Isolation of a thermostable enzyme variant by cloning and selection in a thermophile.
about
Extremely thermophilic microorganisms as metabolic engineering platforms for production of fuels and industrial chemicalsCooling and slowing of trapped antiprotons below 100 meVParallel genotypic adaptation: when evolution repeats itselfIn vivo selection of better self-splicing introns in Escherichia coli: the role of the P1 extension helix of the Tetrahymena intron.Investigating and Engineering Enzymes by Genetic Selection.An autonomously replicating transforming vector for Sulfolobus solfataricus.Aspartate kinase-independent lysine synthesis in an extremely thermophilic bacterium, Thermus thermophilus: lysine is synthesized via alpha-aminoadipic acid not via diaminopimelic acid.Construction and transformation of a Thermotoga-E. coli shuttle vector.Cloning and characterization of tRNA (m1A58) methyltransferase (TrmI) from Thermus thermophilus HB27, a protein required for cell growth at extreme temperatures.Discovery of superior enzymes by directed molecular evolution.A ternary conjugation system for the construction of DNA libraries for Geobacillus kaustophilus HTA426.Some (bacilli) like it hot: genomics of Geobacillus species.Genetic toolbox for controlled expression of functional proteins in Geobacillus sppThymidine kinase mutants obtained by random sequence selection.Site-directed mutant libraries for isolating minimal mutations yielding functional changes.Tuning the activity of an enzyme for unusual environments: sequential random mutagenesis of subtilisin E for catalysis in dimethylformamide.Genetics and potential biotechnological applications of thermophilic and extremely thermophilic micro-organisms.Engineering multiple properties of a protein by combinatorial mutagenesis.The denaturation and degradation of stable enzymes at high temperatures.Functionality and the evolution of marginal stability in proteins: inferences from lattice simulationsSupra-operonic clusters of functionally related genes (SOCs) are a source of horizontal gene co-transfers.Novel thermostable antibiotic resistance enzymes from the Atlantis II Deep Red Sea brine pool.Exploring the active site of chorismate mutase by combinatorial mutagenesis and selection: the importance of electrostatic catalysis.Extremophiles in biofuel synthesis.Microbial proteases: detection, production, and genetic improvement.Development and application of a PCR-targeted gene disruption method for studying CelR function in Thermobifida fusca.Markerless Gene Deletion with Cytosine Deaminase in Thermus thermophilus Strain HB27.Mutational biases influence parallel adaptation.Rapid evolution of novel traits in microorganisms.Production of recombinant alpha-galactosidases in Thermus thermophilus.Experimental evolution of enzyme temperature activity profile: selection in vivo and characterization of low-temperature-adapted mutants of Pyrococcus furiosus ornithine carbamoyltransferase.Functional and evolutionary relationship between arginine biosynthesis and prokaryotic lysine biosynthesis through alpha-aminoadipate.Engineering of a cold-adapted protease by sequential random mutagenesis and a screening system.Thermoadaptation of alpha-galactosidase AgaB1 in Thermus thermophilus.Pyrimidine biosynthesis genes (pyrE and pyrF) of an extreme thermophile, Thermus thermophilus.Molecular cloning of the pyrE gene from the extreme thermophile Thermus flavusSpontaneous tandem sequence duplications reverse the thermal stability of carboxyl-terminal modified 3-isopropylmalate dehydrogenase.A new Thermus-Escherichia coli shuttle integration vector system.Molecular cloning and sequence analysis of the crtB gene of Thermus thermophilus HB27, an extreme thermophile producing carotenoid pigmentsExpression of the insecticidal protein gene from Bacillus thuringiensis subsp. aizawai in Bacillus subtilis and in the thermophile Bacillus stearothermophilus by using the alpha-amylase promoter of the thermophile.
P2860
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P2860
Isolation of a thermostable enzyme variant by cloning and selection in a thermophile.
description
1986 nî lūn-bûn
@nan
1986年の論文
@ja
1986年論文
@yue
1986年論文
@zh-hant
1986年論文
@zh-hk
1986年論文
@zh-mo
1986年論文
@zh-tw
1986年论文
@wuu
1986年论文
@zh
1986年论文
@zh-cn
name
Isolation of a thermostable enzyme variant by cloning and selection in a thermophile.
@ast
Isolation of a thermostable enzyme variant by cloning and selection in a thermophile.
@en
type
label
Isolation of a thermostable enzyme variant by cloning and selection in a thermophile.
@ast
Isolation of a thermostable enzyme variant by cloning and selection in a thermophile.
@en
prefLabel
Isolation of a thermostable enzyme variant by cloning and selection in a thermophile.
@ast
Isolation of a thermostable enzyme variant by cloning and selection in a thermophile.
@en
P2093
P2860
P356
P1476
Isolation of a thermostable enzyme variant by cloning and selection in a thermophile.
@en
P2093
P2860
P304
P356
10.1073/PNAS.83.3.576
P407
P577
1986-02-01T00:00:00Z