Using 2-aminopurine fluorescence to measure incorporation of incorrect nucleotides by wild type and mutant bacteriophage T4 DNA polymerases.
about
Characterization of a replicative DNA polymerase mutant with reduced fidelity and increased translesion synthesis capacityVariation in Mutation Rates Caused by RB69pol Fidelity Mutants Can Be Rationalized on the Basis of Their Kinetic Behavior and Crystal StructuresStructure of the 2-Aminopurine-Cytosine Base Pair Formed in the Polymerase Active Site of the RB69 Y567A-DNA PolymeraseUsing a Fluorescent Cytosine Analogue tC o To Probe the Effect of the Y567 to Ala Substitution on the Preinsertion Steps of dNMP Incorporation by RB69 DNA PolymeraseAlteration in the cavity size adjacent to the active site of RB69 DNA polymerase changes its conformational dynamicsSensitivity to phosphonoacetic acid: a new phenotype to probe DNA polymerase delta in Saccharomyces cerevisiae.Identification of a new motif in family B DNA polymerases by mutational analyses of the bacteriophage t4 DNA polymerase.Effect of A and B metal ion site occupancy on conformational changes in an RB69 DNA polymerase ternary complex.Investigating local conformations of double-stranded DNA by low-energy circular dichroism of pyrrolo-cytosine.Engineering processive DNA polymerases with maximum benefit at minimum costElucidation of kinetic mechanisms of human translesion DNA polymerase κ using tryptophan mutants.Low-energy circular dichroism of 2-aminopurine dinucleotide as a probe of local conformation of DNA and RNA.Reversal of a mutator activity by a nearby fidelity-neutral substitution in the RB69 DNA polymerase binding pocket.Single-molecule studies of DNA replisome function.Use of 2-aminopurine fluorescence to study the role of the beta hairpin in the proofreading pathway catalyzed by the phage T4 and RB69 DNA polymerasesInefficient proofreading and biased error rates during inaccurate DNA synthesis by a mutant derivative of Saccharomyces cerevisiae DNA polymerase delta.Regulation of B family DNA polymerase fidelity by a conserved active site residue: characterization of M644W, M644L and M644F mutants of yeast DNA polymerase epsilon.Single-molecule and ensemble fluorescence assays for a functionally important conformational change in T7 DNA polymerase.Fluorescence of 2-aminopurine reveals rapid conformational changes in the RB69 DNA polymerase-primer/template complexes upon binding and incorporation of matched deoxynucleoside triphosphates.Mutator alleles of yeast DNA polymerase zeta.Dividing the workload at a eukaryotic replication fork.Low-fidelity DNA synthesis by the L979F mutator derivative of Saccharomyces cerevisiae DNA polymerase zeta.Techniques used to study the DNA polymerase reaction pathway.Fluorescent DNA-based enzyme sensors.Site-specific variations in RNA folding thermodynamics visualized by 2-aminopurine fluorescence.Low energy CD of RNA hairpin unveils a loop conformation required for lambdaN antitermination activity.Luminescent Strategies for Label-free G-quadruplex-based Enzyme Activity Sensing.Differences in replication of a DNA template containing an ethyl phosphotriester by T4 DNA polymerase and Escherichia coli DNA polymerase I.Dynamics of nucleotide incorporation: snapshots revealed by 2-aminopurine fluorescence studies.DNA polymerase proofreading: active site switching catalyzed by the bacteriophage T4 DNA polymerase.Fluorescence-based assay to measure the real-time kinetics of nucleotide incorporation during transcription elongation.Recent progress in dissecting molecular recognition by DNA polymerases with non-native substrates.Exponential quadruplex priming amplification for DNA-based isothermal diagnostics.Isothermal amplification of DNA using quadruplex primers with fluorescent pteridine base analogue 3-methyl isoxanthopterin.
P2860
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P2860
Using 2-aminopurine fluorescence to measure incorporation of incorrect nucleotides by wild type and mutant bacteriophage T4 DNA polymerases.
description
2002 nî lūn-bûn
@nan
2002年の論文
@ja
2002年学术文章
@wuu
2002年学术文章
@zh-cn
2002年学术文章
@zh-hans
2002年学术文章
@zh-my
2002年学术文章
@zh-sg
2002年學術文章
@yue
2002年學術文章
@zh
2002年學術文章
@zh-hant
name
Using 2-aminopurine fluorescen ...... teriophage T4 DNA polymerases.
@en
type
label
Using 2-aminopurine fluorescen ...... teriophage T4 DNA polymerases.
@en
prefLabel
Using 2-aminopurine fluorescen ...... teriophage T4 DNA polymerases.
@en
P2093
P2860
P356
P1476
Using 2-aminopurine fluorescen ...... teriophage T4 DNA polymerases.
@en
P2093
Elizabeth Fidalgo da Silva
Linda J Reha-Krantz
Subhrangsu S Mandal
P2860
P304
40640-40649
P356
10.1074/JBC.M203315200
P407
P577
2002-08-19T00:00:00Z