Rapid insertional mutagenesis of DNA by polymerase chain reaction (PCR).
about
Functional role of the putative iron ligands in the ferroxidase activity of recombinant human hephaestinCyclic AMP (cAMP) and cAMP receptor protein influence both synthesis and uptake of extracellular autoinducer 2 in Escherichia coliRiboswitch-dependent gene regulation and its evolution in the plant kingdomA new approach to 'megaprimer' polymerase chain reaction mutagenesis without an intermediate gel purification stepDescription of a PCR-based technique for DNA splicing and mutagenesis by producing 5' overhangs with run through stop DNA synthesis utilizing Ara-C.A novel PCR strategy for high-efficiency, automated site-directed mutagenesis.Structure of the Toxoplasma gondii ROP18 Kinase Domain Reveals a Second Ligand Binding Pocket Required for Acute VirulenceStructure analysis of geranyl pyrophosphate methyltransferase and the proposed reaction mechanism of SAM-dependent C-methylationDistinct gene expression patterns in skeletal and cardiac muscle are dependent on common regulatory sequences in the MLC1/3 locusActivation of Go-coupled dopamine D2 receptors inhibits ERK1/ERK2 in pituitary cells. A key step in the transcriptional suppression of the prolactin geneHistone H2A.Z acid patch residues required for deposition and functionA new family of phosphotransferases with a P-loop motif.Assembly of designed oligonucleotides as an efficient method for gene recombination: a new tool in directed evolution.Site-directed, Ligase-Independent Mutagenesis (SLIM): a single-tube methodology approaching 100% efficiency in 4 h.Drosophila melanogaster positive transcriptional elongation factors regulate metabolic and sex-biased expression in adults.Use of a transient assay for studying the genetic determinants of Fv1 restriction.Rab7 is a critical mediator in vesicular transport of tyrosinase-related protein 1 in melanocytes.Functional comparison of the basic domains of the Tat proteins of human immunodeficiency virus types 1 and 2 in trans activation.Enhancement of telomere-plasmid segregation by the X-telomere associated sequence in Saccharomyces cerevisiae involves SIR2, SIR3, SIR4 and ABF1.DOF-binding sites additively contribute to guard cell-specificity of AtMYB60 promoter.Dual role of respiratory syncytial virus glycoprotein fragment as a mucosal immunogen and chemotactic adjuvantPromoter strength driving TetR determines the regulatory properties of Tet-controlled expression systemsStructure of apolipoprotein A-I N terminus on nascent high density lipoproteins.Duplication and diversification of the spermidine/spermine N1-acetyltransferase 1 genes in zebrafishCyclic nucleotide gated channels 7 and 8 are essential for male reproductive fertilityH+ -pumping rhodopsin from the marine alga Acetabularia.Systematic analysis of the amino acid residues of human papillomavirus type 16 E7 conserved region 3 involved in dimerization and transformation.Library construction, selection and modification strategies to generate therapeutic peptide-based modulators of protein-protein interactions.Functional analysis of the carboxy-terminal region of Bacillus subtilis TnrA, a MerR family protein.Construction of recombinant DNA molecules by the use of a single stranded DNA generated by the polymerase chain reaction: its application to chimeric hepatitis A virus/poliovirus subgenomic cDNA.The "beta-clasp" model of apolipoprotein A-I--a lipid-free solution structure determined by electron paramagnetic resonance spectroscopy.Monoclonal Antibody against G Glycoprotein Increases Respiratory Syncytial Virus Clearance In Vivo and Prevents Vaccine-Enhanced DiseasesCharge scan reveals an extended region at the intracellular end of the GABA receptor pore that can influence ion selectivity.Identification of Variant-Specific Functions of PIK3CA by Rapid Phenotyping of Rare MutationsFunctional annotation of rare gene aberration drivers of pancreatic cancerRobust one-tube Ω-PCR strategy accelerates precise sequence modification of plasmids for functional genomics.Stoichiometric pore mutations of the GABAAR reveal a pattern of hydrogen bonding with picrotoxin.Sarm1-mediated axon degeneration requires both SAM and TIR interactionsA human interleukin 3 analog with increased biological and binding activities.Functional analysis of the cell division protein FtsW of Escherichia coli.
P2860
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P2860
Rapid insertional mutagenesis of DNA by polymerase chain reaction (PCR).
description
1989 nî lūn-bûn
@nan
1989年の論文
@ja
1989年論文
@yue
1989年論文
@zh-hant
1989年論文
@zh-hk
1989年論文
@zh-mo
1989年論文
@zh-tw
1989年论文
@wuu
1989年论文
@zh
1989年论文
@zh-cn
name
Rapid insertional mutagenesis of DNA by polymerase chain reaction (PCR).
@en
type
label
Rapid insertional mutagenesis of DNA by polymerase chain reaction (PCR).
@en
prefLabel
Rapid insertional mutagenesis of DNA by polymerase chain reaction (PCR).
@en
P2093
P356
P1476
Rapid insertional mutagenesis of DNA by polymerase chain reaction (PCR).
@en
P2093
P356
10.1093/NAR/17.13.5404
P577
1989-07-01T00:00:00Z