Isolation of picomolar affinity anti-c-erbB-2 single-chain Fv by molecular evolution of the complementarity determining regions in the center of the antibody binding site.
about
Procaryotic expression of single-chain variable-fragment (scFv) antibodies: secretion in L-form cells of Proteus mirabilis leads to active product and overcomes the limitations of periplasmic expression in Escherichia coliAffinity and avidity in antibody-based tumor targetingEliminating helper phage from phage displayProlonged in vivo tumour retention of a human diabody targeting the extracellular domain of human HER2/neuConstruction and high cytoplasmic expression of a tumoricidal single-chain antibody against hepatocellular carcinomaImplications of receptor-mediated endocytosis and intracellular trafficking dynamics in the development of antibody drug conjugatesStructure of an anti-blood group A Fv and improvement of its binding affinity without loss of specificityMolecular evolution of antibody cross-reactivity for two subtypes of type A botulinum neurotoxinX-ray structures of a hydrolytic antibody and of complexes elucidate catalytic pathway from substrate binding and transition state stabilization through water attack and product releaseIgE immunotherapy against cancerAffinity maturation of antibodies assisted by in silico modeling.Recombinant human factor VIII-specific affinity ligands selected from phage-displayed combinatorial libraries of protein A.Biological libraries.Quantitative analysis of the effect of the mutation frequency on the affinity maturation of single chain Fv antibodies.Affinity maturation of Cry1Aa toxin to the Bombyx mori cadherin-like receptor by directed evolution.Affinity maturation of Fab antibody fragments by fluorescent-activated cell sorting of yeast-displayed libraries.Ribosome display efficiently selects and evolves high-affinity antibodies in vitro from immune libraries.Selection of recombinant, library-derived antibody fragments against p24 for human immunodeficiency virus type 1 diagnostics.A general method for greatly improving the affinity of antibodies by using combinatorial librariesOptimizing the affinity and specificity of proteins with molecular display.A simple vector system to improve performance and utilisation of recombinant antibodiesAn affinity-enhanced neutralizing antibody against the membrane-proximal external region of human immunodeficiency virus type 1 gp41 recognizes an epitope between those of 2F5 and 4E10Design of synthetic antibody libraries.Affinity-matured recombinant antibody fragments analyzed by single-molecule force spectroscopyDevelopment and implementation of a single-chain Fv antibody for specific detection of Bacillus anthracis spores.An scFv intrabody against the nonamyloid component of alpha-synuclein reduces intracellular aggregation and toxicityMimotopes and proteome analyses using human genomic and cDNA epitope phage display.Specificity tuning of antibody fragments to neutralize two human chemokines with a single agentAffinity maturation of human botulinum neurotoxin antibodies by light chain shuffling via yeast mating.Characterization of an engineered human purine nucleoside phosphorylase fused to an anti-her2/neu single chain Fv for use in ADEPTSelection of full-length IgGs by tandem display on filamentous phage particles and Escherichia coli fluorescence-activated cell sorting screening.A novel human anti-interleukin-1β neutralizing monoclonal antibody showing in vivo efficacyMining human antibody repertoires.Applications of recombinant antibodies in plant pathology.The use of biosensor technology for the engineering of antibodies and enzymes.Antibody buffering of a ligand in vivoProduction and characterization of a recombinant single-chain antibody against Hantaan virus envelop glycoprotein.Beyond natural antibodies: the power of in vitro display technologies.High scFv-receptor affinity does not enhance the antitumor activity of HER2-retargeted measles virus.Targeting of mesenchymal stem cells to ovarian tumors via an artificial receptor.
P2860
Q24550594-D1091B5A-64EB-43B4-911C-10E67C8ADF21Q24613552-3F092CA6-2362-446A-8E46-4B5E5E62B55DQ24674603-3DE8DFFC-233F-4CAA-AB56-7D566BD60494Q24685875-6DD28AB3-F8C5-4E49-83AF-0F88ED429599Q24804173-11EE98A5-8D38-43CE-B924-1F94FF40CF2AQ27007486-7BF2978C-6F24-4DEE-91E8-B77C5C5E11EBQ27635579-2A719F36-751B-42E2-85FD-601DA15D038BQ27643327-BA8004E4-FCBB-44C8-9F30-53D1ECB2E860Q27740546-9CD5A5CB-F482-43D2-B514-C3CDE32966C4Q28081223-313BA60E-DBBA-43EF-A0FA-508F0497CD8CQ30482656-030A7525-C64A-4730-BF7E-CCFA08F4B4C4Q30698098-9375BFA0-69A5-4951-A9C7-FE6756F33DB0Q30796540-25B08984-AA76-4125-8790-AA7AD29B1969Q30841593-127CC627-550E-4AD1-A8B9-18CA103D7B44Q31109314-036D843A-EFED-4189-9C17-D205C23C08CCQ31148151-CEEDDA0D-8472-4A91-9E35-2676C1A435C8Q31967754-B0CBF9BB-06E8-4953-ABE4-0ABA0DD5F8E5Q32026627-3E7D3711-269A-43F2-91B9-1CD7EDEFFDC8Q33216580-EDC0673A-F764-42BB-89E3-0D1ED056B330Q33252425-B03C6DD7-4491-4142-BD5E-5BD5AAC49096Q33265933-EEABB4A2-4C29-4FE3-BBEE-2DE9B997AFA5Q33272711-C643B78F-C268-478E-ABAC-EC701D86BA1CQ33283622-14FFB301-348F-421D-9531-942667D3E3CDQ33293045-7306713F-E44C-45FE-8A00-E8497BF32776Q33304155-2200D41F-1CA6-4E3D-A5AE-7B6634D1CD1BQ33317678-6FEEB7EF-C4AA-4E8F-9BC8-4C8C7C47DA9EQ33351956-AE7F1242-756E-484C-A803-B3D8D1B9201CQ33523566-8E542A51-7B47-47DF-B938-F3B5A68964B8Q33531378-41EF417F-FAE3-4A80-A23A-8F61F065B899Q33562665-4C1F1C42-91F5-4DED-9CBA-F626D0A7D4FEQ33566182-73EEBD10-C156-45D2-B69B-68E16E32FC50Q33573612-9C78E32A-4F3A-4241-A492-6F7B4BCAB889Q33588489-0899D681-A5CC-4376-B863-8F2E249E2886Q33612876-847D0693-4548-4AA5-8DE7-850DCDBA4BC2Q33682947-96A92128-1EA0-46B5-B12C-453741FA688EQ33715292-0DD11C1D-757E-47E6-AE60-0102EED5DD23Q33751225-112A0C0E-8371-4A10-8624-12E03471115CQ33841864-B62D11D9-5E4F-418C-9001-B67B00FD3525Q33896979-371B7559-95E1-417F-B621-548235FF8CA0Q33907262-ED283CF4-F43F-4E2A-B868-BE79BE188BD1
P2860
Isolation of picomolar affinity anti-c-erbB-2 single-chain Fv by molecular evolution of the complementarity determining regions in the center of the antibody binding site.
description
1996 nî lūn-bûn
@nan
1996年の論文
@ja
1996年論文
@yue
1996年論文
@zh-hant
1996年論文
@zh-hk
1996年論文
@zh-mo
1996年論文
@zh-tw
1996年论文
@wuu
1996年论文
@zh
1996年论文
@zh-cn
name
Isolation of picomolar affinit ...... of the antibody binding site.
@en
type
label
Isolation of picomolar affinit ...... of the antibody binding site.
@en
prefLabel
Isolation of picomolar affinit ...... of the antibody binding site.
@en
P2093
P356
P1476
Isolation of picomolar affinit ...... of the antibody binding site.
@en
P2093
Crawford RS
Marshall KW
P304
P356
10.1006/JMBI.1996.0598
P407
P577
1996-11-01T00:00:00Z