Exchange of single amino acids at different positions of a recombinant protein affects metabolic burden in Escherichia coli.

Exchange of single amino acids at different positions of a recombinant protein affects metabolic burden in Escherichia coli.

Item
http://www.wikidata.org/entity/Q43120768
Cellulolytic RoboLector - towards an automated high-throughput screening platform for recombinant cellulase expressionRespiration activity monitoring system for any individual well of a 48-well microtiter plate.Light-induced gene expression with photocaged IPTG for induction profiling in a high-throughput screening system.Easy to use and reliable technique for online dissolved oxygen tension measurement in shake flasks using infrared fluorescent oxygen-sensitive nanoparticles.Probing unnatural amino acid integration into enhanced green fluorescent protein by genetic code expansion with a high-throughput screening platform.Quasi-continuous parallel online scattered light, fluorescence and dissolved oxygen tension measurement combined with monitoring of the oxygen transfer rate in each well of a shaken microtiter plate.Maximizing the stability of metabolic engineering-derived whole-cell biocatalysts.Prediction of Escherichia coli expression performance in microtiter plates by analyzing only the temporal development of scattered light during culture.A particular silent codon exchange in a recombinant gene greatly influences host cell metabolic activity.Parallel use of shake flask and microtiter plate online measuring devices (RAMOS and BioLector) reduces the number of experiments in laboratory-scale stirred tank bioreactors.Online monitoring of dissolved oxygen tension in microtiter plates based on infrared fluorescent oxygen-sensitive nanoparticlesOptimizing recombinant protein expression via automated induction profiling in microtiter plates at different temperatures.Online measurement of the respiratory activity in shake flasks enables the identification of cultivation phases and patterns indicating recombinant protein production in various E. coli host strains.Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology.Purification of inclusion bodies using PEG precipitation under denaturing conditions to produce recombinant therapeutic proteins from Escherichia coli.Elucidation of auxotrophic deficiencies of Bacillus pumilus DSM 18097 to develop a defined minimal medium.
P2860

Exchange of single amino acids at different positions of a recombinant protein affects metabolic burden in Escherichia coli.

Item
http://www.wikidata.org/entity/Q43120768
description
2015 nî lūn-bûn
@nan
2015年の論文
@ja
2015年学术文章
@wuu
2015年学术文章
@zh
2015年学术文章
@zh-cn
2015年学术文章
@zh-hans
2015年学术文章
@zh-my
2015年学术文章
@zh-sg
2015年學術文章
@yue
2015年學術文章
@zh-hant
name
Exchange of single amino acids ...... ic burden in Escherichia coli.
@en
Exchange of single amino acids ...... ic burden in Escherichia coli.
@nl
type
label
Exchange of single amino acids ...... ic burden in Escherichia coli.
@en
Exchange of single amino acids ...... ic burden in Escherichia coli.
@nl
prefLabel
Exchange of single amino acids ...... ic burden in Escherichia coli.
@en
Exchange of single amino acids ...... ic burden in Escherichia coli.
@nl
P1433
P1476
P2093
P2860
P2888
P304
P31
P356
P478
P50
P577
P6179
P698
P932
P356
P1433
P1476
Exchange of single amino acids ...... ic burden in Escherichia coli.
@en
P2093
P2860
P2888
P304
P31
P356
P478
P50
P577
P6179
P698
P932