General method for sequence-independent site-directed chimeragenesis.
about
Structure-guided recombination creates an artificial family of cytochromes P450.Directed evolution of protein enzymes using nonhomologous random recombinationExploring protein fitness landscapes by directed evolutionA novel high-throughput (HTP) cloning strategy for site-directed designed chimeragenesis and mutation using the Gateway cloning system.To get what we aim for--progress in diversity generation methodsReprogramming cells with synthetic proteinsDirected evolution: tailoring biocatalysts for industrial applicationsChemical and biochemical strategies for the randomization of protein encoding DNA sequences: library construction methods for directed evolution.Evolution of highly active enzymes by homology-independent recombination.FamClash: a method for ranking the activity of engineered enzymes.Laboratory-directed protein evolutionIPRO: an iterative computational protein library redesign and optimization procedureDirected evolution: an approach to engineer enzymes.STAR: predicting recombination sites from amino acid sequence.Computationally designed libraries of fluorescent proteins evaluated by preservation and diversity of function.Motif programming: a microgene-based method for creating synthetic proteins containing multiple functional motifsSteering directed protein evolution: strategies to manage combinatorial complexity of mutant libraries.Combinatorial recombination of gene fragments to construct a library of chimeras.Random dissection to select for protein split sites and its application in protein fragment complementationGolden gate shuffling: a one-pot DNA shuffling method based on type IIs restriction enzymes.Optimization of combinatorial mutagenesis.Enhancing the efficiency of directed evolution in focused enzyme libraries by the adaptive substituent reordering algorithm.A diverse set of family 48 bacterial glycoside hydrolase cellulases created by structure-guided recombination.Chimeric β-lactamases: global conservation of parental function and fast time-scale dynamics with increased slow motionsFunctionally diverging molecular quasi-species evolve by crossing two enzymesCombinatorial engineering of 1-deoxy-D-xylulose 5-phosphate pathway using cross-lapping in vitro assembly (CLIVA) method.Library generation by gene shuffling.Directed Evolution of Enzymes for Industrial Biocatalysis.Exploring the potential of xenobiotic-metabolising enzymes as biocatalysts: evolving designer catalysts from polyfunctional cytochrome P450 enzymes.Enzyme engineering: A synthetic biology approach for more effective library generation and automated high-throughput screeningConstructing de novo biosynthetic pathways for chemical synthesis inside living cells.Methods for the directed evolution of proteins.A novel approach to the generation of seamless constructs for plant transformationSynthetic biology of modular proteins.Principles for computational design of binding antibodies.Combinatorial recombination of gene fragments to construct a library of chimeras
P2860
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P2860
General method for sequence-independent site-directed chimeragenesis.
description
2003 nî lūn-bûn
@nan
2003 թուականի Յուլիսին հրատարակուած գիտական յօդուած
@hyw
2003 թվականի հուլիսին հրատարակված գիտական հոդված
@hy
2003年の論文
@ja
2003年論文
@yue
2003年論文
@zh-hant
2003年論文
@zh-hk
2003年論文
@zh-mo
2003年論文
@zh-tw
2003年论文
@wuu
name
General method for sequence-independent site-directed chimeragenesis.
@ast
General method for sequence-independent site-directed chimeragenesis.
@en
type
label
General method for sequence-independent site-directed chimeragenesis.
@ast
General method for sequence-independent site-directed chimeragenesis.
@en
prefLabel
General method for sequence-independent site-directed chimeragenesis.
@ast
General method for sequence-independent site-directed chimeragenesis.
@en
P1476
General method for sequence-independent site-directed chimeragenesis.
@en
P2093
Kaori Hiraga
P304
P356
10.1016/S0022-2836(03)00590-4
P407
P577
2003-07-01T00:00:00Z