Directed evolution of thymidine kinase for AZT phosphorylation using DNA family shuffling.
about
Diversification of catalytic function in a synthetic family of chimeric cytochrome p450sProdrugs for Gene-Directed Enzyme-Prodrug Therapy (Suicide Gene Therapy)Nucleoside binding site of herpes simplex type 1 thymidine kinase analyzed by X-ray crystallographyThe effect of substrate binding on the conformation and structural stability of Herpes simplex virus type 1 thymidine kinaseImproving nucleoside diphosphate kinase for antiviral nucleotide analogs activationBiochemical and structural characterization of (South)-methanocarbathymidine that specifically inhibits growth of herpes simplex virus type 1 thymidine kinase-transduced osteosarcoma cellsRetrovolution: HIV-driven evolution of cellular genes and improvement of anticancer drug activationGauging triple stores with actual biological data.Advances in directed molecular evolution of reporter genes.DNA shuffling method for generating highly recombined genes and evolved enzymes.Quantitative analysis of the effect of the mutation frequency on the affinity maturation of single chain Fv antibodies.Enhanced crossover SCRATCHY: construction and high-throughput screening of a combinatorial library containing multiple non-homologous crossovers.Thermostable mutants of the photoprotein aequorin obtained by in vitro evolution.Non-homologous recombination of deoxyribonucleoside kinases from human and Drosophila melanogaster yields human-like enzymes with novel activities.Revealing biases inherent in recombination protocols.In vitro and in vivo gene therapy vector evolution via multispecies interbreeding and retargeting of adeno-associated viruses.Prospecting metagenomic enzyme subfamily genes for DNA family shuffling by a novel PCR-based approachEngineering Kinases to Phosphorylate Nucleoside Analogs for Antiviral and Cancer Therapy.Importance of compartment formation for a self-encoding systemAlgorithms for optimizing cross-overs in DNA shuffling.Discovery of superior enzymes by directed molecular evolution.Assessing directed evolution methods for the generation of biosynthetic enzymes with potential in drug biosynthesis.Directed evolution of an orthogonal nucleoside analog kinase via fluorescence-activated cell sorting.A few amino acid substitutions can convert deoxyribonucleoside kinase specificity from pyrimidines to purines.Direct positive selection for improved nitroreductase variants using SOS triggering of bacteriophage lambda lytic cycle.Limited mutagenesis increases the stability of human carboxypeptidase U (TAFIa) and demonstrates the importance of CPU stability over proCPU concentration in down-regulating fibrinolysis.Monitoring adenoviral DNA delivery, using a mutant herpes simplex virus type 1 thymidine kinase gene as a PET reporter gene.Retroviral transduction of cancer cell lines with the gene encoding Drosophila melanogaster multisubstrate deoxyribonucleoside kinase.The rational of catalytic activity of herpes simplex virus thymidine kinase. a combined biochemical and quantum chemical study.Active site mutants of Drosophila melanogaster multisubstrate deoxyribonucleoside kinase.Substrate diversity of herpes simplex virus thymidine kinase. Impact Of the kinematics of the enzyme.Cloning and characterization of the multisubstrate deoxyribonucleoside kinase of Drosophila melanogaster.Colorless green ideas..
P2860
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P2860
Directed evolution of thymidine kinase for AZT phosphorylation using DNA family shuffling.
description
1999 nî lūn-bûn
@nan
1999年の論文
@ja
1999年学术文章
@wuu
1999年学术文章
@zh
1999年学术文章
@zh-cn
1999年学术文章
@zh-hans
1999年学术文章
@zh-my
1999年学术文章
@zh-sg
1999年學術文章
@yue
1999年學術文章
@zh-hant
name
Directed evolution of thymidin ...... on using DNA family shuffling.
@en
Directed evolution of thymidin ...... on using DNA family shuffling.
@nl
type
label
Directed evolution of thymidin ...... on using DNA family shuffling.
@en
Directed evolution of thymidin ...... on using DNA family shuffling.
@nl
prefLabel
Directed evolution of thymidin ...... on using DNA family shuffling.
@en
Directed evolution of thymidin ...... on using DNA family shuffling.
@nl
P2093
P2860
P356
P1433
P1476
Directed evolution of thymidin ...... on using DNA family shuffling.
@en
P2093
Christians FC
Scapozza L
Stemmer WP
P2860
P2888
P304
P356
10.1038/7003
P577
1999-03-01T00:00:00Z
P6179
1042088583