A general protocol for the generation of Nanobodies for structural biology - Test2 - elhamkhani - TriplyDB

A general protocol for the generation of Nanobodies for structural biology

A general protocol for the generation of Nanobodies for structural biology

http://www.wikidata.org/entity/Q34979957

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Crystal structure of a SLC11 (NRAMP) transporter reveals the basis for transition-metal ion transport Nanobody-derived nanobiotechnology tool kits for diverse biomedical and biotechnology applications Structure and flexibility of the endosomal Vps34 complex reveals the basis of its function on membranes.Crystal structure of the proteasomal deubiquitylation module Rpn8-Rpn11 The Molecular Mechanism of Shiga Toxin Stx2e Neutralization by a Single-domain Antibody Targeting the Cell Receptor-binding Domain Structure of a prokaryotic fumarate transporter reveals the architecture of the SLC26 family Nanobodies and recombinant binders in cell biology VHH antibodies: emerging reagents for the analysis of environmental chemicals Functional and Biochemical Characterization of Alvinella pompejana Cys-Loop Receptor Homologues A nanobody:GFP bacterial platform that enables functional enzyme display and easy quantification of display capacity Inhibition of type VI secretion by an anti-TssM llama nanobody Biophysical characterization data of the artificial protein Octarellin V.1 and binding test with its X-ray helpers Navigating Membrane Protein Structure, Dynamics, and Energy Landscapes Using Spin Labeling and EPR Spectroscopy.Nanobodies as Probes for Protein Dynamics in Vitro and in Cells.The changing landscape of membrane protein structural biology through developments in electron microscopy.Crystal structure of the Bloom's syndrome helicase indicates a role for the HRDC domain in conformational changes Structural basis of inhibition of lipid-linked oligosaccharide flippase PglK by a conformational nanobody.Mini-G proteins: Novel tools for studying GPCRs in their active conformation.Nanobodies to Study G Protein-Coupled Receptor Structure and Function.Expression cloning of camelid nanobodies specific for Xenopus embryonic antigens Structural insights into µ-opioid receptor activation.Distinct conformations of GPCR-β-arrestin complexes mediate desensitization, signaling, and endocytosis Generation of a nanobody targeting the paraflagellar rod protein of trypanosomes Outward-facing conformers of LacY stabilized by nanobodies.The dynamic conformational landscape of gamma-secretase.In vitro nanobody discovery for integral membrane protein targets Nanobody mediated inhibition of attachment of F18 Fimbriae expressing Escherichia coli.Streamlined method for parallel identification of single domain antibodies to membrane receptors on whole cells.Single domain antibodies as a powerful tool for high quality surface plasmon resonance studies.Noninvasive imaging of immune responses.Peptides in headlock--a novel high-affinity and versatile peptide-binding nanobody for proteomics and microscopy.Generation and characterization of CD1d-specific single-domain antibodies with distinct functional features.Comparative Analysis of Immune Repertoires between Bactrian Camel's Conventional and Heavy-Chain Antibodies A Rational Engineering Strategy for Designing Protein A-Binding Camelid Single-Domain Antibodies Active-site MMP-selective antibody inhibitors discovered from convex paratope synthetic libraries.Transient conformers of LacY are trapped by nanobodies.Large Diversity of Functional Nanobodies from a Camelid Immune Library Revealed by an Alternative Analysis of Next-Generation Sequencing Data Differentially expressed proteins in glioblastoma multiforme identified with a nanobody-based anti-proteome approach and confirmed by OncoFinder as possible tumor-class predictive biomarker candidates.The Atomic Structure of the Phage Tuc2009 Baseplate Tripod Suggests that Host Recognition Involves Two Different Carbohydrate Binding Modules.Nanobodies: site-specific labeling for super-resolution imaging, rapid epitope-mapping and native protein complex isolation.

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A general protocol for the generation of Nanobodies for structural biology

http://www.wikidata.org/entity/Q34979957

description

2014 nî lūn-bûn

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2014 թուականի Փետրուարին հրատարակուած գիտական յօդուած

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2014 թվականի փետրվարին հրատարակված գիտական հոդված

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2014年の論文

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2014年論文

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2014年論文

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2014年論文

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2014年論文

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2014年論文

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2014年论文

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name

A general protocol for the generation of Nanobodies for structural biology

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A general protocol for the generation of Nanobodies for structural biology

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A general protocol for the generation of Nanobodies for structural biology

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type

label

A general protocol for the generation of Nanobodies for structural biology

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A general protocol for the generation of Nanobodies for structural biology

@en

A general protocol for the generation of Nanobodies for structural biology

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prefLabel

A general protocol for the generation of Nanobodies for structural biology

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A general protocol for the generation of Nanobodies for structural biology

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A general protocol for the generation of Nanobodies for structural biology

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Nature Protocols

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A general protocol for the generation of Nanobodies for structural biology

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Armin Ruf

http://www.w3.org/2001/XMLSchema#string

Brian K Kobilka

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Sarah Triest

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Toon Laeremans

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Wim G J Hol

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P2860

Structure of a nanobody-stabilized active state of the β(2) adrenoceptor

Crystal structure of the β2 adrenergic receptor-Gs protein complex

Protein production and purification

A single-domain antibody fragment in complex with RNase A: non-canonical loop structures and nanomolar affinity using two CDR loops

Three camelid VHH domains in complex with porcine pancreatic alpha-amylase. Inhibition and versatility of binding topology

Crystal structure of the intrinsically flexible addiction antidote MazE

A camelid antibody fragment inhibits the formation of amyloid fibrils by human lysozyme

Toward chaperone-assisted crystallography: Protein engineering enhancement of crystal packing and X-ray phasing capabilities of a camelid single-domain antibody (VHH) scaffold

Nanobody-aided structure determination of the EpsI:EpsJ pseudopilin heterodimer from Vibrio vulnificus

Crystal Structure of the N-Terminal Domain of the Secretin GspD from ETEC Determined with the Assistance of a Nanobody

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674-693

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10.1038/NPROT.2014.039

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3

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9

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Serge Muyldermans

Søren G. F. Rasmussen

Alexandre Wohlkönig

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2014-02-27T00:00:00Z

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260430213

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1025604234

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24577359

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4297639

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