Cloning structural gene sacB, which codes for exoenzyme levansucrase of Bacillus subtilis: expression of the gene in Escherichia coli
about
Homing endonuclease mediated gene targeting in Anopheles gambiae cells and embryosStrategies used for genetically modifying bacterial genome: site-directed mutagenesis, gene inactivation, and gene over-expressionA positive selection vector for cloning high molecular weight DNA by the bacteriophage P1 system: improved cloning efficacy.Modification of the Creator recombination system for proteomics applications--improved expression by addition of splice sites.Impact of the rpoS genotype for acid resistance patterns of pathogenic and probiotic Escherichia coli.Development of a broad-host-range sacB-based vector for unmarked allelic exchangeConstruction and characterization of an isogenic slt-ii deletion mutant of enterohemorrhagic Escherichia coli.The major immediate-early gene ie3 of mouse cytomegalovirus is essential for viral growth.Identification of the nik gene cluster of Brucella suis: regulation and contribution to urease activity.Positive and negative selection using the tetA-sacB cassette: recombineering and P1 transduction in Escherichia coli.A novel three-protein two-component system provides a regulatory twist on an established circuit to modulate expression of the cbbI region of Rhodopseudomonas palustris CGA010The synchrony phenotype persists after elimination of multiple GATC sites from the dnaA promoter of Escherichia coli.Functional characterization of Kaposi's sarcoma-associated herpesvirus open reading frame K8 by bacterial artificial chromosome-based mutagenesis.Development of genetically stable Escherichia coli strains for poly(3-hydroxypropionate) productionFunctional characterization of Kaposi's sarcoma-associated herpesvirus ORF45 by bacterial artificial chromosome-based mutagenesis.MudSacI, a transposon with strong selectable and counterselectable markers: use for rapid mapping of chromosomal mutations in Salmonella typhimurium.An Integrated System for Precise Genome Modification in Escherichia coliA set of powerful negative selection systems for unmodified Enterobacteriaceae.PCR-Based Seamless Genome Editing with High Efficiency and Fidelity in Escherichia coli.Characterization of IS476 and its role in bacterial spot disease of tomato and pepperIdentification of the minimal region in lipase ABC transporter recognition domain of Pseudomonas fluorescens for secretion and fluorescence of green fluorescent proteinExpression and regulation of a Vibrio alginolyticus sucrose utilization system cloned in Escherichia coliPositive selection procedure for entrapment of insertion sequence elements in gram-negative bacteria.5'-noncoding region sacR is the target of all identified regulation affecting the levansucrase gene in Bacillus subtilisMajor histocompatibility complex class I allele-specific cooperative and competitive interactions between immune evasion proteins of cytomegalovirus.Isolation of DNA encoding sucrase genes from Streptococcus salivarius and partial characterization of the enzymes expressed in Escherichia coli.Direct selection of cloned DNA in Bacillus subtilis based on sucrose-induced lethalityConstruction of new cloning, lacZ reporter and scarless-markerless suicide vectors for genetic studies in Aggregatibacter actinomycetemcomitans.High-Efficiency, Two-Step Scarless-Markerless Genome Genetic Modification in Salmonella enterica.Development of a markerless genetic exchange method for Methanosarcina acetivorans C2A and its use in construction of new genetic tools for methanogenic archaea.whmD is an essential mycobacterial gene required for proper septation and cell division.An improved method to knock out the asd gene of Salmonella enterica serovar PullorumFructan and its relationship to abiotic stress tolerance in plants.Development of a markerless genetic exchange system for Desulfovibrio vulgaris Hildenborough and its use in generating a strain with increased transformation efficiency.The Agrobacterium tumefaciens virB4 gene product is an essential virulence protein requiring an intact nucleoside triphosphate-binding domain.Large scale validation of an efficient CRISPR/Cas-based multi gene editing protocol in Escherichia coli.Improved bacterial mutagenesis by high-frequency allele exchange, demonstrated in Clostridium difficile and Streptococcus suis.The Brucella suis homologue of the Agrobacterium tumefaciens chromosomal virulence operon chvE is essential for sugar utilization but not for survival in macrophages.Characterization and mutational analysis of three allelic lsc genes encoding levansucrase in Pseudomonas syringae.Counterselectable markers: untapped tools for bacterial genetics and pathogenesis.
P2860
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P2860
Cloning structural gene sacB, which codes for exoenzyme levansucrase of Bacillus subtilis: expression of the gene in Escherichia coli
description
1983 nî lūn-bûn
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name
Cloning structural gene sacB, ...... f the gene in Escherichia coli
@ast
Cloning structural gene sacB, ...... f the gene in Escherichia coli
@en
type
label
Cloning structural gene sacB, ...... f the gene in Escherichia coli
@ast
Cloning structural gene sacB, ...... f the gene in Escherichia coli
@en
prefLabel
Cloning structural gene sacB, ...... f the gene in Escherichia coli
@ast
Cloning structural gene sacB, ...... f the gene in Escherichia coli
@en
P2093
P2860
P1476
Cloning structural gene sacB, ...... f the gene in Escherichia coli
@en
P2093
P2860
P304
P407
P577
1983-03-01T00:00:00Z