Minimalist active-site redesign: teaching old enzymes new tricks
about
Protein design: toward functional metalloenzymesAllosteric activation transitions in enzymes and biomolecular motors: insights from atomistic and coarse-grained simulationsEstablishing wild-type levels of catalytic activity on natural and artificial ( )8-barrel protein scaffoldsAn efficient, multiply promiscuous hydrolase in the alkaline phosphatase superfamilyInterconversion of the Specificities of Human Lysosomal Enzymes Associated with Fabry and Schindler DiseasesComputational redesign of a mononuclear zinc metalloenzyme for organophosphate hydrolysisA Single Mutation in a Regulatory Protein Produces Evolvable Allosterically Regulated Catalyst of Nonnatural ReactionEstablishing catalytic activity on an artificial (βα)8-barrel protein designed from identical half-barrelsErgothioneine biosynthetic methyltransferase EgtD reveals the structural basis of aromatic amino acid betaine biosynthesisA Promiscuous De Novo Retro-Aldolase Catalyzes Asymmetric Michael Additions via Schiff Base IntermediatesTuning and Switching Enantioselectivity of Asymmetric Carboligation in an Enzyme through Mutational Analysis of a Single Hot SpotCombining a Clostridial enzyme exhibiting unusual active site plasticity with a remarkably facile sigmatropic rearrangement: rapid, stereocontrolled entry into densely functionalized fluorinated phosphonates for chemical biologyComputational protein engineering: bridging the gap between rational design and laboratory evolution.Saturation mutagenesis of Asn152 reveals a substrate selectivity switch in P99 cephalosporinaseTransCent: computational enzyme design by transferring active sites and considering constraints relevant for catalysis.Consensus protein design without phylogenetic biasProtein thermostability calculations using alchemical free energy simulationsToward accurate screening in computer-aided enzyme design.Rational Engineering of a Cold-Adapted α-Amylase from the Antarctic Ciliate Euplotes focardii for Simultaneous Improvement of Thermostability and Catalytic Activity.(R,S)-2-chlorophenoxyl pyrazolides as novel substrates for improving lipase-catalyzed hydrolytic resolution.Exploring challenges in rational enzyme design by simulating the catalysis in artificial kemp eliminase.QM/MM analysis suggests that Alkaline Phosphatase (AP) and nucleotide pyrophosphatase/phosphodiesterase slightly tighten the transition state for phosphate diester hydrolysis relative to solution: implication for catalytic promiscuity in the AP supeChallenges and advances in validating enzyme design proposals: the case of kemp eliminase catalysisA smart library of epoxide hydrolase variants and the top hits for synthesis of (S)-β-blocker precursors.Catalysis by dihydrofolate reductase and other enzymes arises from electrostatic preorganization, not conformational motions.Earthworm is a versatile and sustainable biocatalyst for organic synthesis.Divergent evolution of a bifunctional de novo proteinComputational strategies for the design of new enzymatic functionsEnantioselective Enzyme-Catalyzed Aziridination Enabled by Active-Site Evolution of a Cytochrome P450Probing the mechanisms for the selectivity and promiscuity of methyl parathion hydrolase.Ultrahigh-throughput-directed enzyme evolution by absorbance-activated droplet sorting (AADS).Specificity Effects of Amino Acid Substitutions in Promiscuous Hydrolases: Context-Dependence of Catalytic Residue Contributions to Local Fitness Landscapes in Nearby Sequence Space.Functional tuning of the catalytic residue pKa in a de novo designed esterase.Computational Studies of Candida Antarctica Lipase B to Test Its Capability as a Starting Point To Redesign New Diels-Alderases.Covalent Immobilization of Bacillus licheniformis γ-Glutamyl Transpeptidase on Aldehyde-Functionalized Magnetic Nanoparticles.Mechanistic insights into the bifunctional non-heme iron oxygenase carbapenem synthase by active site saturation mutagenesisAn engineered protease that cleaves specifically after sulfated tyrosine.The empirical valence bond as an effective strategy for computer-aided enzyme design.Synthetic beta-solenoid proteins with the fragment-free computational design of a beta-hairpin extension.Enzyme (re)design: lessons from natural evolution and computation.
P2860
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P2860
Minimalist active-site redesign: teaching old enzymes new tricks
description
2007 nî lūn-bûn
@nan
2007 թուականին հրատարակուած գիտական յօդուած
@hyw
2007 թվականին հրատարակված գիտական հոդված
@hy
2007年の論文
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2007年論文
@yue
2007年論文
@zh-hant
2007年論文
@zh-hk
2007年論文
@zh-mo
2007年論文
@zh-tw
2007年论文
@wuu
name
Minimalist active-site redesign: teaching old enzymes new tricks
@ast
Minimalist active-site redesign: teaching old enzymes new tricks
@en
Minimalist active-site redesign: teaching old enzymes new tricks
@nl
type
label
Minimalist active-site redesign: teaching old enzymes new tricks
@ast
Minimalist active-site redesign: teaching old enzymes new tricks
@en
Minimalist active-site redesign: teaching old enzymes new tricks
@nl
prefLabel
Minimalist active-site redesign: teaching old enzymes new tricks
@ast
Minimalist active-site redesign: teaching old enzymes new tricks
@en
Minimalist active-site redesign: teaching old enzymes new tricks
@nl
P2093
P3181
P356
P1476
Minimalist active-site redesign: teaching old enzymes new tricks
@en
P2093
Donald Hilvert
Kenneth J Woycechowsky
Miguel D Toscano
P304
P3181
P356
10.1002/ANIE.200604205
P407
P577
2007-01-01T00:00:00Z