Invariant U2 RNA sequences bordering the branchpoint recognition region are essential for interaction with yeast SF3a and SF3b subunits.
about
Characterization of a protein complex containing spliceosomal proteins SAPs 49, 130, 145, and 155CUS2, a yeast homolog of human Tat-SF1, rescues function of misfolded U2 through an unusual RNA recognition motifPrp5 bridges U1 and U2 snRNPs and enables stable U2 snRNP association with intron RNADomains in human splicing factors SF3a60 and SF3a66 required for binding to SF3a120, assembly of the 17S U2 snRNP, and prespliceosome formationCombined biochemical and electron microscopic analyses reveal the architecture of the mammalian U2 snRNPModulating splicing with small molecular inhibitors of the spliceosomeSolution structure of the U2 snRNP protein Rds3p reveals a knotted zinc-finger motifStructure and assembly of the SF3a splicing factor complex of U2 snRNPSynthetic lethality of yeast slt mutations with U2 small nuclear RNA mutations suggests functional interactions between U2 and U5 snRNPs that are important for both steps of pre-mRNA splicing.Conservation of structure and subunit interactions in yeast homologues of splicing factor 3b (SF3b) subunits.Comprehensive in vivo RNA-binding site analyses reveal a role of Prp8 in spliceosomal assembly.The Saccharomyces cerevisiae Prp5 protein has RNA-dependent ATPase activity with specificity for U2 small nuclear RNA.Pseudouridine mapping in the Saccharomyces cerevisiae spliceosomal U small nuclear RNAs (snRNAs) reveals that pseudouridine synthase pus1p exhibits a dual substrate specificity for U2 snRNA and tRNA.Rearrangement of competing U2 RNA helices within the spliceosome promotes multiple steps in splicingA novel yeast U2 snRNP protein, Snu17p, is required for the first catalytic step of splicing and for progression of spliceosome assembly.Structural and mechanistic insights into human splicing factor SF3b complex derived using an integrated approach guided by the cryo-EM density mapsA ribozyme selected from variants of U6 snRNA promotes 2',5'-branch formationThe ATP requirement for U2 snRNP addition is linked to the pre-mRNA region 5' to the branch site.RNA structure analysis of human spliceosomes reveals a compact 3D arrangement of snRNAs at the catalytic core.ATP requirement for Prp5p function is determined by Cus2p and the structure of U2 small nuclear RNA.Invariant U2 snRNA nucleotides form a stem loop to recognize the intron early in splicing.Breaking up the C complex spliceosome shows stable association of proteins with the lariat intron intermediateSite-specific deoxynucleotide substitutions in yeast U6 snRNA block splicing of pre-mRNA in vitro.Depletion of yeast RNase III blocks correct U2 3' end formation and results in polyadenylated but functional U2 snRNA.Multiple developmental requirements of noisette, the Drosophila homolog of the U2 snRNP-associated polypeptide SP3a60.Rearrangements within human spliceosomes captured after exon ligation.A limited number of pseudouridine residues in the human atac spliceosomal UsnRNAs as compared to human major spliceosomal UsnRNAsA tertiary interaction detected in a human U2-U6 snRNA complex assembled in vitro resembles a genetically proven interaction in yeast.The conserved central domain of yeast U6 snRNA: importance of U2-U6 helix Ia in spliceosome assembly.Genetic interaction mapping reveals a role for the SWI/SNF nucleosome remodeler in spliceosome activation in fission yeastU2 toggles iteratively between the stem IIa and stem IIc conformations to promote pre-mRNA splicingRNA-mediated interaction of Cajal bodies and U2 snRNA genes.Conformational heterogeneity of the protein-free human spliceosomal U2-U6 snRNA complexInhibition of pre-mRNA splicing by synthetic branched nucleic acids.Endogenous U2·U5·U6 snRNA complexes in S. pombe are intron lariat spliceosomes.The mechanism of the second step of pre-mRNA splicing.Probing interactions between the U2 small nuclear ribonucleoprotein and the DEAD-box protein, Prp5.Sequences upstream of the branch site are required to form helix II between U2 and U6 snRNA in a trans-splicing reaction.Evolution of small nuclear RNAs in S. cerevisiae, C. albicans, and other hemiascomycetous yeasts.Evidence that U2/U6 helix I promotes both catalytic steps of pre-mRNA splicing and rearranges in between these steps.
P2860
Q22010545-ECA20F8D-66D0-4AC2-8AC7-B26311DA335AQ24522636-5DB83D14-9C6E-42B0-84A3-6111317D4829Q24535910-6EF4CA60-0286-42C7-9CA2-B8780AD5C9BDQ24548345-9D3030F6-9DEC-419A-940D-E3B48AF76699Q24682070-6F3A583A-2150-48A2-B2D9-665151CD3B5EQ26738664-A2DB5EC0-8169-43F1-B94A-BE69E41BB86CQ27651175-521CAFD3-7635-4FF0-A441-EE2A471D21EEQ27677135-0CBD3AB2-E337-4BB9-98E0-6EE11EAF824AQ27933470-5206DFDC-9EAE-4AB0-9B43-0783588C231AQ27934190-60276D24-4369-420E-9C4D-32040E2EB800Q27934194-851F8732-F9FF-4B61-95F7-F47309346340Q27937327-DB2974FA-02C4-413C-BB21-DF78330D8577Q27937578-A15E08CA-6B08-4FAE-988F-6BD97291C2C3Q27939473-0B59717A-3EBE-4D2A-94C5-8A87A4E016A1Q27939744-4DBD6E63-C952-4DEF-8AD8-BBFCE56C34B8Q28315106-AE8BDF61-9AF3-46E5-9133-17E6DD0F4548Q28352546-6600F273-D4D1-4C86-B3C6-2C71FB10768FQ28359568-8E0CFE4D-1060-4E88-B97E-E0A211E9CA55Q30556912-88B6E8F8-61FD-4B06-BDAC-D7C1AC720433Q33713419-83905155-3C05-409A-B85B-ABFE150E8992Q33861218-BD807D2E-BDC5-4003-AEDA-82DDCC292C71Q33883523-87E97622-F7A9-4FB3-9CD6-0B88274951CBQ33886470-C38F1657-CE94-4CEB-89A6-FBA99D581428Q33889066-EC483020-D06A-411A-9051-62379A0B01EBQ33994028-1B0A8788-721E-4FF7-8753-2369382F6CA0Q34324094-6C437D72-9311-4997-BC66-608B8EED7306Q34362243-B9EF3112-A5B8-4B0C-959C-A216704E53D9Q34362402-0AE812A7-0309-4848-9DE7-4313001E332CQ34364667-E2572664-59E7-400D-AD07-A35208E4D077Q35235099-BB2F375B-D876-40BB-9017-094952E9804BQ35720890-2793E24E-02E7-4BA2-8D62-95495C906B72Q36377543-9B9A81AC-05A6-46EB-B21B-42AC609CFA9AQ36915058-4BF773F4-29C1-46CF-8880-C429CD97CF6DQ37369081-25A8EECA-C7CE-40D2-8BBF-F539EA357997Q37581520-57669DE5-FFFA-447C-96B8-2EFF87588292Q37947529-5B658C1F-653B-47DC-BA55-43B84597B036Q38290577-E55B701F-A767-4297-BBB2-1258BB64B320Q38678938-141CCC2C-2184-4EF2-AFD2-E9EE5812A580Q40189605-C696C6FE-C047-47B4-BBE3-541128642B2EQ41764126-F799ED0C-D089-4E1C-8757-6448473A93A9
P2860
Invariant U2 RNA sequences bordering the branchpoint recognition region are essential for interaction with yeast SF3a and SF3b subunits.
description
1996 nî lūn-bûn
@nan
1996年の論文
@ja
1996年論文
@yue
1996年論文
@zh-hant
1996年論文
@zh-hk
1996年論文
@zh-mo
1996年論文
@zh-tw
1996年论文
@wuu
1996年论文
@zh
1996年论文
@zh-cn
name
Invariant U2 RNA sequences bor ...... yeast SF3a and SF3b subunits.
@ast
Invariant U2 RNA sequences bor ...... yeast SF3a and SF3b subunits.
@en
type
label
Invariant U2 RNA sequences bor ...... yeast SF3a and SF3b subunits.
@ast
Invariant U2 RNA sequences bor ...... yeast SF3a and SF3b subunits.
@en
prefLabel
Invariant U2 RNA sequences bor ...... yeast SF3a and SF3b subunits.
@ast
Invariant U2 RNA sequences bor ...... yeast SF3a and SF3b subunits.
@en
P2860
P356
P1476
Invariant U2 RNA sequences bor ...... yeast SF3a and SF3b subunits.
@en
P2860
P304
P356
10.1128/MCB.16.3.818
P407
P577
1996-03-01T00:00:00Z