Double targeted gene replacement for creating null mutants. - Wikidata - awgldk - TriplyDB

Double targeted gene replacement for creating null mutants.

Double targeted gene replacement for creating null mutants.

http://www.wikidata.org/entity/Q37578939

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Comparative genomic analysis of three Leishmania species that cause diverse human disease Functional identification of a Leishmania gene related to the peroxin 2 gene reveals common ancestry of glycosomes and peroxisomes Development and validation of a cytochrome c-coupled assay for pteridine reductase 1 and dihydrofolate reductase Crystal structure of theLeishmania majorMIX protein: A scaffold protein that mediates protein-protein interactions Disruption of mannose activation in Leishmania mexicana: GDP-mannose pyrophosphorylase is required for virulence, but not for viability.Golgi-located NTPDase1 of Leishmania major is required for lipophosphoglycan elongation and normal lesion development whereas secreted NTPDase2 is dispensable for virulence Proteophosphoglycans of Leishmania mexicana. Molecular cloning and characterization of the Leishmania mexicana ppg2 gene encoding the proteophosphoglycans aPPG and pPPG2 that are secreted by amastigotes and promastigotes.A physical map of the Leishmania major Friedlin genome Gene disruption of the DNA topoisomerase IB small subunit induces a non-viable phenotype in the hemoflagellate Leishmania major Evaluation of high efficiency gene knockout strategies for Trypanosoma cruzi Glyoxalase I gene deletion mutants of Leishmania donovani exhibit reduced methylglyoxal detoxification Ascorbate peroxidase from Leishmania major controls the virulence of infective stage of promastigotes by regulating oxidative stress.Identifying vaccine targets for anti-leishmanial vaccine development Development of a safe live Leishmania vaccine line by gene replacement.Effect of large targeted deletions on the mitotic stability of an extra chromosome mediating drug resistance in Leishmania.Isogenic strain construction and gene mapping in Candida albicans.Expansion of the target of rapamycin (TOR) kinase family and function in Leishmania shows that TOR3 is required for acidocalcisome biogenesis and animal infectivity.Expression of green fluorescent protein as a marker for effects of antileishmanial compounds in vitro Glycosylation defects and virulence phenotypes of Leishmania mexicana phosphomannomutase and dolicholphosphate-mannose synthase gene deletion mutants.Characterization of a Leishmania stage-specific mitochondrial membrane protein that enhances the activity of cytochrome c oxidase and its role in virulence Knockout of the dhfr-ts gene in Trypanosoma cruzi generates attenuated parasites able to confer protection against a virulent challenge.The role of the mitochondrial glycine cleavage complex in the metabolism and virulence of the protozoan parasite Leishmania major Target assessment for antiparasitic drug discovery.Phenylalanine hydroxylase (PAH) from the lower eukaryote Leishmania major.The roles of pteridine reductase 1 and dihydrofolate reductase-thymidylate synthase in pteridine metabolism in the protozoan parasite Leishmania major.A rapid, efficient and economical method for generating leishmanial gene targeting constructs.Functional analysis of Leishmania cyclopropane fatty acid synthetase Leishmania major methionine sulfoxide reductase A is required for resistance to oxidative stress and efficient replication in macrophages.Disruption of the Crithidia fasciculata RNH1 gene results in the loss of two active forms of ribonuclease H.Generating knock-in parasites: integration of an ornithine decarboxylase transgene into its chromosomal locus in Leishmania donovani Enhanced activity of antisense phosphorothioate oligos against leishmania amastigotes: augmented uptake of oligo, ribonuclease H activation, and efficient target intervention under altered growth conditions.Genetic characterization of glucose transporter function in Leishmania mexicana Increased efficacy of antileishmanial antisense phosphorothioate oligonucleotides in Leishmania amazonensis overexpressing ribonuclease H Lipophosphoglycan is a virulence factor distinct from related glycoconjugates in the protozoan parasite Leishmania major.Functional characterization of nucleoside transporter gene replacements in Leishmania donovani Target validation: linking target and chemical properties to desired product profile Sequence elements in both the intergenic space and the 3' untranslated region of the Crithidia fasciculata KAP3 gene are required for cell cycle regulation of KAP3 mRNA.The genetic toolbox for Leishmania parasites High-efficiency homologous recombination in the oil-producing alga Nannochloropsis sp.Centrin gene disruption impairs stage-specific basal body duplication and cell cycle progression in Leishmania.

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Double targeted gene replacement for creating null mutants.

http://www.wikidata.org/entity/Q37578939

description

article científic

@ca

article scientifique

@fr

articolo scientifico

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artigo científico

@pt

bilimsel makale

@tr

scientific article published on August 1991

@en

vedecký článok

@sk

vetenskaplig artikel

@sv

videnskabelig artikel

@da

vědecký článek

@cs

name

Double targeted gene replacement for creating null mutants.

@en

Double targeted gene replacement for creating null mutants.

@nl

type

label

Double targeted gene replacement for creating null mutants.

@en

Double targeted gene replacement for creating null mutants.

@nl

prefLabel

Double targeted gene replacement for creating null mutants.

@en

Double targeted gene replacement for creating null mutants.

@nl

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PNAS.88.16.7170

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Proceedings of the National Academy of Sciences of the United States of America

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Double targeted gene replacement for creating null mutants.

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Beverley SM

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Coburn CM

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Cruz A

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P2860

A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity

Plasmid-encoded hygromycin B resistance: the sequence of hygromycin B phosphotransferase gene and its expression in Escherichia coli and Saccharomyces cerevisiae

Altering the genome by homologous recombination

Targeting of nonexpressed genes in embryonic stem cells via homologous recombination

Disruption of the proto-oncogene int-2 in mouse embryo-derived stem cells: a general strategy for targeting mutations to non-selectable genes

Site-directed mutagenesis by gene targeting in mouse embryo-derived stem cells

[12] One-step gene disruption in yeast

Development of a stable Leishmania expression vector and application to the study of parasite surface antigen genes.

High-frequency disruption of the N-myc gene in embryonic stem and pre-B cell lines by homologous recombination.

In vitro activity of sulfonamides and sulfones against Leishmania major promastigotes.

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7170-7174

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scholarly article

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10.1073/PNAS.88.16.7170

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1991-08-01T00:00:00Z

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1651496

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