Directed enzyme evolution: climbing fitness peaks one amino acid at a time.
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Predictability of evolutionary trajectories in fitness landscapesComputational design of Candida boidinii xylose reductase for altered cofactor specificity"Fluctuograms" reveal the intermittent intra-protein communication in subtilisin Carlsberg and correlate mechanical coupling with co-evolutionIdentification and analysis of residues contained on β → α loops of the dual-substrate (βα)8phosphoribosyl isomerase A specific for its phosphoribosyl anthranilate isomerase activityMolecular bases of cyclodextrin adapter interactions with engineered protein nanoporesExpanding the Nucleotide and Sugar 1-Phosphate Promiscuity of Nucleotidyltransferase RmlA via Directed EvolutionAzurin as a Protein Scaffold for a Low-coordinate Nonheme Iron Site with a Small-molecule Binding PocketDetermination of the Structure of the Catabolic N-Succinylornithine Transaminase (AstC) from Escherichia coliStructural and dynamic changes associated with beneficial engineered single-amino-acid deletion mutations in enhanced green fluorescent proteinDirected divergent evolution of a thermostable D-tagatose epimerase towards improved activity for two hexose substratesMicrobial engineering for the production of advanced biofuelsDirected evolution: tailoring biocatalysts for industrial applicationsStrategies for discovery and improvement of enzyme function: state of the art and opportunitiesThe single substitution I259T, conserved in the plasminogen activator Pla of pandemic Yersinia pestis branches, enhances fibrinolytic activity.Cis-suppression to arrest protein aggregation in mammalian cells.Controlling enzyme inhibition using an expanded set of genetically encoded amino acids.The quest for molecular quasi-species in ligand-activity space and its application to directed enzyme evolution.Directed evolution strategies for enantiocomplementary haloalkane dehalogenases: from chemical waste to enantiopure building blocks.The architecture of a prototypical bacterial signaling circuit enables a single point mutation to confer novel network propertiesEvolving thermostability in mutant libraries of ligninolytic oxidoreductases expressed in yeast.Engineering monolignol 4-O-methyltransferases to modulate lignin biosynthesisEngineering Nucleotide Specificity of Succinyl-CoA Synthetase in Blastocystis: The Emerging Role of Gatekeeper ResiduesInferring fitness landscapes by regression produces biased estimates of epistasis.Engineering Kinases to Phosphorylate Nucleoside Analogs for Antiviral and Cancer Therapy.The contribution of coevolving residues to the stability of KDO8P synthase.Applying neutral drift to the directed molecular evolution of a β-glucuronidase into a β-galactosidase: Two different evolutionary pathways lead to the same variant.A moth pheromone brewery: production of (Z)-11-hexadecenol by heterologous co-expression of two biosynthetic genes from a noctuid moth in a yeast cell factory.Experimental evolution of adenylate kinase reveals contrasting strategies toward protein thermostability.Overview of regulatory strategies and molecular elements in metabolic engineering of bacteria.Engineering a large protein by combined rational and random approaches: stabilizing the Clostridium thermocellum cellobiose phosphorylase.Engineering synthetic recursive pathways to generate non-natural small molecules.Intramolecular epistasis and the evolution of a new enzymatic functionIdentification of human butyrylcholinesterase organophosphate-resistant variants through a novel mammalian enzyme functional screen.Increasing activity and thermal resistance of Bacillus gibsonii alkaline protease (BgAP) by directed evolution.Replaying the tape of life: quantification of the predictability of evolution.Polyspecific pyrrolysyl-tRNA synthetases from directed evolutionApplying physics-based scoring to calculate free energies of binding for single amino acid mutations in protein-protein complexesDirected evolution of fungal laccasesA synthetic system links FeFe-hydrogenases to essential E. coli sulfur metabolismMutagenic Organized Recombination Process by Homologous IN vivo Grouping (MORPHING) for directed enzyme evolution.
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Directed enzyme evolution: climbing fitness peaks one amino acid at a time.
description
article científic
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article scientifique
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articolo scientifico
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artigo científico
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bilimsel makale
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scientific article published on 25 February 2009
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vedecký článok
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vetenskaplig artikel
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videnskabelig artikel
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vědecký článek
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name
Directed enzyme evolution: climbing fitness peaks one amino acid at a time.
@en
Directed enzyme evolution: climbing fitness peaks one amino acid at a time.
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type
label
Directed enzyme evolution: climbing fitness peaks one amino acid at a time.
@en
Directed enzyme evolution: climbing fitness peaks one amino acid at a time.
@nl
prefLabel
Directed enzyme evolution: climbing fitness peaks one amino acid at a time.
@en
Directed enzyme evolution: climbing fitness peaks one amino acid at a time.
@nl
P2860
P1476
Directed enzyme evolution: climbing fitness peaks one amino acid at a time.
@en
P2093
Cara A Tracewell
P2860
P356
10.1016/J.CBPA.2009.01.017
P577
2009-02-25T00:00:00Z