PFunkel: efficient, expansive, user-defined mutagenesis.
about
The genetic code constrains yet facilitates Darwinian evolutionElucidating the molecular architecture of adaptation via evolve and resequence experimentsEngineering of Kuma030: A Gliadin Peptidase That Rapidly Degrades Immunogenic Gliadin Peptides in Gastric Conditions.Environmental changes bridge evolutionary valleysTolerance of a Knotted Near-Infrared Fluorescent Protein to Random Circular PermutationDetermination of binding affinity upon mutation for type I dockerin-cohesin complexes from Clostridium thermocellum and Clostridium cellulolyticum using deep sequencingRapid fine conformational epitope mapping using comprehensive mutagenesis and deep sequencing.Software for the analysis and visualization of deep mutational scanning data.A comprehensive, high-resolution map of a gene's fitness landscapeSingle-mutation fitness landscapes for an enzyme on multiple substrates reveal specificity is globally encodedAn experimentally determined evolutionary model dramatically improves phylogenetic fit.The inherent mutational tolerance and antigenic evolvability of influenza hemagglutinin.Engineering an improved light-induced dimer (iLID) for controlling the localization and activity of signaling proteins.Massively parallel single-amino-acid mutagenesisDirected evolution of improved zinc finger methyltransferases.Lessons from diversity of directed evolution experiments by an analysis of 3,000 mutations.Improvement of biocatalysts for industrial and environmental purposes by saturation mutagenesisMeasuring the activity of protein variants on a large scale using deep mutational scanning.High-resolution sequence-function mapping of full-length proteins.De novo assembly and next-generation sequencing to analyse full-length gene variants from codon-barcoded libraries.The Structure of a Thermophilic Kinase Shapes Fitness upon Random Circular PermutationPlasmid-based one-pot saturation mutagenesis.A Simple Combinatorial Codon Mutagenesis Method for Targeted Protein EngineeringAmino acid substitutions in random mutagenesis libraries: lessons from analyzing 3000 mutations.Primerize-2D: automated primer design for RNA multidimensional chemical mapping.Complete mapping of viral escape from neutralizing antibodiesSite-directed mutant libraries for isolating minimal mutations yielding functional changes.Directed evolution 2.0: improving and deciphering enzyme properties.Rapid and Programmable Protein Mutagenesis Using Plasmid Recombineering.The power of multiplexed functional analysis of genetic variants.Deep sequencing methods for protein engineering and design.Modern methods for laboratory diversification of biomolecules.Targeted mutagenesis: A sniper-like diversity generator in microbial engineering.Base-resolution stratification of cancer mutations using functional variomics.Darwin Assembly: fast, efficient, multi-site bespoke mutagenesis.Simple-MSSM: a simple and efficient method for simultaneous multi-site saturation mutagenesis.Multiplexed assays of variant effects contribute to a growing genotype-phenotype atlas
P2860
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P2860
PFunkel: efficient, expansive, user-defined mutagenesis.
description
2012 nî lūn-bûn
@nan
2012 թուականի Դեկտեմբերին հրատարակուած գիտական յօդուած
@hyw
2012 թվականի դեկտեմբերին հրատարակված գիտական հոդված
@hy
2012年の論文
@ja
2012年論文
@yue
2012年論文
@zh-hant
2012年論文
@zh-hk
2012年論文
@zh-mo
2012年論文
@zh-tw
2012年论文
@wuu
name
PFunkel: efficient, expansive, user-defined mutagenesis.
@ast
PFunkel: efficient, expansive, user-defined mutagenesis.
@en
PFunkel: efficient, expansive, user-defined mutagenesis.
@nl
type
label
PFunkel: efficient, expansive, user-defined mutagenesis.
@ast
PFunkel: efficient, expansive, user-defined mutagenesis.
@en
PFunkel: efficient, expansive, user-defined mutagenesis.
@nl
prefLabel
PFunkel: efficient, expansive, user-defined mutagenesis.
@ast
PFunkel: efficient, expansive, user-defined mutagenesis.
@en
PFunkel: efficient, expansive, user-defined mutagenesis.
@nl
P2860
P1433
P1476
PFunkel: efficient, expansive, user-defined mutagenesis
@en
P2093
Elad Firnberg
P2860
P304
P356
10.1371/JOURNAL.PONE.0052031
P407
P577
2012-12-17T00:00:00Z