Site-specific integration in CHO cells mediated by CRISPR/Cas9 and homology-directed DNA repair pathway.
about
Genome Engineering with TALE and CRISPR Systems in NeuroscienceTransient overexpression of DNA adenine methylase enables efficient and mobile genome engineering with reduced off-target effects.A Knowledge-Based System for Display and Prediction of O-Glycosylation Network Behaviour in Response to Enzyme KnockoutsCRMAGE: CRISPR Optimized MAGE Recombineering.CRISPR/Cas9-mediated genome engineering of CHO cell factories: Application and perspectives.Utilization of Site-Specific Recombination in Biopharmaceutical Production.New Mammalian Expression Systems.A novel Bxb1 integrase RMCE system for high fidelity site-specific integration of mAb expression cassette in CHO Cells.Identifying and retargeting transcriptional hot spots in the human genome.High levels of histone H3 acetylation at the CMV promoter are predictive of stable expression in Chinese hamster ovary cells.Gene editing for cell engineering: trends and applications.Using the CRISPR/Cas9 system to understand neuropeptide biology and regulation.Homologous Recombination-Independent Large Gene Cassette Knock-in in CHO Cells Using TALEN and MMEJ-Directed Donor Plasmids.Development of a silicon limitation inducible expression system for recombinant protein production in the centric diatoms Thalassiosira pseudonana and Cyclotella cryptica.CRISPR/Cas9-induced transgene insertion and telomere-associated truncation of a single human chromosome for chromosome engineering in CHO and A9 cells.Yeast knockout library allows for efficient testing of genomic mutations for cell-free protein synthesis.Baicalein Reduces Oxidative Stress in CHO Cell Cultures and Improves Recombinant Antibody Productivity.Enhanced Genome Editing Tools For Multi-Gene Deletion Knock-Out Approaches Using Paired CRISPR sgRNAs in CHO Cells.A large-scale expression strategy for multimeric extracellular protein complexes using Drosophila S2 cells and its application to the recombinant expression of heterodimeric ligand-binding domains of taste receptor.Novel high-throughput cell-based hybridoma screening methodology using the Celigo Image Cytometer.Accelerated homology-directed targeted integration of transgenes in Chinese hamster ovary cells via CRISPR/Cas9 and fluorescent enrichment.Strategies and Considerations for Improving Expression of "Difficult to Express" Proteins in CHO Cells.Knockout of a difficult-to-remove CHO host cell protein, lipoprotein lipase, for improved polysorbate stability in monoclonal antibody formulations.Exploiting the CRISPR/Cas9 system to study alternative splicing in vivo: application to titin.Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems.Novel RNA-Binding Proteins Isolation by the RaPID Methodology.Development of a bacterial-based negative selection system for rapid screening of active single guide RNAs.A protocol for custom CRISPR Cas9 donor vector construction to truncate genes in mammalian cells using pcDNA3 backbone.A reference genome of the Chinese hamster based on a hybrid assembly strategyChimeric Antigen Receptors Based on Low Affinity Mutants of FcεRI Re-direct T Cell Specificity to Cells Expressing Membrane IgE
P2860
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P2860
Site-specific integration in CHO cells mediated by CRISPR/Cas9 and homology-directed DNA repair pathway.
description
2015 nî lūn-bûn
@nan
2015年の論文
@ja
2015年論文
@yue
2015年論文
@zh-hant
2015年論文
@zh-hk
2015年論文
@zh-mo
2015年論文
@zh-tw
2015年论文
@wuu
2015年论文
@zh
2015年论文
@zh-cn
name
Site-specific integration in C ...... y-directed DNA repair pathway.
@en
Site-specific integration in C ...... y-directed DNA repair pathway.
@nl
type
label
Site-specific integration in C ...... y-directed DNA repair pathway.
@en
Site-specific integration in C ...... y-directed DNA repair pathway.
@nl
prefLabel
Site-specific integration in C ...... y-directed DNA repair pathway.
@en
Site-specific integration in C ...... y-directed DNA repair pathway.
@nl
P2860
P50
P356
P1433
P1476
Site-specific integration in C ...... y-directed DNA repair pathway.
@en
P2093
Jae Seong Lee
P2860
P2888
P356
10.1038/SREP08572
P407
P577
2015-02-25T00:00:00Z
P5875
P6179
1041544591