Investigating and Engineering Enzymes by Genetic Selection.
about
Simultaneous optimization of enzyme activity and quaternary structure by directed evolutionDNA-templated organic synthesis and selection of a library of macrocyclesExamining protein surface structure in highly conserved sequence variants with mass spectrometry.Chemical and biochemical strategies for the randomization of protein encoding DNA sequences: library construction methods for directed evolution.Assembly of designed oligonucleotides as an efficient method for gene recombination: a new tool in directed evolution.Combinatorial approaches to protein stability and structure.Mechanistic insights into the isochorismate pyruvate lyase activity of the catalytically promiscuous PchB from combinatorial mutagenesis and selection.Error-prone rolling circle amplification: the simplest random mutagenesis protocol.In vivo selection for the directed evolution of L-rhamnulose aldolase from L-rhamnulose-1-phosphate aldolase (RhaD).Metabolic engineering of a genetic selection system with tunable stringencyRevealing biases inherent in recombination protocols.In vitro selection of a DNA-templated small-molecule library reveals a class of macrocyclic kinase inhibitorsRevisiting the lipase from Pseudomonas aeruginosa: directed evolution of substrate acceptance and enantioselectivity using iterative saturation mutagenesis.A self-replicating ligase ribozyme.Assessing directed evolution methods for the generation of biosynthetic enzymes with potential in drug biosynthesis.Deciphering enzymes. Genetic selection as a probe of structure and mechanism.A growth- and bioluminescence-based bioreporter for the in vivo detection of novel biocatalysts.Selection strategies for improved biocatalysts.Directed evolution of ligand dependence: small-molecule-activated protein splicing.An enzymatic molten globule: efficient coupling of folding and catalysis.Controlling the enantioselectivity of enzymes by directed evolution: practical and theoretical ramifications.Synthetic biology for the directed evolution of protein biocatalysts: navigating sequence space intelligently.Directed evolution of a ring-cleaving dioxygenase for polychlorinated biphenyl degradation.Biosynthetic engineering of nonribosomal peptide synthetases.The role of side chain entropy and mutual information for improving the de novo design of Kemp eliminases KE07 and KE70.The adrenaline test for enzymes.A platform for high-throughput screening of DNA-encoded catalyst libraries in organic solvents.Improved thermostability of lipase B from Candida antarctica by directed evolution and display on yeast surface.Classifying enzymes from selectivity fingerprints.Archimedes' principle for characterisation of recombinant whole cell biocatalysts.Emergence of a catalytic tetrad during evolution of a highly active artificial aldolase.Rapid and direct spectrophotometric method for kinetics studies and routine assay of peroxidase based on aniline diazo substrates.
P2860
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P2860
Investigating and Engineering Enzymes by Genetic Selection.
description
2001 nî lūn-bûn
@nan
2001 թուականի Սեպտեմբերին հրատարակուած գիտական յօդուած
@hyw
2001 թվականի սեպտեմբերին հրատարակված գիտական հոդված
@hy
2001年の論文
@ja
2001年論文
@yue
2001年論文
@zh-hant
2001年論文
@zh-hk
2001年論文
@zh-mo
2001年論文
@zh-tw
2001年论文
@wuu
name
Investigating and Engineering Enzymes by Genetic Selection.
@ast
Investigating and Engineering Enzymes by Genetic Selection.
@en
type
label
Investigating and Engineering Enzymes by Genetic Selection.
@ast
Investigating and Engineering Enzymes by Genetic Selection.
@en
prefLabel
Investigating and Engineering Enzymes by Genetic Selection.
@ast
Investigating and Engineering Enzymes by Genetic Selection.
@en
P2093
P2860
P1433
P1476
Investigating and Engineering Enzymes by Genetic Selection.
@en
P2093
Donald Hilvert
Peter Kast
Sean V. Taylor
P2860
P304
P356
10.1002/1521-3773(20010917)40:18<3310::AID-ANIE3310>3.0.CO;2-P
P577
2001-09-01T00:00:00Z