An exciting but challenging road ahead for computational enzyme design
about
Fundamental challenges in mechanistic enzymology: progress toward understanding the rate enhancements of enzymesWhy reinvent the wheel? Building new proteins based on ready-made partsExpanding proteostasis by membrane trafficking networksRole of substrate dynamics in protein prenylation reactionsOptimization of the In-Silico-Designed Kemp Eliminase KE70 by Computational Design and Directed EvolutionEngineering a model protein cavity to catalyze the Kemp eliminationComputation-guided backbone grafting of a discontinuous motif onto a protein scaffoldJanus: Prediction and Ranking of Mutations Required for Functional Interconversion of EnzymesComputational redesign of a mononuclear zinc metalloenzyme for organophosphate hydrolysisDesigning Allosteric Control into Enzymes by Chemical Rescue of StructureComputational design of ligand-binding proteins with high affinity and selectivityRational Design of a Colorimetric pH Sensor from a Soluble Retinoic Acid ChaperoneBridging the gaps in design methodologies by evolutionary optimization of the stability and proficiency of designed Kemp eliminase KE59Dynamics and hydration explain failed functional transformation in dehalogenase designThe use of ene adducts to study and engineer enoyl-thioester reductasesInstalling hydrolytic activity into a completely de novo protein frameworkCoupling Protein Side-Chain and Backbone Flexibility Improves the Re-design of Protein-Ligand SpecificityProtein folding and de novo protein design for biotechnological applicationsComputational protein engineering: bridging the gap between rational design and laboratory evolution.Creating novel protein scripts beyond natural alphabetsElementary tetrahelical protein design for diverse oxidoreductase functions.Binding pocket optimization by computational protein design.Optimizing non-natural protein function with directed evolution.Restricted sidechain plasticity in the structures of native proteins and complexes.Challenges and advances in validating enzyme design proposals: the case of kemp eliminase catalysisHighly diverse protein library based on the ubiquitous (β/α)₈ enzyme fold yields well-structured proteins through in vitro folding selection.Applying physics-based scoring to calculate free energies of binding for single amino acid mutations in protein-protein complexesIsotope effects as probes for enzyme catalyzed hydrogen-transfer reactions.Directed Evolution: Past, Present and Future.Expanding the enzyme universe: accessing non-natural reactions by mechanism-guided directed evolution.Extensive site-directed mutagenesis reveals interconnected functional units in the alkaline phosphatase active site.Dissecting enzyme function with microfluidic-based deep mutational scanningComputational strategies for the design of new enzymatic functionsUse of an Improved Matching Algorithm to Select Scaffolds for Enzyme Design Based on a Complex Active Site ModelConservation of flexible residue clusters among structural and functional enzyme homologuesNavigating the protein fitness landscape with Gaussian processesKinetic isotope effects as a probe of hydrogen transfers to and from common enzymatic cofactorsEnzyme recruitment and its role in metabolic expansion.A highly efficient cocaine-detoxifying enzyme obtained by computational design.Lessons about protein stability from in vitro selections.
P2860
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P2860
An exciting but challenging road ahead for computational enzyme design
description
2010 nî lūn-bûn
@nan
2010 թուականի Հոկտեմբերին հրատարակուած գիտական յօդուած
@hyw
2010 թվականի հոտեմբերին հրատարակված գիտական հոդված
@hy
2010年の論文
@ja
2010年論文
@yue
2010年論文
@zh-hant
2010年論文
@zh-hk
2010年論文
@zh-mo
2010年論文
@zh-tw
2010年论文
@wuu
name
An exciting but challenging road ahead for computational enzyme design
@ast
An exciting but challenging road ahead for computational enzyme design
@en
An exciting but challenging road ahead for computational enzyme design
@nl
type
label
An exciting but challenging road ahead for computational enzyme design
@ast
An exciting but challenging road ahead for computational enzyme design
@en
An exciting but challenging road ahead for computational enzyme design
@nl
prefLabel
An exciting but challenging road ahead for computational enzyme design
@ast
An exciting but challenging road ahead for computational enzyme design
@en
An exciting but challenging road ahead for computational enzyme design
@nl
P2860
P3181
P356
P1433
P1476
An exciting but challenging road ahead for computational enzyme design
@en
P2860
P304
P3181
P356
10.1002/PRO.481
P407
P50
P577
2010-10-01T00:00:00Z