Employing Escherichia coli to functionally express, purify, and characterize a human transporter
about
Heterologous expression and purification systems for structural proteomics of mammalian membrane proteinsStrategies of bacterial over expression of membrane transporters relevant in human health: the successful case of the three members of OCTN subfamilyIncreased sulfate uptake by E. coli overexpressing the SLC26-related SulP protein Rv1739c from Mycobacterium tuberculosisA mechanism for intracellular release of Na+ by neurotransmitter/sodium symporters.Engineering membrane protein overproduction in Escherichia coli.Substrate specificity of the Escherichia coli outer membrane protease OmpT.Tuning microbial hosts for membrane protein production.Substrate-dependent proton antiport in neurotransmitter:sodium symporters.Frog oocytes to unveil the structure and supramolecular organization of human transport proteins.Water pumps.Crystal structures of fusion proteins with large-affinity tags.Monitoring the function of membrane transport proteins in detergent-solubilized form.Experimental conditions can obscure the second high-affinity site in LeuT.Interaction of α-Lipoic Acid with the Human Na+/Multivitamin Transporter (hSMVT).A Versatile Strategy for Production of Membrane Proteins with Diverse Topologies: Application to Investigation of Bacterial Homologues of Human Divalent Metal Ion and Nucleoside Transporters.Tricks of the trade used to accelerate high-resolution structure determination of membrane proteins.OCTN cation transporters in health and disease: role as drug targets and assay development.The sodium/multivitamin transporter: a multipotent system with therapeutic implications.Current strategies for protein production and purification enabling membrane protein structural biology.Bridging the gap: a GFP-based strategy for overexpression and purification of membrane proteins with intra and extracellular C-termini.Combinatorial method for overexpression of membrane proteins in Escherichia coli.State-dependent conformations of the translocation pathway in the tyrosine transporter Tyt1, a novel neurotransmitter:sodium symporter from Fusobacterium nucleatum.Proteoliposomes as tool for assaying membrane transporter functions and interactions with xenobiotics.High-efficiency production of bioactive recombinant human fibroblast growth factor 18 in Escherichia coli and its effects on hair follicle growth.Reconstitution in liposomes of the functionally active human OCTN1 (SLC22A4) transporter overexpressed in Escherichia coli.Analysis of recombinant tagged equilibrative nucleoside transporter 1 (ENT1) expressed in E. coli.
P2860
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P2860
Employing Escherichia coli to functionally express, purify, and characterize a human transporter
description
2002 nî lūn-bûn
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2002 թուականի Յունիսին հրատարակուած գիտական յօդուած
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2002 թվականի հունիսին հրատարակված գիտական հոդված
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2002年の論文
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2002年学术文章
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2002年学术文章
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name
Employing Escherichia coli to ...... aracterize a human transporter
@ast
Employing Escherichia coli to ...... aracterize a human transporter
@en
Employing Escherichia coli to ...... aracterize a human transporter
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type
label
Employing Escherichia coli to ...... aracterize a human transporter
@ast
Employing Escherichia coli to ...... aracterize a human transporter
@en
Employing Escherichia coli to ...... aracterize a human transporter
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prefLabel
Employing Escherichia coli to ...... aracterize a human transporter
@ast
Employing Escherichia coli to ...... aracterize a human transporter
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Employing Escherichia coli to ...... aracterize a human transporter
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P2860
P356
P1476
Employing Escherichia coli to ...... aracterize a human transporter
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P2093
Ernest M Wright
Matthias Quick
P2860
P304
P356
10.1073/PNAS.132266599
P407
P577
2002-06-19T00:00:00Z