
about
Major structural differences and novel potential virulence mechanisms from the genomes of multiple campylobacter speciesConditional knockout mice reveal distinct functions for the global transcriptional coactivators CBP and p300 in T-cell developmentSimple and straightforward construction of a mouse gene targeting vector using in vitro transposition reactionsKu interacts with telomerase RNA to promote telomere addition at native and broken chromosome ends.Genome-wide transposon mutagenesis in Saccharomyces cerevisiae and Candida albicansPromoter activation by CII, a potent transcriptional activator from bacteriophage 186.Tn7-based genome-wide random insertional mutagenesis of Candida glabrata.Comprehensive mutational analysis of a herpesvirus gene in the viral genome context reveals a region essential for virus replicationLarge-scale mutagenesis of the yeast genome using a Tn7-derived multipurpose transposon.Global transposon mutagenesis and essential gene analysis of Helicobacter pylori.Functional mapping of Cre recombinase by pentapeptide insertional mutagenesis.Insertion of green fluorescent protein into nonstructural protein 5A allows direct visualization of functional hepatitis C virus replication complexes.In vivo Himar1-based transposon mutagenesis of Francisella tularensis.Genomic analysis of insertion behavior and target specificity of mini-Tn7 and Tn3 transposons in Saccharomyces cerevisiaeRevealing domain structure through linker-scanning analysis of the murine leukemia virus (MuLV) RNase H and MuLV and human immunodeficiency virus type 1 integrase proteinsRandom screening for dominant-negative mutants of the cytomegalovirus nuclear egress protein M50Refined annotation and assembly of the Tetrahymena thermophila genome sequence through EST analysis, comparative genomic hybridization, and targeted gap closureTransposon Tn7 preferentially inserts into GAA*TTC triplet repeats under conditions conducive to Y*R*Y triplex formation.Characterization of the CI repressor protein encoded by the temperate lactococcal phage TP901-1.Systematic sequencing of cDNA clones using the transposon Tn5Domain structure of HrpE, the Hrp pilus subunit of Xanthomonas campestris pv. vesicatoria.M94 is essential for the secondary envelopment of murine cytomegalovirus.A large-scale complex haploinsufficiency-based genetic interaction screen in Candida albicans: analysis of the RAM network during morphogenesis.Formation of a nucleoprotein complex containing Tn7 and its target DNA regulates transposition initiation.Efficient insertion mutagenesis strategy for bacterial genomes involving electroporation of in vitro-assembled DNA transposition complexes of bacteriophage muFunctional analysis of the triplex proteins (VP19C and VP23) of herpes simplex virus type 1A direct transposon insertion tool for modification and functional analysis of viral genomes.Protein kinase A regulates constitutive expression of small heat-shock genes in an Msn2/4p-independent and Hsf1p-dependent manner in Saccharomyces cerevisiae.Mass spectrometry-based proteomic approaches for discovery of HIV-host interactions.Inter- and intra-specific pan-genomes of Borrelia burgdorferi sensu lato: genome stability and adaptive radiation.Role of capsule and O antigen in the virulence of uropathogenic Escherichia coli.Genome-wide synthetic genetic screening by transposon mutagenesis in Candida albicansAvoiding the ends: internal epitope tagging of proteins using transposon Tn7Transposon Tn7 directs transposition into the genome of filamentous bacteriophage M13 using the element-encoded TnsE protein.Rapid site-directed domain scanning mutagenesis of enteropathogenic Escherichia coli espDWhat limits the efficiency of double-strand break-dependent stress-induced mutation in Escherichia coli?The helix-turn-helix motif of the coliphage 186 immunity repressor binds to two distinct recognition sequences.In vitro mutagenesis of Bacillus subtilis by using a modified Tn7 transposon with an outward-facing inducible promoter.Haploinsufficiency-based large-scale forward genetic analysis of filamentous growth in the diploid human fungal pathogen C.albicansThe bacterial transposon Tn7 causes premature polyadenylation of mRNA in eukaryotic organisms: TAGKO mutagenesis in filamentous fungi.
P2860
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P2860
A simple in vitro Tn7-based transposition system with low target site selectivity for genome and gene analysis
description
2000 nî lūn-bûn
@nan
2000年の論文
@ja
2000年論文
@yue
2000年論文
@zh-hant
2000年論文
@zh-hk
2000年論文
@zh-mo
2000年論文
@zh-tw
2000年论文
@wuu
2000年论文
@zh
2000年论文
@zh-cn
name
A simple in vitro Tn7-based tr ...... y for genome and gene analysis
@en
A simple in vitro Tn7-based tr ...... for genome and gene analysis.
@nl
type
label
A simple in vitro Tn7-based tr ...... y for genome and gene analysis
@en
A simple in vitro Tn7-based tr ...... for genome and gene analysis.
@nl
prefLabel
A simple in vitro Tn7-based tr ...... y for genome and gene analysis
@en
A simple in vitro Tn7-based tr ...... for genome and gene analysis.
@nl
P2093
P2860
P356
P1476
A simple in vitro Tn7-based tr ...... y for genome and gene analysis
@en
P2093
A E Stellwagen
E A Raleigh
F J Stewart
P2860
P304
P356
10.1093/NAR/28.5.1067
P407
P577
2000-03-01T00:00:00Z