Segment-directed mutagenesis: construction in vitro of point mutations limited to a small predetermined region of a circular DNA molecule
about
DNA display I. Sequence-encoded routing of DNA populationsA novel method for site-directed mutagenesis: its application to an eukaryotic tRNAPro gene promoterThiol-beta-lactamase: replacement of the active-site serine of RTEM beta-lactamase by a cysteine residue.Effect of premature termination of translation on mRNA stability depends on the site of ribosome release.Simultaneous synthesis of human-, mouse- and chimeric epidermal growth factor genes via 'hybrid gene synthesis' approachEffects of all single base substitutions in the loop of boxB on antitermination of transcription by bacteriophage lambda's N protein.Adenovirus mutants with DNA sequence perturbations in the intragenic promoter of VAI RNA gene allow the enhanced transcription of VAII RNA gene in HeLa cells.Construction and expression in vivo of an internally deleted mouse alpha-fetoprotein gene: presence of a transcribed Alu-like repeat within the first intervening sequenceDeletion loop mutagenesis: a novel method for the construction of point mutations using deletion mutantsSegment-specific mutagenesis: extensive mutagenesis of a lac promoter/operator element.Gap misrepair mutagenesis: efficient site-directed induction of transition, transversion, and frameshift mutations in vitro.Oligonucleotide-directed mutagenesis as a general and powerful method for studies of protein function.Identification of wild-type or mutant alleles of bacterial genes cloned on a bacteriophage lambda vector: isolation of uvrC(am) and other mutants.A DNA fragment with an alpha-phosphorothioate nucleotide at one end is asymmetrically blocked from digestion by exonuclease III and can be replicated in vivo.Two alanine racemase genes in Salmonella typhimurium that differ in structure and functionDNA probes: applications of the principles of nucleic acid hybridization.Structural requirements of adenovirus VAI RNA for its translation enhancement function.Organization of the noncontiguous promoter components of adenovirus VAI RNA gene is strikingly similar to that of eucaryotic tRNA genes.Incomplete coverage of candidate genes: a poorly considered bias.Two-codon insertion mutagenesis of plasmid genes by using single-stranded hexameric oligonucleotidesBeta-lactamases: molecular studies.Characterization by deletion and localized mutagenesis in vitro of the promoter region of the Escherichia coli ompC gene and importance of the upstream DNA domain in positive regulation by the OmpR protein.Targeted mutagenesis in vitro: lac repressor mutations generated using AMV reverse transcriptase and dBrUTPA simple and efficient method for chemical mutagenesis of DNA.A rapid and efficient method for region- and strand-specific mutagenesis of cloned DNAThe use of oligonucleotide directed cleavage of DNA and homologous recombination in the production of large recombinant adenoviral vectors.
P2860
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P2860
Segment-directed mutagenesis: construction in vitro of point mutations limited to a small predetermined region of a circular DNA molecule
description
1980 nî lūn-bûn
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1980年学术文章
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name
Segment-directed mutagenesis: ...... ion of a circular DNA molecule
@ast
Segment-directed mutagenesis: ...... ion of a circular DNA molecule
@en
type
label
Segment-directed mutagenesis: ...... ion of a circular DNA molecule
@ast
Segment-directed mutagenesis: ...... ion of a circular DNA molecule
@en
prefLabel
Segment-directed mutagenesis: ...... ion of a circular DNA molecule
@ast
Segment-directed mutagenesis: ...... ion of a circular DNA molecule
@en
P2093
P2860
P356
P1476
Segment-directed mutagenesis: ...... ion of a circular DNA molecule
@en
P2093
D Koshland
G M Weinstock
P2860
P304
P356
10.1073/PNAS.77.9.5375
P407
P577
1980-09-01T00:00:00Z