about
Structure-guided evolution of cyan fluorescent proteins towards a quantum yield of 93%.Spatiotemporal analysis of RhoA/B/C activation in primary human endothelial cellsF-actin-rich contractile endothelial pores prevent vascular leakage during leukocyte diapedesis through local RhoA signallingA mTurquoise-based cAMP sensor for both FLIM and ratiometric read-out has improved dynamic rangeStructure of a fluorescent protein fromAequorea victoriabearing the obligate-monomer mutation A206KSignaling efficiency of Gαq through its effectors p63RhoGEF and GEFT depends on their subcellular locationPractical and reliable FRET/FLIM pair of fluorescent proteins.siFLIM: single-image frequency-domain FLIM provides fast and photon-efficient lifetime data.Sensitive detection of p65 homodimers using red-shifted and fluorescent protein-based FRET couples.Quantitative lifetime unmixing of multiexponentially decaying fluorophores using single-frequency fluorescence lifetime imaging microscopy.The anti-apoptotic activity associated with phosphatidylinositol transfer protein alpha activates the MAPK and Akt/PKB pathway.Stochastic and reversible assembly of a multiprotein DNA repair complex ensures accurate target site recognition and efficient repair.Real-time visualization of heterotrimeric G protein Gq activation in living cellsQuantitative co-expression of proteins at the single cell level--application to a multimeric FRET sensor.Dynamic in vivo interaction of DDB2 E3 ubiquitin ligase with UV-damaged DNA is independent of damage-recognition protein XPC.Optimization of fluorescent proteins.Fourth-generation epac-based FRET sensors for cAMP feature exceptional brightness, photostability and dynamic range: characterization of dedicated sensors for FLIM, for ratiometry and with high affinityA New Generation of FRET Sensors for Robust Measurement of Gαi1, Gαi2 and Gαi3 Activation Kinetics in Single CellsA Perspective on Studying G-Protein-Coupled Receptor Signaling with Resonance Energy Transfer Biosensors in Living Organisms.Plasma membrane restricted RhoGEF activity is sufficient for RhoA-mediated actin polymerization.Multiparameter imaging for the analysis of intracellular signaling.An introduction to fluorescence imaging techniques geared towards biosensor applications.Development of FRET biosensors for mammalian and plant systems.Rapid colorimetric quantification of lipo-chitooligosaccharides from Mesorhizobium loti and Sinorhizobium meliloti.Molecular perturbation strategies to examine spatiotemporal features of Rho GEF and Rho GTPase activity in living cells.Tetraspanin microdomains control localized protein kinase C signaling in B cells.Effect of fixation procedures on the fluorescence lifetimes of Aequorea victoria derived fluorescent proteins.Transfection of cells with DNA encoding a visible fluorescent protein-tagged lipid-binding domain.Uniform cAMP stimulation of Dictyostelium cells induces localized patches of signal transduction and pseudopodia.Domain analysis of the Nematostella vectensis SNAIL ortholog reveals unique nucleolar localization that depends on the zinc-finger domains.A local VE-cadherin and Trio-based signaling complex stabilizes endothelial junctions through Rac1.Characterization of a spectrally diverse set of fluorescent proteins as FRET acceptors for mTurquoise2.PKC gamma mutations in spinocerebellar ataxia type 14 affect C1 domain accessibility and kinase activity leading to aberrant MAPK signaling.SCA14 mutation V138E leads to partly unfolded PKCγ associated with an exposed C-terminus, altered kinetics, phosphorylation and enhanced insolubilization.Fluorescence resonance energy transfer imaging of PKC signalling in living cells using genetically encoded fluorescent probes.Improved green and blue fluorescent proteins for expression in bacteria and mammalian cells.Cyan and yellow super fluorescent proteins with improved brightness, protein folding, and FRET Förster radius.Bright monomeric red fluorescent protein with an extended fluorescence lifetime.Quantitative Single-Cell Analysis of Signaling Pathways Activated Immediately Downstream of Histamine Receptor Subtypes.PLCβ isoforms differ in their subcellular location and their CT-domain dependent interaction with Gαq.
P50
Q24606976-991C31A1-CBE1-4B69-9804-551CC0917D0DQ27317056-18C28342-CC88-487F-B621-48BFFEC904EBQ27334439-F6E271A7-283A-4808-8194-469000D35CC5Q27345658-90D70BBF-AE8A-42EC-ADBD-77BB7504A20DQ27671237-214B137E-97D2-4BE6-A502-ECB4CD314EE3Q28673501-7E570498-EBF2-4B37-998D-AA76ACBEBBD5Q30487440-0B41C866-680B-4011-86D7-14C4A64F0FCCQ31079596-8218AFE2-B65C-4534-80B0-A23CADC1002EQ33302110-2807B50E-8CDF-4196-A01F-864F45E32A99Q33325120-13EAA5EC-5EA3-4845-9EC2-C5C730B64F6CQ33337580-E96CBAD7-1801-46BD-8FB0-E6B9A8E3E0AEQ33840056-C9708248-EA33-4021-897C-DF7F862794F8Q33914273-553BBBCE-351F-4FB4-85CE-587FFF00CD4BQ34082758-4702F191-F592-4A12-8D9A-852F861D3CA0Q34652298-29BA53EE-984F-4F6C-BB1B-2576861CC6FAQ35012814-C8927FE3-A74C-4D72-A67A-4DE7464BDBB1Q35602122-D9F24970-0059-488C-8DEE-250043388252Q35901295-4942C2C5-ADDC-458C-B39F-37312DDCAF8CQ35999782-6D91CCA5-6A94-4FB9-8FE4-513BB918FAB4Q36119815-B78E6DB6-DB47-4013-9CC3-DE8FE7FE9F80Q36190680-71093EA1-47D7-4C09-95DA-88FA321F20F6Q38139887-0BC7C244-2903-4937-A514-7ED03A5455D0Q38171304-60570D34-3EDF-400B-9FAA-A4A11D35C9CFQ38362874-82A2979A-FE3F-4577-9DFF-F1DE67014179Q38682208-0A235488-A4B1-43EE-801E-BB5A8D1A6CFAQ38795253-2B1AAFAB-343D-45C9-90E0-A63690FAA2EAQ38960690-FD02D789-D2DD-493D-9B76-A2631A37970DQ39677735-F3EBA53F-E10E-40E5-A728-3718C0B27030Q40286962-E463B9CB-6051-41D5-BC5D-C9EEF4BCB82AQ40719959-BE8A36B3-AE57-452D-9059-CB9DC9797467Q40791540-111087D3-9E88-4CF0-B320-F589F165C923Q41696655-B9CE052D-C3D7-48C6-B558-0D0B64ACC286Q42660284-BFE5D2F6-3158-4D93-BF57-9A58F0B73ABEQ43905253-31B1249E-B876-4ADB-BA0A-D6FB1FE9353AQ45766627-DC69D3C2-C679-4BB1-984F-FCC23EC3EBF3Q48081270-F4C9BCD7-88C4-448A-BC9E-977B20749F1FQ48087580-A789AE3C-D1DC-4240-8EBA-22F35BADBFF7Q50691400-F1B8EDF9-2DA9-4DFD-849E-E28D4924889DQ51673015-EB9A2E9E-64CD-4180-B1D2-42103D7B1B9CQ53359504-097D687C-6729-4737-AEEB-312F82F82E85
P50
description
hulumtues
@sq
researcher
@en
wetenschapper
@nl
հետազոտող
@hy
name
Joachim Goedhart
@ast
Joachim Goedhart
@br
Joachim Goedhart
@co
Joachim Goedhart
@cs
Joachim Goedhart
@da
Joachim Goedhart
@de
Joachim Goedhart
@en
Joachim Goedhart
@es
Joachim Goedhart
@fr
Joachim Goedhart
@id
type
label
Joachim Goedhart
@ast
Joachim Goedhart
@br
Joachim Goedhart
@co
Joachim Goedhart
@cs
Joachim Goedhart
@da
Joachim Goedhart
@de
Joachim Goedhart
@en
Joachim Goedhart
@es
Joachim Goedhart
@fr
Joachim Goedhart
@id
altLabel
J. Goedhart
@nl
prefLabel
Joachim Goedhart
@ast
Joachim Goedhart
@br
Joachim Goedhart
@co
Joachim Goedhart
@cs
Joachim Goedhart
@da
Joachim Goedhart
@de
Joachim Goedhart
@en
Joachim Goedhart
@es
Joachim Goedhart
@fr
Joachim Goedhart
@id
P106
P2002
joachimgoedhart
P21
P31
P496
0000-0002-0630-3825
P734
P735
P7449
PRS1311263