In vitro aging of calmodulin generates isoaspartate at multiple Asn-Gly and Asp-Gly sites in calcium-binding domains II, III, and IV
about
Complex interactions of the protein L-isoaspartyl methyltransferase and calmodulin revealed with the yeast two-hybrid systemSimultaneous assessment of Asp isomerization and Asn deamidation in recombinant antibodies by LC-MS following incubation at elevated temperaturesSelective degradation of oxidized calmodulin by the 20 S proteasome.Deamidation of human proteins.Selective cleavage of isoaspartyl peptide bonds by hydroxylamine after methyltransferase priming.Mildly acidic conditions eliminate deamidation artifact during proteolysis: digestion with endoprotease Glu-C at pH 4.5.Post-translational aging of proteins in osteoarthritic cartilage and synovial fluid as measured by isomerized aspartateBrain proteomics supports the role of glutamate metabolism and suggests other metabolic alterations in protein l-isoaspartyl methyltransferase (PIMT)-knockout miceDifferentiation of isomeric amino acid residues in proteins and peptides using mass spectrometry.Isoaspartate in ribosomal protein S11 of Escherichia coli.Identification of succinimide sites in proteins by N-terminal sequence analysis after alkaline hydroxylamine cleavage.Mapping site-specific changes that affect stability of the N-terminal domain of calmodulin.Use of 18O labels to monitor deamidation during protein and peptide sample processing.Transgenic expression of the protein-L-isoaspartyl methyltransferase (PIMT) gene in the brain rescues mice from the fatal epilepsy of PIMT deficiency.Isoaspartate in chrondroitin sulfate proteoglycans of mammalian brain.Protein L-isoaspartyl methyltransferase catalyzes in vivo racemization of Aspartate-25 in mammalian histone H2B.Synapsin I is a major endogenous substrate for protein L-isoaspartyl methyltransferase in mammalian brain.Ca2+-free calmodulin and calmodulin damaged by in vitro aging are selectively degraded by 26 S proteasomes without ubiquitination.Carboxyl methylation of deamidated calmodulin increases its stability in Xenopus oocyte cytoplasm. Implications for protein repair.
P2860
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P2860
In vitro aging of calmodulin generates isoaspartate at multiple Asn-Gly and Asp-Gly sites in calcium-binding domains II, III, and IV
description
1993 nî lūn-bûn
@nan
1993年の論文
@ja
1993年学术文章
@wuu
1993年学术文章
@zh-cn
1993年学术文章
@zh-hans
1993年学术文章
@zh-my
1993年学术文章
@zh-sg
1993年學術文章
@yue
1993年學術文章
@zh
1993年學術文章
@zh-hant
name
In vitro aging of calmodulin g ...... inding domains II, III, and IV
@ast
In vitro aging of calmodulin g ...... inding domains II, III, and IV
@en
type
label
In vitro aging of calmodulin g ...... inding domains II, III, and IV
@ast
In vitro aging of calmodulin g ...... inding domains II, III, and IV
@en
prefLabel
In vitro aging of calmodulin g ...... inding domains II, III, and IV
@ast
In vitro aging of calmodulin g ...... inding domains II, III, and IV
@en
P2860
P356
P1433
P1476
In vitro aging of calmodulin g ...... inding domains II, III, and IV
@en
P2093
P2860
P304
P356
10.1002/PRO.5560021011
P577
1993-10-01T00:00:00Z