Redesigning the substrate specificity of an enzyme by cumulative effects of the mutations of non-active site residues
about
The "Transport Specificity Ratio": a structure-function tool to search the protein fold for loci that control transition state stability in membrane transport catalysisA structural view of evolutionary divergenceMutations of penicillin acylase residue B71 extend substrate specificity by decreasing steric constraints for substrate bindingThe molecular basis of glyphosate resistance by an optimized microbial acetyltransferaseDirected Evolution and Structural Characterization of a Simvastatin SynthaseStructural Insights into Substrate Specificity in Variants of N-Acetylneuraminic Acid Lyase Produced by Directed EvolutionBiocatalytic and structural properties of a highly engineered halohydrin dehalogenaseIn vitro Engineering of Novel Bioactivity in the Natural EnzymesFrom cofactor to enzymes. The molecular evolution of pyridoxal-5'-phosphate-dependent enzymesHeterotachy, an important process of protein evolutionReconstruction of mycobacterial dehalogenase Rv2579 by cumulative mutagenesis of haloalkane dehalogenase LinBMechanistic approaches to the study of evolution: the functional synthesisInvestigating and Engineering Enzymes by Genetic Selection.Generation of a broad esterolytic subtilisin using combined molecular evolution and periplasmic expression.Quantitative analysis of the effect of the mutation frequency on the affinity maturation of single chain Fv antibodies.Transmutation of human glutathione transferase A2-2 with peroxidase activity into an efficient steroid isomerase.How protein stability and new functions trade offMolecular evolution of B6 enzymes: binding of pyridoxal-5'-phosphate and Lys41Arg substitution turn ribonuclease A into a model B6 protoenzyme.Induced allostery in the directed evolution of an enantioselective Baeyer-Villiger monooxygenase.Single-mutation fitness landscapes for an enzyme on multiple substrates reveal specificity is globally encodedReduced amino acid specificity of mammalian tyrosyl-tRNA synthetase is associated with elevated mistranslation of Tyr codons.Directed evolution of D-sialic acid aldolase to L-3-deoxy-manno-2-octulosonic acid (L-KDO) aldolase.NMR and bioinformatics discovery of exosites that tune metalloelastase specificity for solubilized elastin and collagen triple helices.Evidence for niche partitioning revealed by the distribution of sulfur oxidation genes collected from areas of a terrestrial sulfidic spring with differing geochemical conditions.Discovery of superior enzymes by directed molecular evolution.Structural plasticity of an aminoacyl-tRNA synthetase active siteDirected evolution of the substrate specificity of dialkylglycine decarboxylaseRedesign of substrate-selectivity determining modules of glutathione transferase A1-1 installs high catalytic efficiency with toxic alkenal products of lipid peroxidation.Structure-function analysis from the outside in: long-range tertiary contacts in RNA exhibit distinct catalytic roles.Reengineering the glutathione S-transferase scaffold: a rational design strategy pays off.Directed evolution to improve the catalytic efficiency of urate oxidase from Bacillus subtilis.Evolving protein functional diversity in new genes of DrosophilaExperimental and computational mutagenesis to investigate the positioning of a general base within an enzyme active site.Synthetic biology for the directed evolution of protein biocatalysts: navigating sequence space intelligently.Prediction of distal residue participation in enzyme catalysis.Modulation of individual steps in group I intron catalysis by a peripheral metal ion.A change of the metal-specific activity of a cambialistic superoxide dismutase from Porphyromonas gingivalis by a double mutation of Gln-70 to Gly and Ala-142 to Gln.Structural determinants in domain II of human glutathione transferase M2-2 govern the characteristic activities with aminochrome, 2-cyano-1,3-dimethyl-1-nitrosoguanidine, and 1,2-dichloro-4-nitrobenzene.Elimination of 2-keto-3-deoxy-D-glycero-D-galacto-nonulosonic acid 9-phosphate synthase activity from human N-acetylneuraminic acid 9-phosphate synthase by a single mutation.Tightening of active site interactions en route to the transition state revealed by single-atom substitution in the guanosine-binding site of the Tetrahymena group I ribozyme.
P2860
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P2860
Redesigning the substrate specificity of an enzyme by cumulative effects of the mutations of non-active site residues
description
1999 nî lūn-bûn
@nan
1999 թուականի Յունուարին հրատարակուած գիտական յօդուած
@hyw
1999 թվականի հունվարին հրատարակված գիտական հոդված
@hy
1999年の論文
@ja
1999年論文
@yue
1999年論文
@zh-hant
1999年論文
@zh-hk
1999年論文
@zh-mo
1999年論文
@zh-tw
1999年论文
@wuu
name
Redesigning the substrate spec ...... ns of non-active site residues
@ast
Redesigning the substrate spec ...... ns of non-active site residues
@en
Redesigning the substrate spec ...... ns of non-active site residues
@nl
type
label
Redesigning the substrate spec ...... ns of non-active site residues
@ast
Redesigning the substrate spec ...... ns of non-active site residues
@en
Redesigning the substrate spec ...... ns of non-active site residues
@nl
prefLabel
Redesigning the substrate spec ...... ns of non-active site residues
@ast
Redesigning the substrate spec ...... ns of non-active site residues
@en
Redesigning the substrate spec ...... ns of non-active site residues
@nl
P2093
P2860
P3181
P356
P1476
Redesigning the substrate spec ...... ns of non-active site residues
@en
P2093
P2860
P304
P3181
P356
10.1074/JBC.274.4.2344
P407
P577
1999-01-22T00:00:00Z