about
An efficient one-step site-directed deletion, insertion, single and multiple-site plasmid mutagenesis protocol.The splicing regulator Sam68 binds to a novel exonic splicing silencer and functions in SMN2 alternative splicing in spinal muscular atrophyA New Autocatalytic Activation Mechanism for Cysteine Proteases Revealed by Prevotella intermedia Interpain ASolution Structure and Backbone Dynamics of Streptopain: INSIGHT INTO DIVERSE SUBSTRATE SPECIFICITYCrystal Structure of the Heme d 1 Biosynthesis Enzyme NirE in Complex with Its Substrate Reveals New Insights into the Catalytic Mechanism of S -Adenosyl-l-methionine-dependent Uroporphyrinogen III MethyltransferasesThe function of the yeast molecular chaperone Sse1 is mechanistically distinct from the closely related hsp70 family.Mlc1p promotes septum closure during cytokinesis via the IQ motifs of the vesicle motor Myo2p.Poliovirus RNA-dependent RNA polymerase (3Dpol): structural, biochemical, and biological analysis of conserved structural motifs A and BThe congenital cataract-linked A2V mutation impairs tetramer formation and promotes aggregation of βB2-crystallinMycobacterium tuberculosis RecA intein, a LAGLIDADG homing endonuclease, displays Mn(2+) and DNA-dependent ATPase activityThe RecA intein of Mycobacterium tuberculosis promotes cleavage of ectopic DNA sites. Implications for the dispersal of inteins in natural populationsAn efficient one-step site-directed and site-saturation mutagenesis protocolFine mapping and characterization of the Rous sarcoma virus Pr76gag late assembly domainModulation of the enzymatic efficiency of ferredoxin-NADP(H) reductase by the amino acid volume around the catalytic site.A novel heme-responsive element mediates transcriptional regulation in Caenorhabditis elegans.hgpB, a gene encoding a second Haemophilus influenzae hemoglobin- and hemoglobin-haptoglobin-binding protein.Proper processing of avian sarcoma/leukosis virus capsid proteins is required for infectivityLyn kinase and ZAP70 are substrates of PTPROt in B-cells: Lyn inactivation by PTPROt sensitizes leukemia cells to VEGF-R inhibitor pazopanibAnalysis of the BarA/UvrY two-component system in Shewanella oneidensis MR-1Expression, purification and crystallization of CTB-MPR, a candidate mucosal vaccine component against HIV-1.Delivery of CdiA nuclease toxins into target cells during contact-dependent growth inhibitionPhoP-induced genes within Salmonella pathogenicity island 1.Genetic analysis of the herpes simplex virus type 1 UL20 protein domains involved in cytoplasmic virion envelopment and virus-induced cell fusion.Selection of orphan Rhs toxin expression in evolved Salmonella enterica serovar Typhimurium.A single serine residue determines selectivity to monovalent metal ions in metalloregulators of the MerR familyGenetic analysis of the CDI pathway from Burkholderia pseudomallei 1026bSequential activation of hypoxia-inducible factor 1 and specificity protein 1 is required for hypoxia-induced transcriptional stimulation of Abcc8.Identification and characterization of OGG1 mutations in patients with Alzheimer's disease.Altered Rous sarcoma virus Gag polyprotein processing and its effects on particle formation.Multiple biological roles associated with the Rous sarcoma virus 5' untranslated RNA U5-IR stem and loop.The toxin/immunity network of Burkholderia pseudomallei contact-dependent growth inhibition (CDI) systemsOctanoyl-homoserine lactone is the cognate signal for Burkholderia mallei BmaR1-BmaI1 quorum sensing.RBM45 homo-oligomerization mediates association with ALS-linked proteins and stress granulesDiversification of β-Augmentation Interactions between CDI Toxin/Immunity ProteinsThe herpes simplex virus UL20 protein functions in glycoprotein K (gK) intracellular transport and virus-induced cell fusion are independent of UL20 functions in cytoplasmic virion envelopmentAutoregulation of pituitary corticotroph SOCS-3 expression: characterization of the murine SOCS-3 promoter.CdiA promotes receptor-independent intercellular adhesion.Streptococcal pyrogenic exotoxin B-induced apoptosis in A549 cells is mediated through alpha(v)beta(3) integrin and Fas.Functional conservation of beta-hairpin DNA binding domains in the Mcm protein of Methanobacterium thermoautotrophicum and the Mcm5 protein of Saccharomyces cerevisiae.The C-terminal domain of A1/Bfl-1 regulates its anti-inflammatory function in human endothelial cells
P2860
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P2860
description
1996 nî lūn-bûn
@nan
1996年の論文
@ja
1996年論文
@yue
1996年論文
@zh-hant
1996年論文
@zh-hk
1996年論文
@zh-mo
1996年論文
@zh-tw
1996年论文
@wuu
1996年论文
@zh
1996年论文
@zh-cn
name
Site-directed mutagenesis using overlap extension PCR.
@en
type
label
Site-directed mutagenesis using overlap extension PCR.
@en
prefLabel
Site-directed mutagenesis using overlap extension PCR.
@en
P2093
P1476
Site-directed mutagenesis using overlap extension PCR.
@en
P2093
P304
P356
10.1385/0-89603-332-5:177
P407
P577
1996-01-01T00:00:00Z