Direct cloning, genetic engineering, and heterologous expression of the syringolin biosynthetic gene cluster in E. coli through Red/ET recombineering.
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Recent Advances in Understanding and Engineering Polyketide SynthesisMining the metabiome: identifying novel natural products from microbial communities.The distribution pattern of DNA and protoxin in Bacillus thuringiensis as revealed by laser confocal microscopy analysis.Genetic engineering and heterologous expression of the disorazol biosynthetic gene cluster via Red/ET recombineering.Room temperature electrocompetent bacterial cells improve DNA transformation and recombineering efficiency.Electroporation on microchips: the harmful effects of pH changes and scaling downReinvigorating natural product combinatorial biosynthesis with synthetic biology"Cre/loxP plus BAC": a strategy for direct cloning of large DNA fragment and its applications in Photorhabdus luminescens and Agrobacterium tumefaciens.An improved method for oriT-directed cloning and functionalization of large bacterial genomic regions.Simple and rapid direct cloning and heterologous expression of natural product biosynthetic gene cluster in Bacillus subtilis via Red/ET recombineering.Direct cloning and refactoring of a silent lipopeptide biosynthetic gene cluster yields the antibiotic taromycin A.The role of transcription in heterologous expression of polyketides in bacterial hosts.Improved seamless mutagenesis by recombineering using ccdB for counterselectionDirect Capture Technologies for Genomics-Guided Discovery of Natural Products.Unique marine derived cyanobacterial biosynthetic genes for chemical diversity.Novel Technologies for Optimal Strain Breeding.Native and engineered promoters in natural product discovery.High-titer heterologous production in E. coli of lyngbyatoxin, a protein kinase C activator from an uncultured marine cyanobacterium.Direct cloning and transplanting of large DNA fragments from Escherichia coli chromosome.RecET direct cloning and Redαβ recombineering of biosynthetic gene clusters, large operons or single genes for heterologous expression.Direct cloning and heterologous expression of the salinomycin biosynthetic gene cluster from Streptomyces albus DSM41398 in Streptomyces coelicolor A3(2).Rational and efficient site-directed mutagenesis of adenylation domain alters relative yields of luminmide derivatives in vivo.In vivo evidence for a prodrug activation mechanism during colibactin maturation.A Practical Guide to Recombineering in Photorhabdus and Xenorhabdus.Cloning of a copper resistance gene cluster from the cyanobacterium Synechocystis sp. PCC 6803 by recombineering recovery.ExoCET: exonuclease in vitro assembly combined with RecET recombination for highly efficient direct DNA cloning from complex genomes.TAR cloning and integrated overexpression of 6-demethylchlortetracycline biosynthetic gene cluster in Streptomyces aureofaciens.Heterologous production of glidobactins/luminmycins in Escherichia coli Nissle containing the glidobactin biosynthetic gene cluster from Burkholderia DSM7029.
P2860
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P2860
Direct cloning, genetic engineering, and heterologous expression of the syringolin biosynthetic gene cluster in E. coli through Red/ET recombineering.
description
2012 nî lūn-bûn
@nan
2012 թուականի Յուլիսին հրատարակուած գիտական յօդուած
@hyw
2012 թվականի հուլիսին հրատարակված գիտական հոդված
@hy
2012年の論文
@ja
2012年論文
@yue
2012年論文
@zh-hant
2012年論文
@zh-hk
2012年論文
@zh-mo
2012年論文
@zh-tw
2012年论文
@wuu
name
Direct cloning, genetic engine ...... through Red/ET recombineering.
@ast
Direct cloning, genetic engine ...... through Red/ET recombineering.
@en
Direct cloning, genetic engine ...... through Red/ET recombineering.
@nl
type
label
Direct cloning, genetic engine ...... through Red/ET recombineering.
@ast
Direct cloning, genetic engine ...... through Red/ET recombineering.
@en
Direct cloning, genetic engine ...... through Red/ET recombineering.
@nl
prefLabel
Direct cloning, genetic engine ...... through Red/ET recombineering.
@ast
Direct cloning, genetic engine ...... through Red/ET recombineering.
@en
Direct cloning, genetic engine ...... through Red/ET recombineering.
@nl
P2093
P2860
P356
P1433
P1476
Direct cloning, genetic engine ...... through Red/ET recombineering
@en
P2093
Francis A Stewart
Youming Zhang
P2860
P304
P356
10.1002/CBIC.201200310
P577
2012-07-31T00:00:00Z