Why high-error-rate random mutagenesis libraries are enriched in functional and improved proteins.
about
Evolution favors protein mutational robustness in sufficiently large populationsProtein stability promotes evolvabilityExploring protein fitness landscapes by directed evolutionDirected evolution strategies for improved enzymatic performanceTo get what we aim for--progress in diversity generation methodsEstablishing catalytic activity on an artificial (βα)8-barrel protein designed from identical half-barrelsHigh throughput mutagenesis for identification of residues regulating human prostacyclin (hIP) receptor expression and functionA framework for evolutionary systems biologyLethal mutagenesis of bacteriaHistorical contingency and its biophysical basis in glucocorticoid receptor evolution.Enzyme improvement in the absence of structural knowledge: a novel statistical approach.Stabilization of a metabolic enzyme by library selection in Thermus thermophilus.Engineering and rapid selection of a low-affinity glucose/galactose-binding protein for a glucose biosensorAssessing the potential of mutational strategies to elicit new phenotypes in industrial strains.A study in molecular contingency: glutamine phosphoribosylpyrophosphate amidotransferase is a promiscuous and evolvable phosphoribosylanthranilate isomerase.Engineering antibody fragments to fold in the absence of disulfide bonds.Assessing the potential of an induced-mutation strategy for avermectin overproducersA novel constructed SPT15 mutagenesis library of Saccharomyces cerevisiae by using gTME technique for enhanced ethanol productionEngineering a large protein by combined rational and random approaches: stabilizing the Clostridium thermocellum cellobiose phosphorylase.Predicting the tolerance of proteins to random amino acid substitution.Lessons from diversity of directed evolution experiments by an analysis of 3,000 mutations.SpeedyGenes: an improved gene synthesis method for the efficient production of error-corrected, synthetic protein libraries for directed evolution.Principles and application of antibody libraries for infectious diseases.Structure-based design of combinatorial mutagenesis librariesAn in vitro compartmentalization-based method for the selection of bond-forming enzymes from large librariesFacile Construction of Random Gene Mutagenesis Library for Directed Evolution Without the Use of Restriction Enzyme in Escherichia coli.Sort-Seq Approach to Engineering a Formaldehyde-Inducible Promoter for Dynamically Regulated Escherichia coli Growth on Methanol.Casting epPCR (cepPCR): A simple random mutagenesis method to generate high quality mutant libraries.A population-based experimental model for protein evolution: effects of mutation rate and selection stringency on evolutionary outcomes.Synonymous genes explore different evolutionary landscapes.Improved activity of a thermophilic cellulase, Cel5A, from Thermotoga maritima on ionic liquid pretreated switchgrass.Combinatorial approaches for inverse metabolic engineering applications.Synthetic biology for the directed evolution of protein biocatalysts: navigating sequence space intelligently.GLUE-IT and PEDEL-AA: new programmes for analyzing protein diversity in randomized librariesBacterial Phytochromes, Cyanobacteriochromes and Allophycocyanins as a Source of Near-Infrared Fluorescent Probes.Fitness loss and library size determination in saturation mutagenesisThe optimal burst of mutation to create a phenotype.The intelligent design of evolution.Functional mutations in and characterization of VHH against Helicobacter pylori urease.Rapid evolution of arginine deiminase for improved anti-tumor activity.
P2860
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P2860
Why high-error-rate random mutagenesis libraries are enriched in functional and improved proteins.
description
2005 nî lūn-bûn
@nan
2005 թուականի Յուլիսին հրատարակուած գիտական յօդուած
@hyw
2005 թվականի հուլիսին հրատարակված գիտական հոդված
@hy
2005年の論文
@ja
2005年論文
@yue
2005年論文
@zh-hant
2005年論文
@zh-hk
2005年論文
@zh-mo
2005年論文
@zh-tw
2005年论文
@wuu
name
Why high-error-rate random mut ...... ctional and improved proteins.
@ast
Why high-error-rate random mut ...... ctional and improved proteins.
@en
type
label
Why high-error-rate random mut ...... ctional and improved proteins.
@ast
Why high-error-rate random mut ...... ctional and improved proteins.
@en
prefLabel
Why high-error-rate random mut ...... ctional and improved proteins.
@ast
Why high-error-rate random mut ...... ctional and improved proteins.
@en
P1476
Why high-error-rate random mut ...... ctional and improved proteins.
@en
P2093
Brent L Iverson
George Georgiou
P304
P356
10.1016/J.JMB.2005.05.023
P577
2005-07-01T00:00:00Z
P5875
P698
P818
q-bio/0411041