An efficient and targeted gene integration system for high-level antibody expression.
about
Optimized signal peptides for the development of high expressing CHO cell linesCombination of FACS and homologous recombination for the generation of stable and high-expression engineered cell linesA rapid Flp-In system for expression of secreted H5N1 influenza hemagglutinin vaccine immunogen in mammalian cellsRecombinant protein expression by targeting pre-selected chromosomal loci.Comprehensive engineering of Escherichia coli for enhanced expression of IgG antibodiesRapid establishment of a HEK 293 cell line expressing FVIII-BDD using AAV site-specific integration plasmids.A novel regulatory element (E77) isolated from CHO-K1 genomic DNA enhances stable gene expression in Chinese hamster ovary cellsFISH-Based Analysis of Clonally Derived CHO Cell Populations Reveals High Probability for Transgene Integration in a Terminal Region of Chromosome 1 (1q13).Advances in Mammalian cell line development technologies for recombinant protein production.CHO cells in biotechnology for production of recombinant proteins: current state and further potential.An 'omics approach towards CHO cell engineering.CRISPR/Cas9-mediated genome engineering of CHO cell factories: Application and perspectives.Utilization of Site-Specific Recombination in Biopharmaceutical Production.Effects of lysosomal biotherapeutic recombinant protein expression on cell stress and protease and general host cell protein release in Chinese hamster ovary cells.Accurate comparison of antibody expression levels by reproducible transgene targeting in engineered recombination-competent CHO cells.Recent advances in recombinant protein production: BAC-based expression vectors, the bigger the better.Flipase-mediated cassette exchange in Sf9 insect cells for stable gene expression.Polyarginine peptide IND-1 enhances recombinant human bone morphogenetic protein-2 yield in mammalian cells.Bacterial artificial chromosomes improve recombinant protein production in mammalian cells.Site-specific integration in CHO cells mediated by CRISPR/Cas9 and homology-directed DNA repair pathway.Repeated integration of antibody genes into a pre-selected chromosomal locus of CHO cells using an accumulative site-specific gene integration system.Mutation of a cleavage site adjacent to the mature domain leads to increase in secreted mature BMP-2 with reduced activity.Evaluating the efficiency of phiC31 integrase-mediated monoclonal antibody expression in CHO cells.Strategies and Considerations for Improving Expression of "Difficult to Express" Proteins in CHO Cells.Coupling recombinase-mediated cassette exchange with somatic hypermutation for antibody affinity maturation in CHO cells.
P2860
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P2860
An efficient and targeted gene integration system for high-level antibody expression.
description
2007 nî lūn-bûn
@nan
2007年の論文
@ja
2007年学术文章
@wuu
2007年学术文章
@zh-cn
2007年学术文章
@zh-hans
2007年学术文章
@zh-my
2007年学术文章
@zh-sg
2007年學術文章
@yue
2007年學術文章
@zh
2007年學術文章
@zh-hant
name
An efficient and targeted gene integration system for high-level antibody expression.
@en
type
label
An efficient and targeted gene integration system for high-level antibody expression.
@en
prefLabel
An efficient and targeted gene integration system for high-level antibody expression.
@en
P2093
P1476
An efficient and targeted gene integration system for high-level antibody expression.
@en
P2093
P356
10.1016/J.JIM.2007.01.022
P577
2007-02-26T00:00:00Z