Overexpression of mammalian integral membrane proteins for structural studies.
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Heterologous expression and purification systems for structural proteomics of mammalian membrane proteinsMembrane protein structure determination - the next generationOverexpression of membrane proteins in mammalian cells for structural studiesCrystal Structure of a Thermally Stable Rhodopsin MutantComparative lipidomics in clinical isolates of Candida albicans reveal crosstalk between mitochondria, cell wall integrity and azole resistanceCharge asymmetry in the proteins of the outer membrane.Progress in the analysis of membrane protein structure and function.Structures of membrane proteins.Overcoming barriers to membrane protein structure determination.The use of phage display in the study of receptors and their ligands.A novel method of production and biophysical characterization of the catalytic domain of yeast oligosaccharyl transferaseTuning microbial hosts for membrane protein production.Comparative lipidomics of azole sensitive and resistant clinical isolates of Candida albicans reveals unexpected diversity in molecular lipid imprints.Heterologous overexpression and mutagenesis of the human bile salt export pump (ABCB11) using DREAM (Directed REcombination-Assisted Mutagenesis).Detergent screening of the human voltage-gated proton channel using fluorescence-detection size-exclusion chromatography.Engineering cells to improve protein expression.Employing Escherichia coli to functionally express, purify, and characterize a human transporterHeterologous expression of membrane proteins: choosing the appropriate host.Design, expression, and purification of de novo transmembrane "hairpin" peptides.Characterization of a functional bacterial homologue of sodium-dependent neurotransmitter transporters.Transient and stable expression of the neurotensin receptor NTS1: a comparison of the baculovirus-insect cell and the T-REx-293 expression systems.Crystal structures of fusion proteins with large-affinity tags.Making water-soluble integral membrane proteins in vivo using an amphipathic protein fusion strategy.MiRNA mimic screen for improved expression of functional neurotensin receptor from HEK293 cellsEffects of additives on surfactant phase behavior relevant to bacteriorhodopsin crystallizationEnhancing functional production of G protein-coupled receptors in Pichia pastoris to levels required for structural studies via a single expression screenConformational thermostabilization of the beta1-adrenergic receptor in a detergent-resistant form.Isotope labeling for solution and solid-state NMR spectroscopy of membrane proteinsEfficient cell-free production of olfactory receptors: detergent optimization, structure, and ligand binding analyses.Toward structural elucidation of the gamma-secretase complex.Tricks of the trade used to accelerate high-resolution structure determination of membrane proteins.Recombinant production of the human aquaporins in the yeast Pichia pastoris (Invited Review).Further advances in the production of membrane proteins in Pichia pastoris.Crystallization of Membrane Proteins: An Overview.Current strategies for protein production and purification enabling membrane protein structural biology.Quality control in eukaryotic membrane protein overproduction.Overexpressing human membrane proteins in stably transfected and clonal human embryonic kidney 293S cellsHigh-level expression and purification of Cys-loop ligand-gated ion channels in a tetracycline-inducible stable mammalian cell line: GABAA and serotonin receptorsPurification and mass spectroscopic analysis of human CB1 cannabinoid receptor functionally expressed using the baculovirus system.Cell-free production and characterisation of human uncoupling protein 1-3.
P2860
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P2860
Overexpression of mammalian integral membrane proteins for structural studies.
description
2001 nî lūn-bûn
@nan
2001 թուականի Օգոստոսին հրատարակուած գիտական յօդուած
@hyw
2001 թվականի օգոստոսին հրատարակված գիտական հոդված
@hy
2001年の論文
@ja
2001年論文
@yue
2001年論文
@zh-hant
2001年論文
@zh-hk
2001年論文
@zh-mo
2001年論文
@zh-tw
2001年论文
@wuu
name
Overexpression of mammalian integral membrane proteins for structural studies.
@ast
Overexpression of mammalian integral membrane proteins for structural studies.
@en
Overexpression of mammalian integral membrane proteins for structural studies.
@nl
type
label
Overexpression of mammalian integral membrane proteins for structural studies.
@ast
Overexpression of mammalian integral membrane proteins for structural studies.
@en
Overexpression of mammalian integral membrane proteins for structural studies.
@nl
prefLabel
Overexpression of mammalian integral membrane proteins for structural studies.
@ast
Overexpression of mammalian integral membrane proteins for structural studies.
@en
Overexpression of mammalian integral membrane proteins for structural studies.
@nl
P2860
P1433
P1476
Overexpression of mammalian integral membrane proteins for structural studies
@en
P2093
P2860
P356
10.1016/S0014-5793(01)02711-9
P407
P577
2001-08-01T00:00:00Z