Molecular evolution by staggered extension process (StEP) in vitro recombination. - Wikidata - wikimedia - TriplyDB

Molecular evolution by staggered extension process (StEP) in vitro recombination.

Molecular evolution by staggered extension process (StEP) in vitro recombination.

http://www.wikidata.org/entity/Q32075357

about

DNA polymerase hybrids derived from the family-B enzymes of Pyrococcus furiosus and Thermococcus kodakarensis: improving performance in the polymerase chain reaction Ultrahigh-throughput screening in drop-based microfluidics for directed evolution.Directed evolution of adeno-associated virus to an infectious respiratory virus In vitro evolution of single-chain antibodies using mRNA display Offset recombinant PCR: a simple but effective method for shuffling compact heterologous domains.Directed evolution strategies for improved enzymatic performance Nucleotide exchange and excision technology (NExT) DNA shuffling: a robust method for DNA fragmentation and directed evolution To get what we aim for--progress in diversity generation methods Molecular Engineering of Organophosphate Hydrolysis Activity from a Weak Promiscuous Lactonase Template Directed evolution converts subtilisin E into a functional equivalent of thermitase Directed evolution: tailoring biocatalysts for industrial applications The protein folding problem Directed evolution of a G protein-coupled receptor for expression, stability, and binding selectivity Biocatalyst development by directed evolution Molecular breeding of polymerases for amplification of ancient DNA In vitro template-change PCR to create single crossover libraries: a case study with B. thuringiensis Cry2A toxins Directed evolution of new catalytic activity using the alpha/beta-barrel scaffold.Computationally mapping sequence space to understand evolutionary protein engineering.In vitro rapid evolution of fungal immunomodulatory proteins by DNA family shuffling.DNA shuffling method for generating highly recombined genes and evolved enzymes.A novel, high performance enzyme for starch liquefaction. Discovery and optimization of a low pH, thermostable alpha-amylase.Investigating and Engineering Enzymes by Genetic Selection.Chemical and biochemical strategies for the randomization of protein encoding DNA sequences: library construction methods for directed evolution.Generation of a broad esterolytic subtilisin using combined molecular evolution and periplasmic expression.Generating segmental mutations in haloalkane dehalogenase: a novel part in the directed evolution toolbox.Conflicting requirements of Plasmodium falciparum dihydrofolate reductase mutations conferring resistance to pyrimethamine-WR99210 combination.Novel ceftazidime-resistance beta-lactamases generated by a codon-based mutagenesis method and selection.A general method for scanning unnatural amino acid mutagenesis.Library analysis of SCHEMA-guided protein recombination.Rapid generation of incremental truncation libraries for protein engineering using alpha-phosphothioate nucleotides.Libraries of hybrid proteins from distantly related sequences.Directed evolution of novel polymerase activities: mutation of a DNA polymerase into an efficient RNA polymerase.eCodonOpt: a systematic computational framework for optimizing codon usage in directed evolution experiments Thermostabilization of cellulosomal endoglucanase EngB from Clostridium cellulovorans by in vitro DNA recombination with non-cellulosomal endoglucanase EngD.Assembly of designed oligonucleotides as an efficient method for gene recombination: a new tool in directed evolution.Using an AraC-based three-hybrid system to detect biocatalysts in vivo.Ribosome display efficiently selects and evolves high-affinity antibodies in vitro from immune libraries.Improving the carboligase activity of benzoylformate decarboxylase from Pseudomonas putida by a combination of directed evolution and site-directed mutagenesis.Creation of the first anomeric D/L-sugar kinase by means of directed evolution Rapid evolution of beta-glucuronidase specificity by saturation mutagenesis of an active site loop.

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P2860

Molecular evolution by staggered extension process (StEP) in vitro recombination.

http://www.wikidata.org/entity/Q32075357

description

1998 nî lūn-bûn

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1998 թուականի Մարտին հրատարակուած գիտական յօդուած

@hyw

1998 թվականի մարտին հրատարակված գիտական հոդված

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1998年の論文

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1998年論文

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1998年論文

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1998年論文

@zh-hk

1998年論文

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1998年論文

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1998年论文

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name

Molecular evolution by staggered extension process (StEP) in vitro recombination.

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Molecular evolution by staggered extension process (StEP) in vitro recombination.

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Molecular evolution by staggered extension process (StEP) in vitro recombination.

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Molecular evolution by staggered extension process (StEP) in vitro recombination.

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prefLabel

Molecular evolution by staggered extension process (StEP) in vitro recombination.

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Molecular evolution by staggered extension process (StEP) in vitro recombination.

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Nature Biotechnology

P1476

Molecular evolution by staggered extension process (StEP) in vitro recombination.

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P2093

Affholter JA

http://www.w3.org/2001/XMLSchema#string

Arnold FH

http://www.w3.org/2001/XMLSchema#string

Giver L

http://www.w3.org/2001/XMLSchema#string

Shao Z

http://www.w3.org/2001/XMLSchema#string

Zhao H

http://www.w3.org/2001/XMLSchema#string

P2860

Rapid evolution of a protein in vitro by DNA shuffling

Directed evolution of enzyme catalysts

Random-priming in vitro recombination: an effective tool for directed evolution.

Functional and nonfunctional mutations distinguished by random recombination of homologous genes.

Optimization of DNA shuffling for high fidelity recombination.

Sequence analysis of the HLA-DR beta and HLA-DQ beta loci from three Pemphigus vulgaris patients.

Strategies for the in vitro evolution of protein function: enzyme evolution by random recombination of improved sequences.

P2888

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258-261

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scholarly article

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10.1038/NBT0398-258

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3

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16

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51320475

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1017776064

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9528005

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