Systematic single-cell analysis of Pichia pastoris reveals secretory capacity limits productivity
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Fluorescent Reporter Libraries as Useful Tools for Optimizing Microbial Cell Factories: A Review of the Current Methods and ApplicationsCan too many copies spoil the broth?Stochastic particle barcoding for single-cell tracking and multiparametric analysis.Nanowell-based immunoassays for measuring single-cell secretion: characterization of transport and surface binding.Enhanced production of recombinant secretory proteins in Pichia pastoris by optimizing Kex2 P1' site.Translational arrest due to cytoplasmic redox stress delays adaptation to growth on methanol and heterologous protein expression in a typical fed-batch culture of Pichia pastoris.Rapid screening of cellular stress responses in recombinant Pichia pastoris strains using metabolite profiling.Gene and protein sequence optimization for high-level production of fully active and aglycosylated lysostaphin in Pichia pastoris.Expressing anti-HIV VRC01 antibody using the murine IgG1 secretion signal in Pichia pastoris.Pichia pastoris: protein production host and model organism for biomedical research.A systems biology approach to optimising hosts for industrial protein production.Microbial heterogeneity affects bioprocess robustness: dynamic single-cell analysis contributes to understanding of microbial populations.Liquid PTVA: a faster and cheaper alternative for generating multi-copy clones in Pichia pastoris.Quo vadis? The challenges of recombinant protein folding and secretion in Pichia pastoris.Following nature's roadmap: folding factors from plasma cells led to improvements in antibody secretion in S. cerevisiae.Production of Chimeric Acidic α-Amylase by the Recombinant Pichia pastoris and Its Applications.Characterization of a panARS-based episomal vector in the methylotrophic yeast Pichia pastoris for recombinant protein production and synthetic biology applications.Systematic Optimization of Protein Secretory Pathways in Saccharomyces cerevisiae to Increase Expression of Hepatitis B Small Antigen.Effects of glycerol supply and specific growth rate on methanol-free production of CALB by P. pastoris: functional characterisation of a novel promoter.A low-cost, multiplexable, automated flow cytometry procedure for the characterization of microbial stress dynamics in bioreactors.Cloning and expression of synthetic genes encoding the broad antimicrobial spectrum bacteriocins SRCAM 602, OR-7, E-760, and L-1077, by recombinant Pichia pastoris.Beyond the bulk: disclosing the life of single microbial cells.Co-expression of lipase isozymes for enhanced expression in Pichia pastoris.Effect of plasmid design and type of integration event on recombinant protein expression in Pichia pastoris.Going native: Direct high throughput screening of secreted full-length IgG antibodies against cell membrane proteins.Pichia pastoris X-33 has probiotic properties with remarkable antibacterial activity against Salmonella Typhimurium.Type III secretion as a generalizable strategy for the production of full-length biopolymer-forming proteins.Continuous flow micro-bioreactors for the production of biopharmaceuticals: the effect of geometry, surface texture, and flow rate.
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P2860
Systematic single-cell analysis of Pichia pastoris reveals secretory capacity limits productivity
description
2012 թուականին հրատարակուած գիտական յօդուած
@hyw
2012 թվականին հրատարակված գիտական հոդված
@hy
article publié dans la revue scientifique PLoS ONE
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artículu científicu espublizáu en 2012
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im Januar 2012 veröffentlichter wissenschaftlicher Artikel
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scientific article (publication date: 2012)
@en
vedecký článok (publikovaný 2012)
@sk
vědecký článek publikovaný v roce 2012
@cs
wetenschappelijk artikel (gepubliceerd in 2012)
@nl
наукова стаття, опублікована у 2012
@uk
name
Systematic single-cell analysi ...... y capacity limits productivity
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Systematic single-cell analysi ...... y capacity limits productivity
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Systematic single-cell analysi ...... y capacity limits productivity
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type
label
Systematic single-cell analysi ...... y capacity limits productivity
@ast
Systematic single-cell analysi ...... y capacity limits productivity
@en
Systematic single-cell analysi ...... y capacity limits productivity
@nl
prefLabel
Systematic single-cell analysi ...... y capacity limits productivity
@ast
Systematic single-cell analysi ...... y capacity limits productivity
@en
Systematic single-cell analysi ...... y capacity limits productivity
@nl
P2093
P2860
P3181
P1433
P1476
Systematic single-cell analysi ...... y capacity limits productivity
@en
P2093
J Christopher Love
Kerry Routenberg Love
Terrance A Stadheim
Timothy J Politano
Vasiliki Panagiotou
P2860
P304
P3181
P356
10.1371/JOURNAL.PONE.0037915
P407
P577
2012-01-01T00:00:00Z