Functional expression of gastric H,K-ATPase using the baculovirus expression system. - Wikidata - wikimedia - TriplyDB

Functional expression of gastric H,K-ATPase using the baculovirus expression system.

Functional expression of gastric H,K-ATPase using the baculovirus expression system.

http://www.wikidata.org/entity/Q42069681

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The non-gastric H,K-ATPase is oligomycin-sensitive and can function as an H+,NH4(+)-ATPase H,K-ATPase alpha subunit C-terminal membrane topology: epitope tags in the insect cell expression system.The alpha and beta subunits of the Na,K-ATPase can assemble at the plasma membrane into functional enzyme Role of negatively charged residues in the fifth and sixth transmembrane domains of the catalytic subunit of gastric H+,K+-ATPase.Overexpression of integral membrane proteins for structural studies.Mutational study on the roles of disulfide bonds in the beta-subunit of gastric H+,K+-ATPase.Involvement of glutamic acid 820 in K+ and SCH 28080 binding to gastric H+,K(+)-ATPase.A cytoplasmic region of the Na,K-ATPase alpha-subunit is necessary for specific alpha/alpha association.Ca2+ signaling in Sf9 insect cells and the functional expression of a rat brain M5 muscarinic receptor.The negative charge of glutamic acid-820 in the gastric H+,K+-ATPase alpha-subunit is essential for K+ activation of the enzyme activity.The beta-subunits of Na+,K+-ATPase and gastric H+,K+-ATPase have a high preference for their own alpha-subunit and affect the K+ affinity of these enzymes.Glycosylation is essential for biosynthesis of functional gastric H+,K+-ATPase in insect cells.Co-localization and functional coupling of creatine kinase B and gastric H+/K(+)-ATPase on the apical membrane and the tubulovesicular system of parietal cells.Constitutive activation of gastric H+,K+-ATPase by a single mutation Chimeras of X+, K+-ATPases. The M1-M6 region of Na+, K+-ATPase is required for Na+-activated ATPase activity, whereas the M7-M10 region of H+, K+-ATPase is involved in K+ de-occlusion.K(+)-independent gastric H(+),K(+)-atpase activity. Dissociation of K(+)-independent dephosphorylation and preference for the E1 conformation by combined mutagenesis of transmembrane glutamate residues.The rat distal colon P-ATPase alpha subunit encodes a ouabain-sensitive H+, K+-ATPase.Conversion of the low affinity ouabain-binding site of non-gastric H,K-ATPase into a high affinity binding site by substitution of only five amino acids.

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P2860

Functional expression of gastric H,K-ATPase using the baculovirus expression system.

http://www.wikidata.org/entity/Q42069681

description

1993 nî lūn-bûn

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1993年の論文

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1993年論文

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1993年論文

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1993年論文

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1993年論文

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1993年論文

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1993年论文

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1993年论文

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1993年论文

@zh-cn

name

Functional expression of gastric H,K-ATPase using the baculovirus expression system.

@en

Functional expression of gastric H,K-ATPase using the baculovirus expression system.

@nl

type

label

Functional expression of gastric H,K-ATPase using the baculovirus expression system.

@en

Functional expression of gastric H,K-ATPase using the baculovirus expression system.

@nl

prefLabel

Functional expression of gastric H,K-ATPase using the baculovirus expression system.

@en

Functional expression of gastric H,K-ATPase using the baculovirus expression system.

@nl

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0014-5793(93)80237-O

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P1476

Functional expression of gastric H,K-ATPase using the baculovirus expression system.

@en

P2093

De Caluwé GL

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De Moel MP

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De Pont JJ

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Klaassen CH

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Swarts HG

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Van Uem TJ

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P2860

Characterization of a beta subunit of the gastric H+/K(+)-transporting ATPase

Rapid and efficient site-specific mutagenesis without phenotypic selection

Functional expression of the alpha 2 and alpha 3 isoforms of the Na,K-ATPase in baculovirus-infected insect cells.

An ion-transporting ATPase encodes multiple apical localization signals.

Functional analysis of the human alpha 2C-C4 adrenergic receptor in insect cells expressed by a luciferase-based baculovirus vector.

Development of baculovirus triple and quadruple expression vectors: co-expression of three or four bluetongue virus proteins and the synthesis of bluetongue virus-like particles in insect cells.

Baculovirus-mediated expression of the Na+/glucose cotransporter in Sf9 cells.

Functional expression of the human growth factor activatable Na+/H+ antiporter (NHE-1) in baculovirus-infected cells.

In vitro expression of bovine opsin using recombinant baculovirus: the role of glutamic acid (134) in opsin biosynthesis and glycosylation.

A baculovirus dual expression vector derived from the Autographa californica nuclear polyhedrosis virus polyhedrin and p10 promoters: co-expression of two influenza virus genes in insect cells.

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277-282

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scholarly article

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10.1016/0014-5793(93)80237-O

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3

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329

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1993-08-01T00:00:00Z

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8396043

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