Random circular permutation of genes and expressed polypeptide chains: application of the method to the catalytic chains of aspartate transcarbamoylase.
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Circular permutation of 5-aminolevulinate synthase. Mapping the polypeptide chain to its functionCircularly permuted variants of the green fluorescent proteinRandom circular permutation leading to chain disruption within and near alpha helices in the catalytic chains of aspartate transcarbamoylase: effects on assembly, stability, and functionCircular permutation and receptor insertion within green fluorescent proteinsAssessment of the allosteric mechanism of aspartate transcarbamoylase based on the crystalline structure of the unregulated catalytic subunitBinding of bisubstrate analog promotes large structural changes in the unregulated catalytic trimer of aspartate transcarbamoylase: Implications for allosteric regulationProtein design by fusion: implications for protein structure prediction and evolutionStructure of pvu II DNA-(cytosine N4) methyltransferase, an example of domain permutation and protein fold assignmentCrystal structures and properties of de novo circularly permuted 1,3-1,4-beta-glucanasesStructure and function of the Bacillus hybrid enzyme GluXyn-1: native-like jellyroll fold preserved after insertion of autonomous globular domainTesting the role of chain connectivity on the stability and structure of dihydrofolate reductase from E. coli: fragment complementation and circular permutation reveal stable, alternatively folded formsTolerance of a Knotted Near-Infrared Fluorescent Protein to Random Circular PermutationComplementation and reconstitution of fluorescence from circularly permuted and truncated green fluorescent proteinProtein reconstitution and three-dimensional domain swapping: benefits and constraints of covalency.Construction of Allosteric Protein Switches by Alternate Frame Folding and Intermolecular Fragment Exchange.Combinatorial protein engineering by incremental truncationLibraries of hybrid proteins from distantly related sequences.Directed evolution of a biterminal bacterial display scaffold enhances the display of diverse peptidesRandom dissection to select for protein split sites and its application in protein fragment complementationConsolidating critical binding determinants by noncyclic rearrangement of protein secondary structure.Protein switches identified from diverse insertion libraries created using S1 nuclease digestion of supercoiled-form plasmid DNAProbing the functional mechanism of Escherichia coli GroEL using circular permutation.Protein and RNA engineering to customize microbial molecular reporting.A transposase strategy for creating libraries of circularly permuted proteins.Circular permutation in the Ω-loop of TEM-1 β-lactamase results in improved activity and altered substrate specificityDiversity in genetic in vivo methods for protein-protein interaction studies: from the yeast two-hybrid system to the mammalian split-luciferase system.An automated flow for directed evolution based on detection of promiscuous scaffolds using spatial and electrostatic properties of catalytic residues.The Structure of a Thermophilic Kinase Shapes Fitness upon Random Circular PermutationA xylose-stimulated xylanase-xylose binding protein chimera created by random nonhomologous recombination.PERMutation Using Transposase Engineering (PERMUTE): A Simple Approach for Constructing Circularly Permuted Protein Libraries.Engineering allosteric protein switches by domain insertion.Thermodynamic and structural consequences of flexible loop deletion by circular permutation in the streptavidin-biotin systemCommunication between RNA folding domains revealed by folding of circularly permuted ribozymes.In vivo assembly of aspartate transcarbamoylase from fragmented and circularly permuted catalytic polypeptide chains.Charge neutralization in the active site of the catalytic trimer of aspartate transcarbamoylase promotes diverse structural changes.Facilitating circular permutation using Restriction Free (RF) cloning.Engineering of Yarrowia lipolytica lipase Lip8p by circular permutation to alter substrate and temperature characteristics.Evolution of new protein topologies through multistep gene rearrangements
P2860
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P2860
Random circular permutation of genes and expressed polypeptide chains: application of the method to the catalytic chains of aspartate transcarbamoylase.
description
article científic
@ca
article scientifique
@fr
articolo scientifico
@it
artigo científico
@pt
bilimsel makale
@tr
scientific article published on October 1996
@en
vedecký článok
@sk
vetenskaplig artikel
@sv
videnskabelig artikel
@da
vědecký článek
@cs
name
Random circular permutation of ...... f aspartate transcarbamoylase.
@en
Random circular permutation of ...... f aspartate transcarbamoylase.
@nl
type
label
Random circular permutation of ...... f aspartate transcarbamoylase.
@en
Random circular permutation of ...... f aspartate transcarbamoylase.
@nl
prefLabel
Random circular permutation of ...... f aspartate transcarbamoylase.
@en
Random circular permutation of ...... f aspartate transcarbamoylase.
@nl
P2860
P356
P1476
Random circular permutation of ...... f aspartate transcarbamoylase.
@en
P2093
P2860
P304
11591-11596
P356
10.1073/PNAS.93.21.11591
P407
P577
1996-10-01T00:00:00Z