An optimized electrofusion-based protocol for generating virus-specific human monoclonal antibodies
about
Stable long-term cultures of self-renewing B cells and their applicationsImmunogenicity of membrane-bound HIV-1 gp41 membrane-proximal external region (MPER) segments is dominated by residue accessibility and modulated by stereochemistry.Neutralizing antibodies derived from the B cells of 1918 influenza pandemic survivors.A broadly neutralizing human monoclonal antibody that recognizes a conserved, novel epitope on the globular head of the influenza H1N1 virus hemagglutinin.Human monoclonal antibodies to pandemic 1957 H2N2 and pandemic 1968 H3N2 influenza virusesMonoclonal antibodies to DIPA: a novel binding partner of p120-catenin isoform 1Mining human antibody repertoires.Broadly neutralizing antibodies with few somatic mutations and hepatitis C virus clearanceA novel pre-fusion conformation-specific neutralizing epitope on the respiratory syncytial virus fusion protein.Mechanism of human antibody-mediated neutralization of Marburg virusHarnessing the immune system's arsenal: producing human monoclonal antibodies for therapeutics and investigating immune responses.Epitope-specific human influenza antibody repertoires diversify by B cell intraclonal sequence divergence and interclonal convergenceRescue of HIV-1 broad neutralizing antibody-expressing B cells in 2F5 VH x VL knockin mice reveals multiple tolerance controls.Analysis of a clonal lineage of HIV-1 envelope V2/V3 conformational epitope-specific broadly neutralizing antibodies and their inferred unmutated common ancestors.Recovery of a human natural antibody against the noncollagenous-1 domain of type IV collagen using humanized models.Enhanced outgrowth of EBV-transformed chronic lymphocytic leukemia B cells mediated by coculture with macrophage feeder cells.Generation of Recombinant Monoclonal Antibodies from Immunised Mice and Rabbits via Flow Cytometry and Sorting of Antigen-Specific IgG+ Memory B Cells.A cell electrofusion microfluidic device integrated with 3D thin-film microelectrode arrays.Cross-Reactive and Potent Neutralizing Antibody Responses in Human Survivors of Natural Ebolavirus Infection.Unusual features of vaccinia virus extracellular virion form neutralization resistance revealed in human antibody responses to the smallpox vaccine.Optically-Induced Cell Fusion on Cell Pairing Microstructures.A microfluidic approach towards hybridoma generation for cancer immunotherapyStructural basis for nonneutralizing antibody competition at antigenic site II of the respiratory syncytial virus fusion protein.The rapid generation of recombinant functional monoclonal antibodies from individual, antigen-specific bone marrow-derived plasma cells isolated using a novel fluorescence-based method.Accessing the human repertoire for broadly neutralizing HIV antibodies.From rabbit antibody repertoires to rabbit monoclonal antibodiesDroplet microfluidics for high-throughput biological assays.Monoclonal antibodies based on hybridoma technology.Rare, high-affinity anti-pathogen antibodies from human repertoires, discovered using microfluidics and molecular genomics.Novel in vitro booster vaccination to rapidly generate antigen-specific human monoclonal antibodies.Monoclonal Antibodies Against the Staphylococcus aureus Bicomponent Leukotoxin AB Isolated Following Invasive Human Infection Reveal Diverse Binding and Modes of Action.The systematic study of the electroporation and electrofusion of B16-F1 and CHO cells in isotonic and hypotonic buffer.Dielectrophoresis-assisted massively parallel cell pairing and fusion based on field constriction created by a micro-orifice array sheet.Recent advances in the study of human antibody responses to influenza virus using optimized human hybridoma approaches.Cell counting tool parameters optimization approach for electroporation efficiency determination of attached cells in phase contrast images.Principles of Broad and Potent Antiviral Human Antibodies: Insights for Vaccine Design.Neutralizing human antibodies prevent Zika virus replication and fetal disease in mice.Persistence of circulating memory B cell clones with potential for dengue virus disease enhancement for decades following infection.Cross-Neutralizing and Protective Human Antibody Specificities to Poxvirus InfectionsEmerging studies of human HIV-specific antibody repertoires.
P2860
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P2860
An optimized electrofusion-based protocol for generating virus-specific human monoclonal antibodies
description
2008 nî lūn-bûn
@nan
2008年の論文
@ja
2008年学术文章
@wuu
2008年学术文章
@zh-cn
2008年学术文章
@zh-hans
2008年学术文章
@zh-my
2008年学术文章
@zh-sg
2008年學術文章
@yue
2008年學術文章
@zh
2008年學術文章
@zh-hant
name
An optimized electrofusion-bas ...... ic human monoclonal antibodies
@en
An optimized electrofusion-bas ...... c human monoclonal antibodies.
@nl
type
label
An optimized electrofusion-bas ...... ic human monoclonal antibodies
@en
An optimized electrofusion-bas ...... c human monoclonal antibodies.
@nl
prefLabel
An optimized electrofusion-bas ...... ic human monoclonal antibodies
@en
An optimized electrofusion-bas ...... c human monoclonal antibodies.
@nl
P2093
P2860
P1476
An optimized electrofusion-bas ...... ic human monoclonal antibodies
@en
P2093
Frances S House
Patricia A McGraw
Xiaocong Yu
P2860
P304
P356
10.1016/J.JIM.2008.04.008
P577
2008-05-05T00:00:00Z