Site-directed mutagenesis of beta-lactamase I. Single and double mutants of Glu-166 and Lys-73.
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Three decades of beta-lactamase inhibitorsThe Acylation Mechanism of CTX-M β-Lactamase at 0.88 Å ResolutionThe active site protonation states of perdeuterated Toho-1 β-lactamase determined by neutron diffraction support a role for Glu166 as the general base in acylationNeutron and X-ray Crystal Structures of a Perdeuterated Enzyme Inhibitor Complex Reveal the Catalytic Proton Network of the Toho-1 -Lactamase for the Acylation ReactionStructural insights into the broadened substrate profile of the extended-spectrum β-lactamase OXY-1-1 from Klebsiella oxytocaThe catalytic mechanism of beta-lactamases: NMR titration of an active-site lysine residue of the TEM-1 enzymeThe importance of a critical protonation state and the fate of the catalytic steps in class A beta-lactamases and penicillin-binding proteins.Roles of amino acids 161 to 179 in the PSE-4 omega loop in substrate specificity and in resistance to ceftazidimeCan molecular dynamics and QM/MM solve the penicillin binding protein protonation puzzle?Different intermediate populations formed by tazobactam, sulbactam, and clavulanate reacting with SHV-1 beta-lactamases: Raman crystallographic evidenceVariations within class-A β-lactamase physiochemical properties reflect evolutionary and environmental patterns, but not antibiotic specificityPrimary structure of OXA-3 and phylogeny of oxacillin-hydrolyzing class D beta-lactamasesExtended-spectrum and inhibitor-resistant TEM-type beta-lactamases: mutations, specificity, and three-dimensional structure.pKa calculations for class A beta-lactamases: influence of substrate binding.Molecular and biochemical characterization of the chromosome-encoded class A beta-lactamase BCL-1 from Bacillus clausii.Comparison of the sequences of class A beta-lactamases and of the secondary structure elements of penicillin-recognizing proteinsMutation of the active site carboxy-lysine (K70) of OXA-1 beta-lactamase results in a deacylation-deficient enzyme.Active-Site Protonation States in an Acyl-Enzyme Intermediate of a Class A β-Lactamase with a Monobactam SubstrateSite-directed mutagenesis of beta-lactamase I: role of Glu-166.A dramatic change in the rate-limiting step of beta-lactam hydrolysis results from the substitution of the active-site serine residue by a cysteine in the class-C beta-lactamase of Enterobacter cloacae 908RSite-directed mutagenesis of the Streptomyces R61 DD-peptidase. Catalytic function of the conserved residues around the active site and a comparison with class-A and class-C beta-lactamases.Electrophoretic assay for penicillinase: substrate specificity screening by parallel CE with an active pixel sensor.Site-directed mutagenesis of the mecA gene from a methicillin-resistant strain of Staphylococcus aureus.Beta-lactamases and bacterial resistance to antibiotics.Catalytic properties of class A beta-lactamases: efficiency and diversity.The Streptomyces-produced antibiotic fosfomycin is a promiscuous substrate for archaeal isopentenyl phosphate kinase.The other kind of biological NMR--studies of enzyme-substrate interactions.Effect of side-chain amide thionation on turnover of beta-lactam substrates by beta-lactamases. Further evidence on the question of side-chain hydrogen-bonding in catalysis.Mechanism of acyl transfer by the class A serine beta-lactamase of Streptomyces albus G.Streptomyces albus G serine beta-lactamase. Probing of the catalytic mechanism via molecular modelling of mutant enzymes.Site-directed mutagenesis and substrate-induced inactivation of beta-lactamase I.The role of lysine-67 in a class C beta-lactamase is mainly electrostatic.Site-directed mutagenesis of proposed active-site residues of penicillin-binding protein 5 from Escherichia coliThe importance of the negative charge of beta-lactam compounds in the interactions with active-site serine DD-peptidases and beta-lactamases.pKa, MM, and QM studies of mechanisms of beta-lactamases and penicillin-binding proteins: acylation step.Understanding the acylation mechanisms of active-site serine penicillin-recognizing proteins: a molecular dynamics simulation study.A QM/MM study on the enzymatic inactivation of cefotaxime.Molecular mechanisms of antibiotic resistance: QM/MM modelling of deacylation in a class A beta-lactamase.A Tyrosine Residue Along with a Glutamic Acid of the Omega-Like Loop Governs the Beta-Lactamase Activity of MSMEG_4455 in Mycobacterium smegmatis.Targeting functional motifs of a protein family.
P2860
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P2860
Site-directed mutagenesis of beta-lactamase I. Single and double mutants of Glu-166 and Lys-73.
description
1990 nî lūn-bûn
@nan
1990年の論文
@ja
1990年論文
@yue
1990年論文
@zh-hant
1990年論文
@zh-hk
1990年論文
@zh-mo
1990年論文
@zh-tw
1990年论文
@wuu
1990年论文
@zh
1990年论文
@zh-cn
name
Site-directed mutagenesis of b ...... mutants of Glu-166 and Lys-73.
@en
Site-directed mutagenesis of b ...... mutants of Glu-166 and Lys-73.
@nl
type
label
Site-directed mutagenesis of b ...... mutants of Glu-166 and Lys-73.
@en
Site-directed mutagenesis of b ...... mutants of Glu-166 and Lys-73.
@nl
prefLabel
Site-directed mutagenesis of b ...... mutants of Glu-166 and Lys-73.
@en
Site-directed mutagenesis of b ...... mutants of Glu-166 and Lys-73.
@nl
P2093
P2860
P356
P1433
P1476
Site-directed mutagenesis of b ...... mutants of Glu-166 and Lys-73.
@en
P2093
Christensen H
P2860
P304
P356
10.1042/BJ2720613
P407
P577
1990-12-01T00:00:00Z