A simple high-yielding process for transient gene expression in CHO cells.
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Recent advances in mammalian protein productionFlexible antibodies with nonprotein hinges.The use of filler DNA for improved transfection and reduced DNA needs in transient gene expression with CHO and HEK cellsEnhanced transient recombinant protein production in CHO cells through the co-transfection of the product gene with Bcl-xL.N-isopropylacrylamide-modified polyethylenimines as effective gene carriers.Multi-host expression system for recombinant production of challenging proteins.Glycosylation profiles determine extravasation and disease-targeting properties of armed antibodiesBridging the gap: facilities and technologies for development of early stage therapeutic mAb candidatesAntigen generation and display in therapeutic antibody drug discovery -- a neglected but critical player.New Mammalian Expression Systems.Production process reproducibility and product quality consistency of transient gene expression in HEK293 cells with anti-PD1 antibody as the model protein.Tetrahydrofolate increases suspension growth of dihydrofolate reductase-deficient chinese hamster ovary DG44 cells in chemically defined media.CHO-S antibody titers >1 gram/liter using flow electroporation-mediated transient gene expression followed by rapid migration to high-yield stable cell lines.A high cell density transient transfection system for therapeutic protein expression based on a CHO GS-knockout cell line: process development and product quality assessment.High-throughput mAb expression and purification platform based on transient CHO.Effect of Peptone Feeding on Transient Gene Expression Process in CHO DG44.A high-yielding CHO transient system: coexpression of genes encoding EBNA-1 and GS enhances transient protein expression.Model-directed engineering of "difficult-to-express" monoclonal antibody production by Chinese hamster ovary cells.Use of an anti-apoptotic CHO cell line for transient gene expression.A CHO cell line engineered to express XBP1 and ERO1-Lα has increased levels of transient protein expression.Transient recombinant protein expression in a human amniocyte cell line: the CAP-T® cell system.Reduced glutamine concentration improves protein production in growth-arrested CHO-DG44 and HEK-293E cells.A simple, rapid method for evaluation of transfection efficiency based on fluorescent dye.NF-κB, CRE and YY1 elements are key functional regulators of CMV promoter-driven transient gene expression in CHO cells.Polymer-mediated flocculation of transient CHO cultures as a simple, high throughput method to facilitate antibody discovery.Advancements in mammalian cell transient gene expression (TGE) technology for accelerated production of biologics.Poly(ethyleneimine)-mediated large-scale transient gene expression: influence of molecular weight, polydispersity and N-propionyl groups.Trans-acting oligodeoxythymidine phosphorothioate triester reagents for transient transfection optimized and facilitated by a high-throughput microbioreactor system.Trends in Upstream and Downstream Process Development for Antibody Manufacturing.Potency-matched Dual Cytokine-Antibody Fusion Proteins for Cancer Therapy.Enhanced plasmid DNA utilization in transiently transfected CHO-DG44 cells in the presence of polar solvents.Large-Scale Transient Transfection of Chinese Hamster Ovary Cells in Suspension.Integrated cell and process engineering for improved transient production of a "difficult-to-express" fusion protein by CHO cells.Transcriptional and post-transcriptional limitations of high-yielding, PEI-mediated transient transfection with CHO and HEK-293E cells.Hyperbranched polylysine: a versatile, biodegradable transfection agent for the production of recombinant proteins by transient gene expression and the transfection of primary cells.Role of non-specific DNA in reducing coding DNA requirement for transient gene expression with CHO and HEK-293E cells.A novel dual-cytokine-antibody fusion protein for the treatment of CD38-positive malignancies
P2860
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P2860
A simple high-yielding process for transient gene expression in CHO cells.
description
2011 nî lūn-bûn
@nan
2011年の論文
@ja
2011年学术文章
@wuu
2011年学术文章
@zh
2011年学术文章
@zh-cn
2011年学术文章
@zh-hans
2011年学术文章
@zh-my
2011年学术文章
@zh-sg
2011年學術文章
@yue
2011年學術文章
@zh-hant
name
A simple high-yielding process for transient gene expression in CHO cells.
@en
A simple high-yielding process for transient gene expression in CHO cells.
@nl
type
label
A simple high-yielding process for transient gene expression in CHO cells.
@en
A simple high-yielding process for transient gene expression in CHO cells.
@nl
prefLabel
A simple high-yielding process for transient gene expression in CHO cells.
@en
A simple high-yielding process for transient gene expression in CHO cells.
@nl
P2093
P1476
A simple high-yielding process for transient gene expression in CHO cells
@en
P2093
David L Hacker
Divor Kiseljak
Florian M Wurm
Lucia Baldi
P356
10.1016/J.JBIOTEC.2011.03.001
P577
2011-03-15T00:00:00Z