Quantitative analysis of the effect of the mutation frequency on the affinity maturation of single chain Fv antibodies.
about
On the conservative nature of intragenic recombinationThermodynamic prediction of protein neutralityCompensatory mutations cause excess of antagonistic epistasis in RNA secondary structure foldingA dual-fluorescence reporter system for high-throughput clone characterization and selection by cell sortingIn Vitro Selection of Cancer Cell-Specific Molecular Recognition Elements from Amino Acid LibrariesAntibody recognition of a unique tumor-specific glycopeptide antigenLethal mutagenesis of bacteriaComputationally mapping sequence space to understand evolutionary protein engineering.Enzyme improvement in the absence of structural knowledge: a novel statistical approach.Preparation of functional single-chain antibodies against bioactive gibberellins by utilizing randomly mutagenized phage-display libraries.Function-based isolation of novel enzymes from a large library.Tailoring in vitro evolution for protein affinity or stability.A central core structure in an antibody variable domain determines antigen specificity.Shuffled antibody libraries created by in vivo homologous recombination and yeast surface display.Genetic analysis of the twin arginine translocator secretion pathway in bacteria.Anchored periplasmic expression, a versatile technology for the isolation of high-affinity antibodies from Escherichia coli-expressed libraries.Improvements to the Kunkel mutagenesis protocol for constructing primary and secondary phage-display libraries.Cell-Surface display of heterologous proteins: From high-throughput screening to environmental applications.Rapid isolation of high-affinity protein binding peptides using bacterial display.Affinity maturation of Fab antibody fragments by fluorescent-activated cell sorting of yeast-displayed libraries.Fine tuning of the specificity of an anti-progesterone antibody by first and second sphere residue engineering.Recombinant antibodies: a novel approach to cancer diagnosis and therapy.Phage shock protein PspA of Escherichia coli relieves saturation of protein export via the Tat pathway.A general method for greatly improving the affinity of antibodies by using combinatorial librariesDirected evolution of adeno-associated virus yields enhanced gene delivery vectors.Optimizing the affinity and specificity of proteins with molecular display.A scFv antibody mutant isolated in a genetic screen for improved export via the twin arginine transporter pathway exhibits faster folding.Design of synthetic antibody libraries.Impact of single-chain Fv antibody fragment affinity on nanoparticle targeting of epidermal growth factor receptor-expressing tumor cells.A focused antibody library for selecting scFvs expressed at high levels in the cytoplasm.Directed evolution of a biterminal bacterial display scaffold enhances the display of diverse peptidesDNA shuffling of adeno-associated virus yields functionally diverse viral progenyAffinity maturation generates greatly improved xyloglucan-specific carbohydrate binding modulesDirected evolution of an anti-human red blood cell antibodyAttainment of 15-fold higher affinity of a Fusarium-specific single-chain antibody by directed molecular evolution coupled to phage display.Passive protection against anthrax by using a high-affinity antitoxin antibody fragment lacking an Fc regionMimicking the germinal center reaction in hybridoma cells to isolate temperature-selective anti-PEG antibodies.Engineering a large protein by combined rational and random approaches: stabilizing the Clostridium thermocellum cellobiose phosphorylase.Cell surface display yields evolvable, clickable antibody fragments.Predicting the tolerance of proteins to random amino acid substitution.
P2860
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P2860
Quantitative analysis of the effect of the mutation frequency on the affinity maturation of single chain Fv antibodies.
description
2000 nî lūn-bûn
@nan
2000 թուականի Փետրուարին հրատարակուած գիտական յօդուած
@hyw
2000 թվականի փետրվարին հրատարակված գիտական հոդված
@hy
2000年の論文
@ja
2000年論文
@yue
2000年論文
@zh-hant
2000年論文
@zh-hk
2000年論文
@zh-mo
2000年論文
@zh-tw
2000年论文
@wuu
name
Quantitative analysis of the e ...... of single chain Fv antibodies.
@ast
Quantitative analysis of the e ...... of single chain Fv antibodies.
@en
type
label
Quantitative analysis of the e ...... of single chain Fv antibodies.
@ast
Quantitative analysis of the e ...... of single chain Fv antibodies.
@en
prefLabel
Quantitative analysis of the e ...... of single chain Fv antibodies.
@ast
Quantitative analysis of the e ...... of single chain Fv antibodies.
@en
P2093
P2860
P356
P1476
Quantitative analysis of the e ...... of single chain Fv antibodies.
@en
P2093
B L Iverson
G Georgiou
P S Daugherty
P2860
P304
P356
10.1073/PNAS.030527597
P407
P577
2000-02-01T00:00:00Z