Mutagenesis of the yeast gene PRP8 reveals domains governing the specificity and fidelity of 3' splice site selection.
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Human homologs of yeast prp16 and prp17 reveal conservation of the mechanism for catalytic step II of pre-mRNA splicingEvidence that U5 snRNP recognizes the 3' splice site for catalytic step II in mammalsPrp8 protein: at the heart of the spliceosomeThe C-terminal region of hPrp8 interacts with the conserved GU dinucleotide at the 5' splice siteThe human Prp8 protein is a component of both U2- and U12-dependent spliceosomesAn upstream AG determines whether a downstream AG is selected during catalytic step II of splicingThe architecture of the spliceosomal U4/U6.U5 tri-snRNP.Crystal structure of the -finger domain of Prp8 reveals analogy to ribosomal proteinsStructure and function of an RNase H domain at the heart of the spliceosomeCrystal structure of Prp8 reveals active site cavity of the spliceosomeStructural basis for dual roles of Aar2p in U5 snRNP assemblyA conformational switch in PRP8 mediates metal ion coordination that promotes pre-mRNA exon ligationExtensive genetic interactions between PRP8 and PRP17/CDC40, two yeast genes involved in pre-mRNA splicing and cell cycle progression.Evidence for a role of Sky1p-mediated phosphorylation in 3' splice site recognition involving both Prp8 and Prp17/Slu4.Synthetic lethality of yeast slt mutations with U2 small nuclear RNA mutations suggests functional interactions between U2 and U5 snRNPs that are important for both steps of pre-mRNA splicing.Comprehensive in vivo RNA-binding site analyses reveal a role of Prp8 in spliceosomal assembly.Spatial organization of protein-RNA interactions in the branch site-3' splice site region during pre-mRNA splicing in yeast.The Saccharomyces cerevisiae gene CDC40/PRP17 controls cell cycle progression through splicing of the ANC1 geneGenetic analysis reveals a role for the C terminus of the Saccharomyces cerevisiae GTPase Snu114 during spliceosome activationFunctional interactions between Prp8, Prp18, Slu7, and U5 snRNA during the second step of pre-mRNA splicing.Prp8 retinitis pigmentosa mutants cause defects in the transition between the catalytic steps of splicing.CEF1/CDC5 alleles modulate transitions between catalytic conformations of the spliceosome.Interaction of the yeast DExH-box RNA helicase prp22p with the 3' splice site during the second step of nuclear pre-mRNA splicingA mutation in a splicing factor that causes retinitis pigmentosa has a transcriptome-wide effect on mRNA splicing.Conserved loop I of U5 small nuclear RNA is dispensable for both catalytic steps of pre-mRNA splicing in HeLa nuclear extracts.Distinct domains of splicing factor Prp8 mediate different aspects of spliceosome activationGenetic interactions between the 5' and 3' splice site consensus sequences and U6 snRNA during the second catalytic step of pre-mRNA splicing.Ubiquitin binding by a variant Jab1/MPN domain in the essential pre-mRNA splicing factor Prp8p.Suppressors of a cold-sensitive mutation in yeast U4 RNA define five domains in the splicing factor Prp8 that influence spliceosome activation.Evidence for a linear search in bimolecular 3' splice site AG selection.Deletion of MUD2, the yeast homolog of U2AF65, can bypass the requirement for sub2, an essential spliceosomal ATPase.Allele-specific genetic interactions between Prp8 and RNA active site residues suggest a function for Prp8 at the catalytic core of the spliceosome.Functional interactions of Prp8 with both splice sites at the spliceosomal catalytic center.U2 toggles iteratively between the stem IIa and stem IIc conformations to promote pre-mRNA splicingFunctional roles of protein splicing factors.Exon ligation is proofread by the DExD/H-box ATPase Prp22p.A mutant allele of essential, general translation initiation factor DED1 selectively inhibits translation of a viral mRNARegulation of ceramide biosynthesis by TOR complex 2.Structural studies of the spliceosome: zooming into the heart of the machine.Probing of the spliceosome with site-specifically derivatized 5' splice site RNA oligonucleotides
P2860
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P2860
Mutagenesis of the yeast gene PRP8 reveals domains governing the specificity and fidelity of 3' splice site selection.
description
1996 nî lūn-bûn
@nan
1996 թուականի Յունիսին հրատարակուած գիտական յօդուած
@hyw
1996 թվականի հունիսին հրատարակված գիտական հոդված
@hy
1996年の論文
@ja
1996年論文
@yue
1996年論文
@zh-hant
1996年論文
@zh-hk
1996年論文
@zh-mo
1996年論文
@zh-tw
1996年论文
@wuu
name
Mutagenesis of the yeast gene ...... y of 3' splice site selection.
@ast
Mutagenesis of the yeast gene ...... y of 3' splice site selection.
@en
type
label
Mutagenesis of the yeast gene ...... y of 3' splice site selection.
@ast
Mutagenesis of the yeast gene ...... y of 3' splice site selection.
@en
prefLabel
Mutagenesis of the yeast gene ...... y of 3' splice site selection.
@ast
Mutagenesis of the yeast gene ...... y of 3' splice site selection.
@en
P2860
P1433
P1476
Mutagenesis of the yeast gene ...... y of 3' splice site selection.
@en
P2093
P2860
P304
P407
P577
1996-06-01T00:00:00Z