Investigation of the active site of papain with fluorescent probes.
about
A kinetic and fluorimetric investigation of papain modified at tryptophan-69 and -177 by N-bromosuccinimideA reporter group delivery system with both absolute and selective specificity for thiol groups and an improved fluorescent probe containing the 7-nitrobenzo-2-oxa-1,3-diazole moiety4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole as a reactivity probe for the investigation of the thiol proteinases. evidence that ficin and bromelain may lack carboxyl groups conformationally equivalent to that of aspartic acid-158 of papain.The highly electrophilic character of 4-chloro-7-nitrobenzofurazan and possible consequences for its application as a protein-labelling reagent.Benzofurazans and benzofuroxans: biochemical and pharmacological properties.Impairment of the catalytic activity of Escherichia coli ribonucleic acid polymerase by a unique reaction of 4-chloro-7-nitrobenzofurazan.Anomalous reaction of 4-chloro-7-nitrobenzofurazan with thiol compounds.Evidence for a two-state transition in papain that may have no close analogue in ficin. Differences in the disposition of cationic sites and hydrophobic binding areas in the active centres of papain and ficin.Differences between the electric fields of the catalytic sites of papain and actinidin detected by using the thiol-located nitrobenzofurazan label as a spectroscopic reporter group.Ionization characteristics and chemical influences of aspartic acid residue 158 of papain and caricain determined by structure-related kinetic and computational techniques: multiple electrostatic modulators of active-centre chemistry.
P2860
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P2860
Investigation of the active site of papain with fluorescent probes.
description
1973 nî lūn-bûn
@nan
1973年の論文
@ja
1973年論文
@yue
1973年論文
@zh-hant
1973年論文
@zh-hk
1973年論文
@zh-mo
1973年論文
@zh-tw
1973年论文
@wuu
1973年论文
@zh
1973年论文
@zh-cn
name
Investigation of the active site of papain with fluorescent probes.
@en
Investigation of the active site of papain with fluorescent probes.
@nl
type
label
Investigation of the active site of papain with fluorescent probes.
@en
Investigation of the active site of papain with fluorescent probes.
@nl
prefLabel
Investigation of the active site of papain with fluorescent probes.
@en
Investigation of the active site of papain with fluorescent probes.
@nl
P2860
P356
P1433
P1476
Investigation of the active site of papain with fluorescent probes.
@en
P2860
P304
P356
10.1042/BJ1330679
P407
P577
1973-08-01T00:00:00Z