Engineered alkane-hydroxylating cytochrome P450(BM3) exhibiting nativelike catalytic properties.
about
In the light of directed evolution: pathways of adaptive protein evolutionExploring protein fitness landscapes by directed evolutionHydrocarbon hydroxylation by cytochrome P450 enzymesDetection of substrate-dependent conformational changes in the P450 fold by nuclear magnetic resonance.Key Mutations Alter the Cytochrome P450 BM3 Conformational Landscape and Remove Inherent Substrate BiasEngineering cytochrome P450 biocatalysts for biotechnology, medicine and bioremediationEvolutionary history of a specialized p450 propane monooxygenaseOlefin Cyclopropanation via Carbene Transfer Catalyzed by Engineered Cytochrome P450 EnzymesInduction of multiple pleiotropic drug resistance genes in yeast engineered to produce an increased level of anti-malarial drug precursor, artemisinic acid.Consensus protein design without phylogenetic biasEnzyme Efficiency but Not Thermostability Drives Cefotaxime Resistance Evolution in TEM-1 β-Lactamase.Screening of a minimal enriched P450 BM3 mutant library for hydroxylation of cyclic and acyclic alkanes.Enhancing the efficiency and regioselectivity of P450 oxidation catalysts by unnatural amino acid mutagenesis.Engineering of LadA for enhanced hexadecane oxidation using random- and site-directed mutagenesis.Changing the regioselectivity of a P450 from C15 to C11 hydroxylation of progesterone.Directed evolution of RebH for site-selective halogenation of large biologically active molecules.Directed evolution of protein-based neurotransmitter sensors for MRIDesign of functional metalloproteins.Enzymatic functionalization of carbon-hydrogen bonds.Directed evolution of a magnetic resonance imaging contrast agent for noninvasive imaging of dopamine.Directed evolution of aminoglycoside phosphotransferase (3') type IIIa variants that inactivate amikacin but impose significant fitness costs.A panel of cytochrome P450 BM3 variants to produce drug metabolites and diversify lead compounds.Directed Evolution: Past, Present and Future.Assessing directed evolution methods for the generation of biosynthetic enzymes with potential in drug biosynthesis.Overcoming an optimization plateau in the directed evolution of highly efficient nerve agent bioscavengers.Controlled oxidation of remote sp3 C-H bonds in artemisinin via P450 catalysts with fine-tuned regio- and stereoselectivityThe influence of microbial physiology on biocatalyst activity and efficiency in the terminal hydroxylation of n-octane using Escherichia coli expressing the alkane hydroxylase, CYP153A6.Discovery of a regioselectivity switch in nitrating P450s guided by molecular dynamics simulations and Markov models.Constructing de novo biosynthetic pathways for chemical synthesis inside living cells.Directed enzyme evolution: climbing fitness peaks one amino acid at a time.Discovery of potent parthenolide-based antileukemic agents enabled by late-stage P450-mediated C-H functionalization.Controlling substrate specificity and product regio- and stereo-selectivities of P450 enzymes without mutagenesis.Enantioselective functionalization of allylic C-H bonds following a strategy of functionalization and diversification.P450-catalyzed intramolecular sp(3) C-H amination with arylsulfonyl azide substrates.Directed evolution: selection of the host organism.Functional soft materials from metallopolymers and metallosupramolecular polymers.P450(BM3) (CYP102A1): connecting the dots.Catalytic, mild, and selective oxyfunctionalization of linear alkanes: current challenges.Cytochromes P450 as promising catalysts for biotechnological application: chances and limitations.Cytochrome P450-Mediated Phytoremediation using Transgenic Plants: A Need for Engineered Cytochrome P450 Enzymes.
P2860
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P2860
Engineered alkane-hydroxylating cytochrome P450(BM3) exhibiting nativelike catalytic properties.
description
2007 nî lūn-bûn
@nan
2007年の論文
@ja
2007年学术文章
@wuu
2007年学术文章
@zh-cn
2007年学术文章
@zh-hans
2007年学术文章
@zh-my
2007年学术文章
@zh-sg
2007年學術文章
@yue
2007年學術文章
@zh
2007年學術文章
@zh-hant
name
Engineered alkane-hydroxylating cytochrome P450
@nl
Engineered alkane-hydroxylatin ...... tivelike catalytic properties.
@en
type
label
Engineered alkane-hydroxylating cytochrome P450
@nl
Engineered alkane-hydroxylatin ...... tivelike catalytic properties.
@en
prefLabel
Engineered alkane-hydroxylating cytochrome P450
@nl
Engineered alkane-hydroxylatin ...... tivelike catalytic properties.
@en
P50
P356
P1476
Engineered alkane-hydroxylatin ...... tivelike catalytic properties.
@en
P2093
Mike M Chen
P304
P356
10.1002/ANIE.200702616
P407
P577
2007-01-01T00:00:00Z