Site-directed, Ligase-Independent Mutagenesis (SLIM): a single-tube methodology approaching 100% efficiency in 4 h.
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MiR-205 silences MED1 in hypoxic primary human trophoblastsCoupling between the DEAD-box RNA helicases Ded1p and eIF4ACrystal Structure and Functional Analysis of JMJD5 Indicate an Alternate Specificity and FunctionPlug-and-Play Pairing via Defined Divalent StreptavidinsXanthomonins I-III: a new class of lasso peptides with a seven-residue macrolactam ringType AII lantibiotic bovicin HJ50 with a rare disulfide bond: structure, structure-activity relationships and mode of actionStructural basis for trypanosomal haem acquisition and susceptibility to the host innate immune systemThe ring residue proline 8 is crucial for the thermal stability of the lasso peptide caulosegnin IIA conserved protein with AN1 zinc finger and ubiquitin-like domains modulates Cdc48 (p97) function in the ubiquitin-proteasome pathwayStrategies used for genetically modifying bacterial genome: site-directed mutagenesis, gene inactivation, and gene over-expressionPen-2 is incorporated into the gamma-secretase complex through binding to transmembrane domain 4 of presenilin 1Role of PelF in pel polysaccharide biosynthesis in Pseudomonas aeruginosaSerine protease MP2 activates prophenoloxidase in the melanization immune response of Drosophila melanogasterOne-Tube-Only Standardized Site-Directed Mutagenesis: An Alternative Approach to Generate Amino Acid Substitution CollectionsPerturbation in the conserved methyltransferase-polymerase interface of flavivirus NS5 differentially affects polymerase initiation and elongationFRET-based identification of mRNAs undergoing translationCofactor requirement of HpyAV restriction endonucleaseA Wolbachia deubiquitylating enzyme induces cytoplasmic incompatibilityDecreasing Transmembrane Segment Length Greatly Decreases Perfringolysin O Pore Size.Comparisons with amyloid-β reveal an aspartate residue that stabilizes fibrils of the aortic amyloid peptide medin.Structural insights unravel the zymogenic mechanism of the virulence factor gingipain K from Porphyromonas gingivalis, a causative agent of gum disease from the human oral microbiome.HIV virions as nanoscopic test tubes for probing oligomerization of the integrase enzyme.An artificial processivity clamp made with streptavidin facilitates oriented attachment of polymerase-DNA complexes to surfaces.Many families of C. elegans microRNAs are not essential for development or viability.Steering directed protein evolution: strategies to manage combinatorial complexity of mutant libraries.Metal A and metal B sites of nuclear RNA polymerases Pol IV and Pol V are required for siRNA-dependent DNA methylation and gene silencing.Trypanosoma brucei PUF9 regulates mRNAs for proteins involved in replicative processes over the cell cycle.Modes of calreticulin recruitment to the major histocompatibility complex class I assembly pathwayThe unique expression and function of miR-424 in human placental trophoblasts.Mechanisms of HDA6-mediated rRNA gene silencing: suppression of intergenic Pol II transcription and differential effects on maintenance versus siRNA-directed cytosine methylation.The ability of an attaching and effacing pathogen to trigger localized actin assembly contributes to virulence by promoting mucosal attachment.Comparison of the structure and function of phospholamban and the arginine-14 deficient mutant associated with dilated cardiomyopathy.Autographa californica multiple nucleopolyhedrovirus DNA polymerase C terminus is required for nuclear localization and viral DNA replicationThe C. elegans microRNA mir-71 acts in neurons to promote germline-mediated longevity through regulation of DAF-16/FOXOIdentification of the first inhibitor of the GBP1:PIM1 interaction. Implications for the development of a new class of anticancer agents against paclitaxel resistant cancer cells.An asymmetric PCR-based, reliable and rapid single-tube native DNA engineering strategy.Labeled EF-Tus for rapid kinetic studies of pretranslocation complex formationSpyAvidin hubs enable precise and ultrastable orthogonal nanoassembly.The polypeptide binding conformation of calreticulin facilitates its cell-surface expression under conditions of endoplasmic reticulum stress.Cell cycle sensing of oxidative stress in Saccharomyces cerevisiae by oxidation of a specific cysteine residue in the transcription factor Swi6p
P2860
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P2860
Site-directed, Ligase-Independent Mutagenesis (SLIM): a single-tube methodology approaching 100% efficiency in 4 h.
description
2004 nî lūn-bûn
@nan
2004 թուականի Դեկտեմբերին հրատարակուած գիտական յօդուած
@hyw
2004 թվականի դեկտեմբերին հրատարակված գիտական հոդված
@hy
2004年の論文
@ja
2004年論文
@yue
2004年論文
@zh-hant
2004年論文
@zh-hk
2004年論文
@zh-mo
2004年論文
@zh-tw
2004年论文
@wuu
name
Site-directed, Ligase-Independ ...... aching 100% efficiency in 4 h.
@ast
Site-directed, Ligase-Independ ...... aching 100% efficiency in 4 h.
@en
type
label
Site-directed, Ligase-Independ ...... aching 100% efficiency in 4 h.
@ast
Site-directed, Ligase-Independ ...... aching 100% efficiency in 4 h.
@en
prefLabel
Site-directed, Ligase-Independ ...... aching 100% efficiency in 4 h.
@ast
Site-directed, Ligase-Independ ...... aching 100% efficiency in 4 h.
@en
P2093
P2860
P356
P1476
Site-directed, Ligase-Independ ...... aching 100% efficiency in 4 h.
@en
P2093
Daniel Tillett
Joyce Chiu
Paul E March
P2860
P356
10.1093/NAR/GNH172
P407
P577
2004-12-07T00:00:00Z