Cryogenic electron microscopy
Cryogenic electron microscopy (cryo-EM) is an electron microscopy (EM) technique applied on samples cooled to cryogenic temperatures and embedded in an environment of vitreous water. An aqueous sample solution is applied to a grid-mesh and plunge-frozen in liquid ethane or a mixture of liquid ethane and propane. While development of the technique began in the 1970s, recent advances in detector technology and software algorithms have allowed for the determination of biomolecular structures at near-atomic resolution. This has attracted wide attention to the approach as an alternative to X-ray crystallography or NMR spectroscopy for macromolecular structure determination without the need for crystallization.
2017 in science43S preinitiation complex5S ribosomal RNAATP synthaseActinAction potentialAlpha-synucleinAlphaFoldAlphachrysovirusAlphavirusAmorphous iceAmphipolsAmyloidApoptosomeAquareovirusAsgard (archaea)Batai orthobunyavirusBik Kwoon TyeBiological data visualizationBiomolecular structureBiophysical SocietyBiophysical chemistryBocaparvovirusC9orf72CCP4 (file format)CD3 (immunology)Cafeteria roenbergensis virusCalcium-dependent chloride channelCarboxysomeChikashi ToyoshimaChikungunyaChlorosomeChrysoviridaeCluster of Excellence Frankfurt Macromolecular ComplexesContrast transfer functionCoolantCryoCryo-EMCryo-Electron MicroscopyCryo-electron microscopy
Link from a Wikipage to another Wikipage
primaryTopic
Cryogenic electron microscopy
Cryogenic electron microscopy (cryo-EM) is an electron microscopy (EM) technique applied on samples cooled to cryogenic temperatures and embedded in an environment of vitreous water. An aqueous sample solution is applied to a grid-mesh and plunge-frozen in liquid ethane or a mixture of liquid ethane and propane. While development of the technique began in the 1970s, recent advances in detector technology and software algorithms have allowed for the determination of biomolecular structures at near-atomic resolution. This has attracted wide attention to the approach as an alternative to X-ray crystallography or NMR spectroscopy for macromolecular structure determination without the need for crystallization.
has abstract
Crio-microscopia eletrónica (p ...... nar sua qualidade prejudicada.
@pt
Cryogenic electron microscopy ...... "Method of the Year" in 2016.
@en
Die Kryoelektronenmikroskopie ...... (≲ −150 °C) untersucht werden.
@de
Kryoelektronová mikroskopie (a ...... lo a nedojde k jeho deformaci.
@cs
La criomicroscopía electrónica ...... permite mejorar la resolución.
@es
La cryo-microscopie électroniq ...... les (3D) d’objets biologiques.
@fr
La microscopia crioelettronica ...... ottenute a varie angolazioni.
@it
Mikroskopia krioelektronowa (C ...... się znacznie ostrzejszy obraz.
@pl
Криоэлектронная микроскопия (к ...... соким разрешением в растворе».
@ru
Кріоелектронна мікроскопія (аб ...... здільною здатністю в розчині».
@uk
Wikipage page ID
56,452,747
page length (characters) of wiki page
Wikipage revision ID
1,022,455,620
Link from a Wikipage to another Wikipage
wikiPageUsesTemplate
comment
Crio-microscopia eletrónica (p ...... a e em sua conformação nativa.
@pt
Cryogenic electron microscopy ...... the need for crystallization.
@en
Die Kryoelektronenmikroskopie ...... (≲ −150 °C) untersucht werden.
@de
Kryoelektronová mikroskopie (a ...... lo a nedojde k jeho deformaci.
@cs
La criomicroscopía electrónica ...... nativos a resolución atómica.
@es
La cryo-microscopie électroniq ...... la structure des échantillons.
@fr
La microscopia crioelettronica ...... oter osservare le macromolecol
@it
Mikroskopia krioelektronowa (C ...... zenia wizualizacji cząsteczki:
@pl
Криоэлектронная микроскопия (к ...... им конформационным изменениям.
@ru
Кріоелектронна мікроскопія (аб ...... за кріогенними температурами.
@uk
label
Crio-microscopia eletrónica
@pt
Criomicroscopía electrónica
@es
Cryo-microscopie électronique
@fr
Cryogenic electron microscopy
@en
Kryoelektronenmikroskopie
@de
Kryoelektronmikroskopi
@sv
Kryoelektronová mikroskopie
@cs
Microscopia crioelettronica
@it
Mikroskopia krioelektronowa
@pl
Криоэлектронная микроскопия
@ru