Directed evolution of a para-nitrobenzyl esterase for aqueous-organic solvents.
about
Biological consequences of ancient gene acquisition and duplication in the large genome of Candidatus Solibacter usitatus Ellin6076RNA-peptide fusions for the in vitro selection of peptides and proteinsA structural view of evolutionary divergenceCrystal structure of hyperthermophilic esterase EstE1 and the relationship between its dimerization and thermostability propertiesDieselzymes: development of a stable and methanol tolerant lipase for biodiesel production by directed evolutionRedesigning the substrate specificity of an enzyme by cumulative effects of the mutations of non-active site residuesDNA shuffling of a family of genes from diverse species accelerates directed evolutionDirected evolution of ampicillin-resistant activity from a functionally unrelated DNA fragment: A laboratory model of molecular evolutionComputational method to reduce the search space for directed protein evolution.Creating multiple-crossover DNA libraries independent of sequence identity.Bacterial cell surface display of an enzyme library for selective screening of improved cellulase variantsGrowth factor engineering by degenerate homoduplex gene family recombination.Significantly enhanced stability of glucose dehydrogenase by directed evolution.High efficiency family shuffling based on multi-step PCR and in vivo DNA recombination in yeast: statistical and functional analysis of a combinatorial library between human cytochrome P450 1A1 and 1A2Directed evolution strategies for enantiocomplementary haloalkane dehalogenases: from chemical waste to enantiopure building blocks.Saturation mutagenesis of toluene ortho-monooxygenase of Burkholderia cepacia G4 for Enhanced 1-naphthol synthesis and chloroform degradationScreening, nucleotide sequence, and biochemical characterization of an esterase from Pseudomonas fluorescens with high activity towards lactonesStepwise in vitro affinity maturation of Vitaxin, an alphav beta3-specific humanized mAb.Random-priming in vitro recombination: an effective tool for directed evolution.Improving the carboligase activity of benzoylformate decarboxylase from Pseudomonas putida by a combination of directed evolution and site-directed mutagenesis.Structural and catalytic response to temperature and cosolvents of carboxylesterase EST1 from the extremely thermoacidophilic archaeon Sulfolobus solfataricus P1.Improved catalytic efficiency and active site modification of 1,4-beta-D-glucan glucohydrolase A from Thermotoga neapolitana by directed evolution.Thermostable mutants of the photoprotein aequorin obtained by in vitro evolution.Enhanced reactivity of Rhizopus oryzae lipase displayed on yeast cell surfaces in organic solvents: potential as a whole-cell biocatalyst in organic solventsScreening mutant libraries of fungal laccases in the presence of organic solvents.Laboratory-directed protein evolutionHeterologous overexpression of a mutant termite cellulase gene in Escherichia coli by DNA shuffling of four orthologous parental cDNAs.Diversification and specialization of HIV protease function during in vitro evolution.Directed evolution: an approach to engineer enzymes.Prospecting metagenomic enzyme subfamily genes for DNA family shuffling by a novel PCR-based approachDirected evolution of a recombinase for improved genomic integration at a native human sequence.Predicting evolution by in vitro evolution requires determining evolutionary pathways.Discovery of superior enzymes by directed molecular evolution.Predicting evolutionary potential: in vitro evolution accurately reproduces natural evolution of the tem beta-lactamase.Microbial carboxyl esterases: classification, properties and application in biocatalysis.Directed evolution of aminoglycoside phosphotransferase (3') type IIIa variants that inactivate amikacin but impose significant fitness costs.A panel of cytochrome P450 BM3 variants to produce drug metabolites and diversify lead compounds.Computational design and selections for an engineered, thermostable terpene synthase.Expanding the enzyme universe: accessing non-natural reactions by mechanism-guided directed evolution.A first continuous 4-aminoantipyrine (4-AAP)-based screening system for directed esterase evolution.
P2860
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P2860
Directed evolution of a para-nitrobenzyl esterase for aqueous-organic solvents.
description
1996 nî lūn-bûn
@nan
1996年の論文
@ja
1996年学术文章
@wuu
1996年学术文章
@zh-cn
1996年学术文章
@zh-hans
1996年学术文章
@zh-my
1996年学术文章
@zh-sg
1996年學術文章
@yue
1996年學術文章
@zh
1996年學術文章
@zh-hant
name
Directed evolution of a para-nitrobenzyl esterase for aqueous-organic solvents.
@en
type
label
Directed evolution of a para-nitrobenzyl esterase for aqueous-organic solvents.
@en
prefLabel
Directed evolution of a para-nitrobenzyl esterase for aqueous-organic solvents.
@en
P2860
P356
P1433
P1476
Directed evolution of a para-nitrobenzyl esterase for aqueous-organic solvents.
@en
P2093
P2860
P2888
P304
P356
10.1038/NBT0496-458
P577
1996-04-01T00:00:00Z