Expression and site-specific mutagenesis of the poliovirus 3C protease in Escherichia coli.
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Molecular and biotechnological aspects of microbial proteasesViral cysteine proteases are homologous to the trypsin-like family of serine proteases: structural and functional implicationsThe refined crystal structure of the 3C gene product from hepatitis A virus: specific proteinase activity and RNA recognitionEvidence that the N-terminal domain of nonstructural protein NS3 from yellow fever virus is a serine protease responsible for site-specific cleavages in the viral polyproteinCharacterization of Bafinivirus main protease autoprocessing activities.Cysteine proteases of positive strand RNA viruses and chymotrypsin-like serine proteases. A distinct protein superfamily with a common structural fold.The picornaviral 3C proteinases: cysteine nucleophiles in serine proteinase folds.A norovirus protease structure provides insights into active and substrate binding site integrityIdentification of active-site amino acid residues in the Chiba virus 3C-like proteaseExpression of enzymatically active poliovirus RNA-dependent RNA polymerase in Escherichia coli.Two novel heat shock genes encoding proteins produced in response to heterologous protein expression in Escherichia coliPoliovirus thiol proteinase 3C can utilize a serine nucleophile within the putative catalytic triad.Identification of active-site residues in protease 3C of hepatitis A virus by site-directed mutagenesis.Direct evidence of a role for amino acid 101 of VP-1 in central nervous system disease in Theiler's murine encephalomyelitis virus infection.Proteolytic activity of hepatitis A virus 3C proteintrans rescue of a mutant poliovirus RNA polymerase functioncis- and trans-cleavage activities of poliovirus 2A protease expressed in Escherichia coli.Chimeric picornavirus polyproteins demonstrate a common 3C proteinase substrate specificityEffects of mutations in poliovirus 3Dpol on RNA polymerase activity and on polyprotein cleavage.Poliovirus proteinase 3C: large-scale expression, purification, and specific cleavage activity on natural and synthetic substrates in vitro.Proteolytic processing of foot-and-mouth disease virus polyproteins expressed in a cell-free system from clone-derived transcripts.Expression of virus-encoded proteinases: functional and structural similarities with cellular enzymesRegulation of poliovirus 3C protease by the 2C polypeptideA rapid method for determination of endoproteinase substrate specificity: specificity of the 3C proteinase from hepatitis A virus.Cleavage of small peptides in vitro by human rhinovirus 14 3C protease expressed in Escherichia coli.Viral proteases: an emerging therapeutic target.Novel bacteriological assay for detection of potential antiviral agents.Substitution of cysteine 192 in a highly conserved Streptococcus pyogenes extracellular cysteine protease (interleukin 1beta convertase) alters proteolytic activity and ablates zymogen processing.Identification and characterization of a 3C-like protease from rabbit hemorrhagic disease virus, a calicivirus.Foot-and-mouth disease virus protease 3C induces specific proteolytic cleavage of host cell histone H3.Expression of tetanus toxin fragment C in E. coli: high level expression by removing rare codons.Separation of native and truncated forms of poliovirus protease 3C produced in Escherichia coli.Comparative analysis of viral cysteine protease structural models.Proteases and protease inhibitors in infectious diseases.Recombinant poliovirus 3C protease. The enzyme application to protein specific fragmentation.
P2860
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P2860
Expression and site-specific mutagenesis of the poliovirus 3C protease in Escherichia coli.
description
article científic
@ca
article scientifique
@fr
articolo scientifico
@it
artigo científico
@pt
bilimsel makale
@tr
scientific article published on August 1986
@en
vedecký článok
@sk
vetenskaplig artikel
@sv
videnskabelig artikel
@da
vědecký článek
@cs
name
Expression and site-specific m ...... protease in Escherichia coli.
@en
Expression and site-specific m ...... protease in Escherichia coli.
@nl
type
label
Expression and site-specific m ...... protease in Escherichia coli.
@en
Expression and site-specific m ...... protease in Escherichia coli.
@nl
prefLabel
Expression and site-specific m ...... protease in Escherichia coli.
@en
Expression and site-specific m ...... protease in Escherichia coli.
@nl
P2093
P2860
P356
P1476
Expression and site-specific m ...... protease in Escherichia coli.
@en
P2093
P2860
P304
P356
10.1073/PNAS.83.15.5392
P407
P577
1986-08-01T00:00:00Z