Controlling the enantioselectivity of enzymes by directed evolution: practical and theoretical ramifications.
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Towards practical biocatalytic Baeyer-Villiger reactions: applying a thermostable enzyme in the gram-scale synthesis of optically-active lactones in a two-liquid-phase system.Computer aided enzyme design and catalytic conceptsDirected evolution: tailoring biocatalysts for industrial applicationsComputationally mapping sequence space to understand evolutionary protein engineering.Engineering of Bacillus lipase by directed evolution for enhanced thermal stability: effect of isoleucine to threonine mutation at protein surface.Directed evolution strategies for enantiocomplementary haloalkane dehalogenases: from chemical waste to enantiopure building blocks.Multiplex-PCR-based recombination as a novel high-fidelity method for directed evolution.Laboratory-directed protein evolutionDirected evolution of Bacillus subtilis lipase A by use of enantiomeric phosphonate inhibitors: crystal structures and phage display selection.Directed evolution: an approach to engineer enzymes.Incorporating Synthetic Oligonucleotides via Gene Reassembly (ISOR): a versatile tool for generating targeted libraries.Development of innovative pediocin PA-1 by DNA shuffling among class IIa bacteriocinsRevealing biases inherent in recombination protocols.Shedding light on the efficacy of laboratory evolution based on iterative saturation mutagenesis.Revisiting the lipase from Pseudomonas aeruginosa: directed evolution of substrate acceptance and enantioselectivity using iterative saturation mutagenesis.Artificial metalloenzymes based on biotin-avidin technology for the enantioselective reduction of ketones by transfer hydrogenation.Enhancing the efficiency of directed evolution in focused enzyme libraries by the adaptive substituent reordering algorithm.Directed evolution by using iterative saturation mutagenesis based on multiresidue sites.Engineering Giardia lamblia trimethylguanosine synthase (GlaTgs2) to transfer non-natural modifications to the RNA 5'-cap.DNA as a versatile chemical component for catalysis, encoding, and stereocontrolDirected Evolution of Enzymes for Industrial Biocatalysis.DNA and RNA induced enantioselectivity in chemical synthesis.Highly L and D enantioselective variants of horseradish peroxidase discovered by an ultrahigh-throughput selection methodNovel trans-Acting Bacillus subtilis glnA mutations that derepress glnRA expression.How a single-point mutation in horseradish peroxidase markedly enhances enantioselectivity.Facilitating the Evolution of Esterase Activity from a Promiscuous Enzyme (Mhg) with Catalytic Functions of Amide Hydrolysis and Carboxylic Acid Perhydrolysis by Engineering the Substrate Entrance Tunnel.Enzyme (re)design: lessons from natural evolution and computation.Chemoselectivity and the curious reactivity preferences of functional groups.Recent advances in engineering proteins for biocatalysis.Biocatalysts: application and engineering for industrial purposes.Directed evolution of a ring-cleaving dioxygenase for polychlorinated biphenyl degradation.Directed evolution of Pseudomonas aeruginosa lipase for improved amide-hydrolyzing activity.Enzymatic site-selectivity enabled by structure-guided directed evolution.Two-step synthesis of fatty acid ethyl ester from soybean oil catalyzed by Yarrowia lipolytica lipase.Control of stereoselectivity in an enzymatic reaction by backdoor access.Directed evolution of an amine oxidase for the preparative deracemisation of cyclic secondary amines.Rational and Semirational Protein Design.What are the Limitations of Enzymes in Synthetic Organic Chemistry?Characterization and evolution of a metagenome-derived lipase towards enhanced enzyme activity and thermostability.High-throughput screening of activity and enantioselectivity of esterases.
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P2860
Controlling the enantioselectivity of enzymes by directed evolution: practical and theoretical ramifications.
description
article científic
@ca
article scientifique
@fr
articolo scientifico
@it
artigo científico
@pt
bilimsel makale
@tr
scientific article published on 12 April 2004
@en
vedecký článok
@sk
vetenskaplig artikel
@sv
videnskabelig artikel
@da
vědecký článek
@cs
name
Controlling the enantioselecti ...... and theoretical ramifications.
@en
Controlling the enantioselecti ...... and theoretical ramifications.
@nl
type
label
Controlling the enantioselecti ...... and theoretical ramifications.
@en
Controlling the enantioselecti ...... and theoretical ramifications.
@nl
prefLabel
Controlling the enantioselecti ...... and theoretical ramifications.
@en
Controlling the enantioselecti ...... and theoretical ramifications.
@nl
P2860
P356
P1476
Controlling the enantioselecti ...... and theoretical ramifications.
@en
P2093
Manfred T Reetz
P2860
P304
P356
10.1073/PNAS.0306866101
P407
P577
2004-04-12T00:00:00Z