Binding energy and catalysis. Fluorinated and deoxygenated glycosides as mechanistic probes of Escherichia coli (lacZ) beta-galactosidase.
about
Fundamental challenges in mechanistic enzymology: progress toward understanding the rate enhancements of enzymesCrystal structure of a 117 kDa glucansucrase fragment provides insight into evolution and product specificity of GH70 enzymesBiochemical and structural assessment of the 1-N-azasugar GalNAc-isofagomine as a potent family 20 beta-N-acetylhexosaminidase inhibitorPractical considerations when using temperature to obtain rate constants and activation thermodynamics of enzymes with two catalytic steps: native and N460T-beta-galactosidase (E. coli) as examplesCrystal structure of an inverting GH 43 1,5-alpha-L-arabinanase from Geobacillus stearothermophilus complexed with its substrateN-Acetyl glycals are tight-binding and environmentally insensitive inhibitors of hexosaminidasesCrystallographic observation of a covalent catalytic intermediate in a beta-glycosidaseInsights into transition state stabilization of the beta-1,4-glycosidase Cex by covalent intermediate accumulation in active site mutantsLong-lived glycosyl-enzyme intermediate mimic produced by formate re-activation of a mutant endoglucanase lacking its catalytic nucleophileSynthesis and characterization of novel lacZ gene reporter molecules: detection of beta-galactosidase activity by 19F nuclear magnetic resonance of polyglycosylated fluorinated vitamin B6Directed evolution of a glycosynthase from Agrobacterium sp. increases its catalytic activity dramatically and expands its substrate repertoire.LacZ β-galactosidase: structure and function of an enzyme of historical and molecular biological importance.Approaches to labeling and identification of active site residues in glycosidases.Identification of the active-site nucleophile in 6-phospho-beta-galactosidase from Staphylococcus aureus by labelling with synthetic inhibitors.Enzymatic circularization of a malto-octaose linear chain studied by stochastic reaction path calculations on cyclodextrin glycosyltransferase.Catalytic mechanism and specificity for hydrolysis and transglycosylation reactions of cytosolic beta-glucosidase from guinea pig liver.The beta-galactosidase (Escherichia coli) reaction is partly facilitated by interactions of His-540 with the C6 hydroxyl of galactose.Mechanistic investigations of unsaturated glucuronyl hydrolase from Clostridium perfringens.Crystal structure of 4-nitro-phenyl 6-O-ethyl-β-d-galacto-pyran-oside monohydrate.Larger increases in sensitivity to paracatalytic inactivation than in catalytic competence during experimental evolution of the second beta-galactosidase of Escherichia coli.Role of non-covalent enzyme-substrate interactions in the reaction catalysed by cellobiose phosphorylase from Cellulomonas uda.Identification of Glu-519 as the catalytic nucleophile in beta-mannosidase 2A from Cellulomonas fimi.A study of the relationships of interactions between Asp-201, Na+ or K+, and galactosyl C6 hydroxyl and their effects on binding and reactivity of beta-galactosidase.Biological evaluation of a series of 2-acetamido-2-deoxy-D-glucose analogs towards cellular glycosaminoglycan and protein synthesis in vitro.A Chiron approach towards the stereoselective synthesis of polyfluorinated carbohydrates
P2860
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P2860
Binding energy and catalysis. Fluorinated and deoxygenated glycosides as mechanistic probes of Escherichia coli (lacZ) beta-galactosidase.
description
1992 nî lūn-bûn
@nan
1992年の論文
@ja
1992年論文
@yue
1992年論文
@zh-hant
1992年論文
@zh-hk
1992年論文
@zh-mo
1992年論文
@zh-tw
1992年论文
@wuu
1992年论文
@zh
1992年论文
@zh-cn
name
Binding energy and catalysis. ...... oli (lacZ) beta-galactosidase.
@en
type
label
Binding energy and catalysis. ...... oli (lacZ) beta-galactosidase.
@en
prefLabel
Binding energy and catalysis. ...... oli (lacZ) beta-galactosidase.
@en
P2093
P2860
P356
P1433
P1476
Binding energy and catalysis. ...... oli (lacZ) beta-galactosidase.
@en
P2093
P2860
P304
P356
10.1042/BJ2860721
P407
P478
286 ( Pt 3)
P577
1992-09-01T00:00:00Z