Development of an optimized expression system for the screening of antibody libraries displayed on the Escherichia coli surface.
about
Intrabody and Parkinson's diseaseComputational redesign of the lipid-facing surface of the outer membrane protein OmpA.Strategies for successful recombinant expression of disulfide bond-dependent proteins in Escherichia coli.Isolation of high-affinity ligand-binding proteins by periplasmic expression with cytometric screening (PECS).Quantitative analysis of the effect of the mutation frequency on the affinity maturation of single chain Fv antibodies.Efficient isolation of soluble intracellular single-chain antibodies using the twin-arginine translocation machinery.Flow-cytometric isolation of human antibodies from a nonimmune Saccharomyces cerevisiae surface display library.Function-based isolation of novel enzymes from a large library.Anchored periplasmic expression, a versatile technology for the isolation of high-affinity antibodies from Escherichia coli-expressed libraries.Rapid isolation of high-affinity protein binding peptides using bacterial display.Bacterial display using circularly permuted outer membrane protein OmpX yields high affinity peptide ligandsOptimizing the affinity and specificity of proteins with molecular display.Design of synthetic antibody libraries.Directed evolution of a biterminal bacterial display scaffold enhances the display of diverse peptidesE-clonal antibodies: selection of full-length IgG antibodies using bacterial periplasmic display.Botulinum neurotoxins and botulism: a novel therapeutic approach.A bacteria colony-based screen for optimal linker combinations in genetically encoded biosensorsSelf-made phage libraries with heterologous inserts in the Mtd of Bordetella bronchiseptica.Phage display and pharmacogenomics.Characterization of an inducible promoter in different DNA copy number conditions.Immobilization of cells with surface-displayed chitin-binding domainCell surface display yields evolvable, clickable antibody fragments.Antibody isolation from immunized animals: comparison of phage display and antibody discovery via V gene repertoire mining.Selection of single domain antibodies from immune libraries displayed on the surface of E. coli cells with two β-domains of opposite topologies.Development trends for generation of single-chain antibody fragments.Rapid isolation of antibody from a synthetic human antibody library by repeated fluorescence-activated cell sorting (FACS)An engineered autotransporter-based surface expression vector enables efficient display of Affibody molecules on OmpT-negative E. coli as well as protease-mediated secretion in OmpT-positive strains.A gateway-based system for fast evaluation of protein-protein interactions in bacteria.A switchable yeast display/secretion systemOptimizing antibody expression by using the naturally occurring framework diversity in a live bacterial antibody display systemInvestigating affinity-maturation strategies and reproducibility of fluorescence-activated cell sorting using a recombinant ADAPT library displayed on staphylococci.Transpo-mAb display: Transposition-mediated B cell display and functional screening of full-length IgG antibody libraries.Engineered bacterial outer membrane vesicles with enhanced functionalityNovel bacterial surface display systems based on outer membrane anchoring elements from the marine bacterium Vibrio anguillarum.Anti-human fibroblast growth factor-21 monoclonal antibody preparation, characterization and analysis of in vitro bioactivity.Use of Pseudomonas putida EstA as an anchoring motif for display of a periplasmic enzyme on the surface of Escherichia coliThe application of Tet repressor in prokaryotic gene regulation and expressionBiotechnological applications of recombinant single-domain antibody fragments.Display of passenger proteins on the surface of Escherichia coli K-12 by the enterohemorrhagic E. coli intimin EaeA.Assembly of G protein-coupled receptors onto nanosized bacterial magnetic particles using Mms16 as an anchor molecule.
P2860
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P2860
Development of an optimized expression system for the screening of antibody libraries displayed on the Escherichia coli surface.
description
1999 nî lūn-bûn
@nan
1999 թուականի Յուլիսին հրատարակուած գիտական յօդուած
@hyw
1999 թվականի հուլիսին հրատարակված գիտական հոդված
@hy
1999年の論文
@ja
1999年論文
@yue
1999年論文
@zh-hant
1999年論文
@zh-hk
1999年論文
@zh-mo
1999年論文
@zh-tw
1999年论文
@wuu
name
Development of an optimized ex ...... the Escherichia coli surface.
@ast
Development of an optimized ex ...... the Escherichia coli surface.
@en
type
label
Development of an optimized ex ...... the Escherichia coli surface.
@ast
Development of an optimized ex ...... the Escherichia coli surface.
@en
prefLabel
Development of an optimized ex ...... the Escherichia coli surface.
@ast
Development of an optimized ex ...... the Escherichia coli surface.
@en
P2093
P2860
P356
P1476
Development of an optimized ex ...... n the Escherichia coli surface
@en
P2093
B L Iverson
G Georgiou
P S Daugherty
P2860
P304
P356
10.1093/PROTEIN/12.7.613
P577
1999-07-01T00:00:00Z